Alternate L1 column used for a similar Hydrocortisone Assay with HPLC – AppNote

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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May 24, 2021
Tanya

AppNotes Alternate Generic Methods / AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / AppNotes RP C18 / ARTICLES, APPNOTES, FAQ

Alternate L1 column used for a similar USP assay method of Hydrocortisone

This Similar USP Hydrocortisone Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of 0.5%.

This demonstrates a good alternate Column for your Hydrocortisone USP Method.

Peak:

Hydrocortisone

Method Conditions:
Column: Cogent MicroBee C18™, 5um, 125Å
Catalog No.: 59305-03
Dimensions: 3.9mm x 300mm
Mobile Phase: 25:75 Acetonitrile / DI Water
Injection vol.: 15μL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Hydrocortisone 50μg / mL in Methanol

Note 1: Hydrocortisone, a Corticosteroid, and is similar to a natural hormone produced by your Adrenal Glands. Hydrocortisone is used to treat immune, inflammatory, and neoplastic conditions.

Note 2: The Cogent MicroBee C18™ performs very similarly to the Waters® µBondapak C18®.

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