Is the Cogent UDC Cholesterol HPLC column stable as a stationary phase – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 2, 2012
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Cogent UDC-Cholesterol Information / HPLC Column Information / TYPE-C Column Information / UDC-Cholesterol FAQ

While cholesterol is a large molecule, the UDC-Cholesterol phase has proven to be stable in 0.1% v/v TFA and 0.1% v/v H₃PO₄ at acid pH’s and up through pH 8.00 with 20mM NH₄COOH buffer with NH₄OH.

A pH in excess of pH 8.00 should be avoided as this phase has a mono dentate (single point) attachment to the silica matrix and an intermediate ester link in its structure.

For higher pH’s the Cogent Bidentate C18™ is recommended.

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