Catalog Number - 75020-SP50

Cogent HPS Phenyl™ Semi-Preparative HPLC Columns are designed for purification, fraction collection, and method scale-up of aromatic and structurally related compounds using reversed-phase chromatography. Manufactured using a monofunctional four-carbon ligand with an attached phenyl group bonded to high-purity 120Å silica and fully end capped for excellent peak shape and reproducibility, this stationary phase provides unique aromatic selectivity through π-π interactions. HPS Phenyl™ is particularly effective for compounds that may be difficult to separate on conventional C18 phases and is widely used for purification and isolation of complex aromatic analytes.

75020-SP50 is packed with 5 µm HPS Phenyl™ particles in a 10 mm ID x 50 mm semi-preparative format. The 5 µm particle size provides excellent chromatographic efficiency while maintaining lower operating backpressure than smaller particles. The 10 mm internal diameter increases sample loading capacity compared to analytical columns, while the short 50 mm column length is optimized for rapid purification, high-throughput processing, and semi-preparative method development.

The HPS Phenyl™ stationary phase is especially useful for purification of positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. The unique aromatic selectivity often provides improved separation of structurally similar compounds when compared to traditional alkyl-bonded stationary phases. The short column length makes this format ideal for rapid purification workflows, scouting studies, and analytical-to-semi-preparative method transfer.

Key Benefits

• HPS Phenyl™ stationary phase

• Fully end capped

• Monofunctional phenyl bonded phase

• High-purity 120Å silica

• 5 µm particle size

• Enhanced aromatic selectivity

• Excellent for positional isomer purification

• Lower operating backpressure

• Increased loading capacity for purification

• Ideal for rapid semi-preparative separations

Typical Applications

• Semi-preparative HPLC

• Compound purification

• Positional isomer purification

• Tocopherol purification

• Flavonoid purification

• Polynuclear aromatic compound isolation

• Nitro-aromatic compound purification

• API purification

• Natural product purification

• Reversed-phase chromatography

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: HPS Phenyl™

• Chromatography Mode: Reversed Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 10 mm ID x 50 mm

• Recommended Flow Rate: 4–8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• Fully end capped stationary phase

• Provides unique aromatic and π-π interaction selectivity

• Excellent for positional isomer purification

• Ideal for flavonoids, tocopherols, and aromatic compounds

• Increased loading capacity compared to analytical columns

• 10 mm ID format requires a semi-preparative capable HPLC system

• 50 mm length enables rapid purification and method development

• Lower backpressure compared to smaller particle columns

• Useful when additional selectivity beyond C18 phases is required

• This is a Special Order Item