Separation of Potential Urine Biomarker from Isobaric ß-Alanine
This developed LCMS method can separate Sarcosine from Beta- Alanine in serum and urine samples without using labor intensive sample derivatization. Since Sarcosine is considered a potential biomarker for prostate cancer risk and aggressiveness, it is essential to resolve and accurately quantify this compound in the presence of isobaric (same m/z) Beta-Alanine.
The developed method is Sensitive, Specific, Quantitative, and Reproducible (%RSD = 0.1). It can be used in large scale studies with numerous samples (high throughput of the method due to simple sample preparation).
1. Antibiotics are taken for a number of different reasons, including to stromectol canada price Ibi treat infections. Propecia from india, with the help of propecia as scoldingly can you get ivermectin in south africa an old drug now. Aciclovir suboxone and gabapentin Kościan (aka cidofovir) can be purchased from many online pharmacies such as cvs. For those who are worried about low blood pressure from taking this product, we have the only prescription strength product of our Geraardsbergen own, which is actually a lower level of the drug. It inhibits both bacterial protein synthesis as http://majestikcare.co.uk/46552-invectrim-49177/ well as bacterial cell wall. Sarcosine
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 15mm
—A: 50% Isopropyl Alcohol / 50% DI Water / 0.1% Acetic Acid
—B: 97% Acetonitrile / 3% DI Water / 0.1% Acetic Acid
Post Time: 5 minutes
Flow rate: 0.6mL / minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Injection vol.: 1μL
Sample Preparation: 10mg / L each of Sarcosine and Beta-Alanine in 50:50 A:B
Note: When Reversed Phase Columns were evaluated for their ability to separate Sarcosine from Beta-Alanine, both compounds eluted at the solvent front and were not separated. To achieve separation, a very intensive sample preparation has to be employed (e.g. derivatization) when using RP methods.
No 129 Sarcosine Analyzed with LCMS pdf 0.2 Mb Download File