HPLC Column, Phenyl Hydride, 4um, 2.1mm ID x 150mm Length, 100A. Cogent TYPE-C Brand. 1 EA.

Additional Info:

High‑Resolution HILIC Separations with Aromatic Selectivity Using Phenyl Hydride™

The 69020‑15P‑2 Phenyl Hydride™ HPLC Column is designed for laboratories requiring enhanced aromatic selectivity, high resolving power, and efficient narrow‑bore operation. Manufactured with Cogent TYPE‑C™ silica hydride technology, the Phenyl Hydride stationary phase delivers excellent selectivity, fast equilibration, and long column lifetime for compounds that are difficult to retain or separate using conventional reversed‑phase methods.

This column format is frequently selected for LC‑MS applications where increased resolution and analytical confidence are required.

Why Choose Phenyl Hydride™ for HILIC Applications

Phenyl Hydride™ stationary phases combine classic HILIC retention with interaction mechanisms well‑suited for aromatic compounds:

  • Strong retention of polar analytes under HILIC conditions

  • π–π and hydrophobic interactions that improve separation of aromatic species

  • Enhanced resolution for analytes poorly retained in reversed‑phase systems

  • Fast equilibration and highly reproducible retention behavior

These characteristics make Phenyl Hydride an effective choice for both routine analysis and demanding method‑development work.

Cogent TYPE‑C™ Silica Hydride Advantage

Phenyl Hydride™ columns are based on silica hydride surfaces rather than traditional silanol‑rich silica, providing important performance advantages:

  • Faster equilibration than bonded‑silica HILIC phases

  • Stable retention at high organic mobile‑phase composition

  • Reduced phase collapse and improved robustness

  • Extended column lifetime in routine analytical workflows

These benefits support consistent performance across extended analytical sequences.

Ideal Applications

The 69020‑15P‑2 Phenyl Hydride column is routinely used for:

  • Aromatic amines

  • Small peptides containing aromatic residues

  • Azides and heteroaromatic compounds

  • Polar analytes requiring high‑resolution HILIC separations

It is particularly valuable when closely eluting aromatic compounds demand increased resolving power.

Narrow‑Bore 150 mm Column for Maximum Resolution

The 2.1 mm ID × 150 mm length configuration provides:

  • High plate count for separation of closely eluting compounds

  • Increased resolution compared to shorter narrow‑bore columns

  • Reduced solvent consumption relative to 4.6 mm ID analytical columns

This format is commonly chosen for LC‑MS methods where resolution and sensitivity are both critical.

LC‑MS and UV Detection Considerations

This column is well‑suited for LC‑MS workflows. Because of the 2.1 mm internal diameter, optimal performance requires:

  • An HPLC or UHPLC system capable of accurate, stable low‑flow delivery

  • Minimized system dead volume

  • Low‑volume flow cells when using UV detection

Systems without reliable low‑flow capability may not be suitable.

Operating Guidelines and System Requirements

  • Typical flow‑rate range: ~0.2–0.3 mL/min (method‑dependent)

  • System compatibility: HPLC or UHPLC systems capable of stable low‑flow operation

  • Special requirements: Low‑volume detector flow cell recommended

Flow rates may be adjusted based on resolution goals, solvent composition, and pressure limitations.

 

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