Adjusted retention time hold up time – Primer – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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May 15, 2013
Bill Ciccone

ARTICLES, APPNOTES, FAQ / HPLC Primer / HPLC Suggestions and Information / HPLC Tips and Suggestions / Trouble Shooting Suggestions

In the chart below, t_R is the retention time and t_M is the hold-up time.

Definition: the hold-up time is the time of an analyte (small molecule) which completely penetrates the pores and which is not retained at all by the stationary phase.

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