HPLC Column, Phenyl Hydride, 4um, 4.6mm ID x 50mm Length, 100A, Cogent TYPE-C Brand. 1 EA.
Additional Info:
Fast HILIC Performance with Aromatic Selectivity Using Phenyl Hydride™
The 69020‑05P Phenyl Hydride™ HPLC Column is designed for laboratories requiring enhanced aromatic selectivity combined with rapid HILIC equilibration. Manufactured with Cogent TYPE‑C™ silica hydride technology, the Phenyl Hydride stationary phase delivers stable retention, excellent peak shape, and long column lifetime for compounds that are difficult to retain or resolve using conventional reversed‑phase approaches.
While Diamond Hydride™ and Phenyl Hydride™ columns both excel in HILIC mode, the Phenyl Hydride phase introduces phenyl‑based interactions that make it particularly effective for analytes containing aromatic or heteroaromatic functionality.
Why Choose Phenyl Hydride™ for HILIC Separations
The Phenyl Hydride stationary phase offers complementary selectivity to Diamond Hydride™:
Retains highly polar analytes under HILIC conditions
Adds π–π and hydrophobic interactions for aromatic compounds
Improves resolution for analytes poorly retained in reversed phase
Maintains fast equilibration and reproducible retention
This dual‑interaction behavior makes Phenyl Hydride an excellent alternative or orthogonal option during method development.
Cogent TYPE‑C™ Silica Hydride Advantage
Like all Cogent TYPE‑C™ phases, Phenyl Hydride is built on silica hydride rather than conventional silanol‑rich silica, providing key performance benefits:
Faster equilibration compared to bonded‑silica HILIC phases
Stable retention mechanisms at high organic content
Reduced phase collapse and enhanced robustness
Extended column lifetime for routine analytical use
These characteristics support reproducible HILIC separations without excessive re‑equilibration time.
Ideal Applications
The 69020‑05P Phenyl Hydride column is frequently selected for:
Aromatic amines
Small peptides containing aromatic residues
Azides and heteroaromatic compounds
Polar molecules poorly retained by reversed‑phase columns
It is particularly effective when traditional C18 or polar‑embedded phases fail to deliver adequate retention or selectivity.
Short‑Length Column for Fast Analysis
The 4.6 mm ID × 50 mm length format is optimized for:
Short run times and increased sample throughput
Screening and method development workflows
Applications where fast results are prioritized over maximum peak capacity
Despite its reduced length, the column provides good resolution and robust performance, making it a popular choice for high‑throughput screening environments.
HPLC, UV, and LC‑MS Compatibility
This column is fully compatible with:
Conventional analytical HPLC systems
UV detection using standard flow cells
LC‑MS workflows where standard analytical flow rates are acceptable
The standard 4.6 mm internal diameter supports traditional injection volumes and detector configurations without specialized low‑dead‑volume hardware.
Operating Guidelines and System Requirements
Typical flow‑rate range: ~0.8–1.5 mL/min (method‑dependent)
System compatibility: All conventional analytical HPLC systems
Special requirements: None; no low‑flow capability required
Flow rates may be optimized based on resolution requirements, solvent composition, and pressure limits.