Catalog Number - 75020-P250

Cogent HPS Phenyl™ Preparative HPLC Columns are designed for high-capacity purification of aromatic and structurally related compounds using reversed-phase chromatography. Manufactured using a monofunctional four-carbon ligand with an attached phenyl group bonded to high-purity 120Å silica and fully end capped for excellent peak shape and reproducibility, this stationary phase provides unique aromatic selectivity through π-π interactions. HPS Phenyl™ is particularly effective for compounds that may be difficult to separate on conventional C18 phases and is widely used for purification and scale-up applications involving complex aromatic analytes.

75020-P250 is packed with 5 µm HPS Phenyl™ particles in a 21.2 mm ID x 250 mm preparative format. The 5 µm particle size provides excellent chromatographic efficiency with lower operating backpressure than smaller particles, while the large 21.2 mm internal diameter delivers substantial sample loading capacity. The 250 mm column length provides maximum retention, resolution, and peak capacity for demanding preparative separations and compound isolation workflows.

The HPS Phenyl™ stationary phase is especially useful for purification of positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. Its unique aromatic selectivity frequently provides improved separation of structurally related compounds compared to traditional alkyl-bonded stationary phases, making it an excellent choice for difficult preparative purifications and process development projects.

Key Benefits

• HPS Phenyl™ stationary phase

• Fully end capped

• Monofunctional phenyl bonded phase

• High-purity 120Å silica

• 5 µm preparative particle size

• Enhanced aromatic selectivity

• Excellent for positional isomer purification

• Lower operating backpressure

• High loading capacity for purification

• Maximum resolution and peak capacity

Typical Applications

• Preparative HPLC

• Compound purification

• Positional isomer purification

• Tocopherol purification

• Flavonoid purification

• Polynuclear aromatic compound isolation

• Nitro-aromatic compound purification

• API purification

• Natural product purification

• Reversed-phase chromatography

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: HPS Phenyl™

• Chromatography Mode: Reversed Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 21.2 mm ID x 250 mm

• Recommended Flow Rate: 15–30 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• Fully end capped stationary phase

• Provides unique aromatic and π-π interaction selectivity

• Excellent for positional isomer purification

• Ideal for flavonoids, tocopherols, and aromatic compounds

• High loading capacity for preparative applications

• 21.2 mm ID format requires a specialized preparative HPLC system

• 250 mm length provides maximum retention, resolution, and peak capacity

• Lower backpressure compared to smaller particle columns

• Useful when additional selectivity beyond C18 phases is required

• This is a Special Order Item