Cogent HPS™ Phenyl for Aromatic and Peptide Separations

High‑Purity Phenyl Columns with Excellent Peak Shape

Double End‑Capped | Unique Selectivity | Reversed‑Phase HPLC

Cogent HPS™ Phenyl HPLC columns are engineered for high efficiency, excellent peak shape, and reliable selectivity in reversed‑phase (RP) HPLC. These columns are manufactured using proprietary, high‑purity silica and are designed to deliver consistent performance for both in‑house and USP methods.

With decades of proven laboratory use, Cogent HPS™ Phenyl remains a trusted choice for analysts who require clean separations of aromatic compounds and peptides.

Advanced Phenyl Chemistry on High‑Purity Silica

Cogent HPS™ Phenyl columns are base‑deactivated and mono‑functional bonded, helping minimize secondary interactions that can lead to peak tailing. The stationary phase is exhaustively double end‑capped, further reducing residual silanol activity and improving peak symmetry.

This carefully controlled surface chemistry results in:

• Improved peak shape

• Enhanced resolution

• Consistent retention behavior

• Reliable performance across routine RP methods

Designed for Reversed‑Phase HPLC

Cogent HPS™ Phenyl columns are packed and ready for reversed‑phase HPLC, offering aromatic selectivity through π–π interactions while maintaining robust hydrophobic retention. These characteristics make them especially useful for analytes that do not separate well on C18 columns.

For more than 20 years, laboratories have successfully used Cogent HPS™ Phenyl columns for routine QC, research, and USP‑based methods, making them a proven and dependable phenyl phase.

Ideal for Aromatic Compounds and Peptides

Cogent HPS™ Phenyl columns are particularly well suited for:

• Aromatic and polyaromatic compounds

• Pharmaceutical intermediates and impurities

• Peptide analysis

• Structurally rigid or conjugated molecules

The balanced selectivity of the phenyl phase provides reliable alternatives when alkyl phases lack sufficient resolution.

Available Formats and Configurations

Cogent HPS™ Phenyl columns are available to support both analytical and scale‑up workflows:

• Analytical columns

• Guard columns

• Preparative columns

• 5 µm particle size

• 120 Å pore size

This flexibility allows method transfer from analytical to preparative HPLC without changing selectivity.

Key Features and Benefits

• Manufactured with high‑purity silica

• Base‑deactivated surface chemistry

• Mono‑functional bonding

• Exhaustive double end‑capping

• Excellent peak shape and resolution

• Optimized for reversed‑phase HPLC

• Proven performance in USP and legacy methods

• Suitable for aromatic compounds and peptides

• Available in analytical, guard, and prep formats

Typical Applications

• Aromatic pharmaceuticals

• Polyaromatic compounds

• Peptide separations

• USP® and legacy reversed‑phase methods

• Research, QC, and GMP laboratories

Additional Resources

• View Cogent HPS™ Phenyl column specifications

• Learn more about the Cogent HPS™ HPLC column family

Column Protection Recommendation

Important: MICROSOLV technical support strongly recommends using the Cogent Column Filter, which is included with each column, whenever possible. Column filters help protect against particulates, extend column lifetime, and maintain consistent chromatographic performance.

👉 Learn more about the Cogent Column Filter for HPLC column protection