Convert HPLC columns between Reversed Phase and Normal Phase – How to

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 11, 2012
Bill Ciccone

How To... Information / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Reversed Phase Columns FAQ / Silica-C FAQ / TYPE-C Column Information / TYPE-C in General FAQ

To start work in different modes of HPLC, a simple switching procedure is recommended.
A simple 30 minute procedure allows switching from one mode to another.

Procedure:

A – Moving from Reversed Phase to Normal Phase HPLC; pump 100% methanol for 15 minutes at 1 mL / minute flow rate, followed by 15 minutes 100% methylene chloride. The column is ready to be equilibrated with mobile phase for NP-HPLC.

B – Moving from Normal Phase to Reversed Phase HPLC; pump 100% methylene chloride for 15 minutes at 1 mL / minute flow rate, followed by 15 minutes 100% methanol. The column is ready to be equilibrated with mobile phase for RP-HPLC.

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