Dipyridamole Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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May 10, 2012
dcatri

AppNotes Cogent Bidentate C18 / AppNotes Library / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Several Tertiary Amines on a Molecule that is Easily Retained

This Method for the Analysis of Dipyridamole USP Tablet Formulation is easy to do. Note the excellent Resolution from capsule excipients, along with the excellent Peak shape for this complex, difficult molecule.

Peaks:
1. Dipyridamole
2. Tablet Excipients

Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
—A: DI Water / 0.1% Phosphoric Acid
—B: 80% Acetonitrile / 20% DI Water / 0.1% Phosphoric Acid
Gradient:

Time (minutes)	%B
0	0
15	100

Temperature: 40°C
Injection vol.: 10μL
Flow rate: 1.5mL / minute
Detection: UV @ 280nm

Notes: Dipyridamole is a drug that inhibits thrombus formation when given chronically and causes vasodilation when given at high doses over short time. Modified release Dipyridamole is used in conjunction with aspirin (under the trade names Aggrenox in the USA or Asasantin Retard in the UK) in the secondary prevention of stroke and transient Ischaemic attack. Dipyridamole absorption is pH-dependent and concomitant treatment with gastric acid suppressors (such as a proton pump inhibitor) will inhibit uptake significantly.

Notes: We recommend dedicating TYPE-C™ Columns to Methods once they have been used with Phosphates.

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