HPLC Column, Phenyl Hydride 2.o, 2.2um, 2.1mm ID x 50mm Length, 120A, High Pressure. Cogent TYPE-C Brand. 1 EA.
Unique Phenyl Selectivity for Polar and Aromatic Compounds
TYPE‑C™ Silica | RP & ANP Modes | Excellent for Peptides and LC‑MS
Phenyl Hydride™ HPLC columns are one of MICROSOLV’s most unique and best‑performing stationary phases, delivering selectivity and durability that cannot be achieved with conventional phenyl columns. Built on TYPE‑C™ silica hydride technology, these columns provide exceptional performance for LC‑MS, aqueous normal phase (ANP / HILIC‑style), and reversed‑phase (RP) separations.
Phenyl Hydride™ columns are widely used for peptides, polar compounds, and aromatic analytes, making them a top choice for laboratories that require high stability, fast equilibration, and reproducible gradients.
Proprietary TYPE‑C™ Phenyl Hydride Chemistry
Phenyl Hydride™ columns are manufactured using MICROSOLV’s proprietary bonding technology, which creates a direct silicon–carbon (Si–C) bond between the stationary phase ligand and the silica surface. The phenyl group is attached via a straight‑chain, four‑carbon (C4) linker, resulting in an exceptionally stable structure.
This chemistry provides:
Superior chemical and mechanical durability
High efficiency and long column lifetime
Minimal surface reactivity
Stable performance across multiple chromatographic modes
Unlike conventional phenyl columns, Phenyl Hydride™ columns are non‑end capped, yet remain highly stable due to the underlying silica hydride surface.
Multi‑Mode Operation: RP, NP, and ANP / HILIC
One of the most important advantages of Cogent Phenyl Hydride™ is its ability to operate effectively in multiple separation modes:
Reversed‑Phase (RP) HPLC
Aqueous Normal Phase (ANP / HILIC style)
Organic Normal Phase (ONP)
This flexibility allows a single column chemistry to be used across different methods and analyte classes, reducing the need for multiple column types in the laboratory.
Unique Selectivity for Aromatic and Polar Compounds
Phenyl Hydride™ columns provide distinct selectivity for compounds containing aromatic rings, driven by π–π interactions combined with the unique surface chemistry of TYPE‑C™ silica.
They are especially effective for separating:
Small peptides
Aromatic pharmaceuticals
Sulfonyl compounds
Azides
Other polar and aromatic molecules
This makes Phenyl Hydride™ a powerful alternative when traditional phenyl, C18, or HILIC columns do not deliver adequate resolution.
Ideal for LC‑MS Applications
Chromatographers strongly favor Phenyl Hydride™ columns for LC‑MS due to their non‑end capped design. Because there are no end‑capping reagents to bleed, these columns offer:
Cleaner MS baselines
Reduced background noise
Improved signal stability
Better long‑term MS performance
This advantage, combined with fast equilibration and run‑to‑run precision, makes Phenyl Hydride™ an excellent choice for gradient methods and high‑throughput LC‑MS workflows.
Key Features and Benefits
TYPE‑C™ silica hydride phenyl phase
Direct Si–C bonded ligand for exceptional durability
Non‑end capped, MS‑friendly design
Operates in RP, ANP (HILIC‑style), and ONP
Unique selectivity for aromatic and polar compounds
Excellent for peptides and small polar molecules
Fast equilibration and high run‑to‑run reproducibility
Proven performance in HPLC and LC‑MS
Typical Applications
LC‑MS and LC‑MS/MS
Peptide analysis
Polar and aromatic compounds
ANP / HILIC style separations
Pharmaceutical and bioanalytical research
Gradient method development
Additional Resources
Column Protection Recommendation
Important: MICROSOLV technical support strongly recommends using the Cogent Column Filter, which is included with each column, whenever possible. Column filters help protect against particulates, extend column lifetime, and maintain consistent chromatographic performance.
👉 Learn more about the Cogent Column Filter for HPLC column protection
