Why am I seeing two peaks for quaternium15 Dowicil-75 in LCMS – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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September 25, 2014
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Cogent Diamond Hydride Information / Diamond Hydride FAQ / Frequently Asked Questions?? / HPLC Column Information / HPLC Columns FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / Trouble Shooting Suggestions / TYPE-C Column Information

Question: I am using LC-MS and the Cogent Diamond Hydride™ HPLC column. When analyzing quaternium-15 sold as Dowicil-75™ , I see two peaks which have the same m/z. What can be the cause? Is there an issue of peak splitting?

Answer: Quaternium-15 can have either cis or trans orientation on the double bond. If you see two peaks in your data, it may not be from peak splitting but rather chromatographic separation of the two isomers.

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