Improvement in signal to noise in LCMS with an ANP method – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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September 25, 2013
Bill Ciccone

ANP Aqueous Normal Phase Articles / Cogent Diamond Hydride Information / Cogent Phenyl Hydride Information / Cogent UDA Information / Frequently Asked Questions?? / HPLC Column Information / HPLC Columns FAQ / HPLC Primer / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Reversed Phase Columns FAQ / TYPE-C Column Information / TYPE-C in General FAQ

“Signal-to-Noise” ratio can often be improved by using Aqueous Normal Phase ANP methods in LCMS instead of Reversed Phase RP methods. This results in improved limits of detection for a method making it more “sensitive”.

The simple reason is that Mass Spec is a gas state method and it is easier to fully and quickly evaporate organic solvents used in ANP than water which is the dominant solvent in Reversed Phase. Hence better signal to noise or in the vernacular, better sensitivity.

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