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Hormone Replacement Assay Methods

Reference Number: AA-02684 Created: 07/24/2015 03:03 PM Last Updated: 09/12/2020 02:04 PM

In this extended application note, separation is demonstrated between various components in hormone replacement formulations. Often, these formulations have multiple active ingredients present. Therefore HPLC is an appropriate technique in obtaining the necessary specificity for a quantitative assay. The Cogent TYPE-C Silica™ columns can be effective for achieving these analytical goals in a robust and reliable manner.
The Cogent Bidentate C8 and C18™ columns retain the hormones by conventional reversed phase interactions, while the UDC-Cholesterol™ can also differentiate among the compounds by shape. The application delineates these chromatographic strategies for two brands of hormone replacement pharmaceuticals, Femhrt® and Bi-Est®.


See downloadable pdf for full study.


1. Ethinylestradiol
2. Norethisterone acetate


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