Cogent HPS™ Silica High‑Purity Columns for Fats & Lipids

High‑Purity Silica Columns for Normal‑Phase and USP® L‑03 Methods

Unmodified Silica | Excellent for Fats and Fat‑Soluble Compounds

Cogent HPS™ Silica HPLC columns are engineered for normal‑phase (NP) HPLC and USP® L‑03 methods, delivering reliable performance for the separation of fats, fat‑soluble compounds, and other polar analytes. These columns are manufactured using proprietary, high‑purity silica with extremely low metal content, resulting in durable, efficient, and reproducible separations.

Cogent HPS™ Silica columns are a trusted choice for laboratories running routine, regulated, and legacy silica‑based methods.

High‑Purity, Unmodified Silica for Reliable Separations

Cogent HPS™ Silica columns are made with unmodified, high‑purity silica (HPS™) featuring a narrow particle size distribution. The silica is carefully controlled to maintain metal content below 1 ppm, which helps reduce undesirable interactions and improves column durability.

This controlled silica surface provides:

• Consistent retention behavior

• Improved efficiency

• Long column lifetime

• Reproducible performance from lot to lot

These characteristics make Cogent HPS™ Silica well suited for both analytical and routine applications.

Designed for Normal‑Phase HPLC — with HILIC Flexibility

Silica columns are most commonly used in normal‑phase HPLC, typically with non‑polar mobile phases. Cogent HPS™ Silica columns excel in this mode and are particularly effective for compounds that are poorly retained on reversed‑phase columns.

In addition to NP use, Cogent HPS™ Silica columns can also be operated in HILIC mode using HPLC‑grade acetonitrile and water, providing added flexibility for laboratories working with highly polar analytes.

Ideal for Fat‑Soluble and Polar Compounds

Cogent HPS™ Silica columns are commonly selected for the separation of:

• Fats and lipids

• Fat‑soluble vitamins

• Steroidal compounds

• Polar organic molecules

• Compounds requiring USP® L‑03 silica phases

The unmodified silica surface provides strong polar interactions, making these columns especially effective when alkyl phases do not provide adequate retention.

Key Features and Benefits

• Designed for USP® L‑03 silica methods

• Optimized for normal‑phase HPLC

• Unmodified high‑purity silica (HPS™)

• 5 µm particle size with narrow distribution

• Metal content below 1 ppm

• Efficient, durable, and reproducible performance

• Can be used in NP or HILIC modes

• Reliable option for routine and legacy methods

Typical Applications

• Normal‑phase HPLC separations

• USP® L‑03 compliant methods

• Fats and fat‑soluble vitamins

• Steroids and lipid‑like compounds

• Polar compound separations

• Analytical and routine laboratory workflows

Additional Resources

👉 Learn more about the Cogent HPS™ HPLC column family

Column Protection Recommendation

Important: MICROSOLV technical support strongly recommends using the Cogent Column Filter, which is included with each column, whenever possible. Column filters help protect against particulates, extend column lifetime, and maintain consistent chromatographic performance.

👉 Learn more about the Cogent Column Filter for HPLC column protection