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MicroSolv manufactures HPLC columns, autosampler vials for HPLC & GC, syringe filters, fittings and accessories and small bore tubing. CE, TLC, Flash Chromatography and OPLC are also available. Separation sciences leaders.

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How to condition Cogent Type-B columns for storage

Reference Number: AA-00418 Created: 04/13/2012 09:30 PM Last Updated: 04/28/2012 04:21 PM

Long Term Storage:

  1. Rinse the column with 8 column volumes with pure solvent that you use as your mobile phase. It is important to use non-buffered mobile phase.
  2. Then purge the column with 8 column volumes of 100% HPLC Grade, filtered Isopropyl Alcohol (IPA).
  3. Then purge the column with 80% IPA and 20% water with 8 column volumes.
  4. Cap both ends of the column with the provided protective end caps or with Super-Link (Seal-Links) End Fittings and capping them with column plugs provided.
  5. Return column to the box it came in for safekeeping.
  6. Store column at room temperature.
  7. Never let the column dry out.
  8. Never store the column in buffers.
  9. Never store the column in liquid that is more than 30% water.

 

Overnight:

    1. Rinse the column with 8 column volumes with pure solvent that you use as your mobile phase. It is important to use non-buffered mobile phase.
    2. Cap both ends of the column with the provided protective end caps or with Super-Link End Fittings and capping them with column plugs provided.

       

       

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