Will phosphates precipitate in an HPLC column – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 1, 2012
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Primer / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Reversed Phase Columns FAQ / Trouble Shooting Suggestions / TYPE-C in General FAQ

Phosphoric acid will not precipitate in the columns. Sodium phosphate (which is not recommended) but which is often used as a buffer, will precipitate in the columns and should be avoided as a storage solvent for all HPLC columns.

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1) Why use buffers during analysis and acid buffers such as the phosphoric acid for column storage solvents for Bidenate C18 columns? Will phosphate precipitate in the column INTERNAL ONLY

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