p-Toluenesulfonic Acid Monohydrate Analyzed with HPLC – AppNote

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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November 29, 2021
Tanya

AppNotes Cogent Phenyl Hydride / AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

A Reproducible Method for Analysis of an Oxonium Salt

A rapid, sensitive, and Reproducible Method has been developed for Analysis of p-Toluenesulfonic Acid Monohydrate. The data below, (an overlay of 5 chromatograms ) illustrates how the compound can be adequately Retained and detected using this straightforward Method.

A Phenyl ring in the Column Stationary Phase provides strategic use of π-π Interaction with the Analyte making possible the use of a very simple, Mass Spec-friendly Mobile Phase with Formic Acid as an additive.

5 Injections of p-Toluenesulfonic Acid Monohydrate

Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Dimensions: 4.6mm x 75mm
Mobile Phase: (85:15) DI Water / Acetonitrile with 0.1% Formic Acid
Injection vol.: 2μL
Flow rate: 1.0mL / minute
Detection: UV @ 210nm
Sample Preparation: p-Toluenesulfonic Acid Monohydrate prepared as 1.0mg / mL Standard Solution in DI Water

Notes: p-Toluenesulfonic Acid Monohydrate is widely used as catalyst agent in the synthesis of pharmaceuticals, pesticides, polymerization stabilizer and organic synthesis (esters, etc.), paint intermediates and resin curing agent.

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