Catalog Number - 69020-25P

Maximum‑Resolution HILIC Separations with Aromatic Selectivity Using Phenyl Hydride™

The 69020‑25P Phenyl Hydride™ HPLC Column is designed for laboratories that require the highest resolving power and extended retention for polar and aromatic compounds. Manufactured with Cogent TYPE‑C™ silica hydride technology, the Phenyl Hydride stationary phase delivers excellent selectivity, fast equilibration relative to column length, and long column lifetime for analytes that are difficult to retain or separate using conventional reversed‑phase methods.

This column format is selected when closely related compounds require additional separation or when longer retention times improve analytical confidence.

Why Choose Phenyl Hydride™ for High‑Resolution HILIC Applications

Phenyl Hydride™ stationary phases combine classic HILIC retention with interaction mechanisms favorable for aromatic compounds:

• Strong retention of highly polar analytes under HILIC conditions

• π–π and hydrophobic interactions that enhance aromatic discrimination

• Improved separation of structurally similar or closely eluting compounds

• Fast equilibration and highly reproducible retention behavior

These characteristics make Phenyl Hydride particularly effective for challenging separations where resolution is the primary goal.

Cogent TYPE‑C™ Silica Hydride Advantage

Phenyl Hydride™ columns are based on silica hydride surfaces rather than traditional silanol‑rich silica, providing important performance advantages even at extended column lengths:

• Faster equilibration than bonded‑silica HILIC phases

• Stable retention at high organic mobile‑phase composition

• Reduced phase collapse and improved robustness

• Extended column lifetime for routine and high‑demand analytical workflows

These features support consistent, long‑run performance with minimal variability.

Ideal Applications

The 69020‑25P Phenyl Hydride column is routinely used for:

• Aromatic amines

• Small peptides containing aromatic residues

• Azides and heteroaromatic compounds

• Polar analytes that require extended retention or enhanced resolution

It is especially valuable in methods where peak spacing and compound discrimination are critical.

250 mm Column Length for Maximum Separation

The 4.6 mm ID × 250 mm length configuration provides:

• Maximum plate count for separation of closely related compounds

• Increased retention for very polar analytes

• Robust performance in complex mixtures requiring extended separation

This format is commonly chosen for difficult separations where shorter columns do not provide sufficient resolving power.

HPLC and UV Detection Compatibility

This column is fully compatible with:

• Conventional analytical HPLC systems

• UV detection using standard flow cells

The 4.6 mm internal diameter supports traditional injection volumes and detector configurations without specialized hardware.

Operating Guidelines and System Requirements

• Typical flow‑rate range: ~0.8–1.5 mL/min (method‑dependent)

• System compatibility: All conventional analytical HPLC systems

• Special requirements: None

Flow rates may be adjusted to balance resolution, analysis time, and system pressure limits.