Catalog Number - 69020-05P-2

Fast HILIC and Aromatic Selectivity in a Narrow‑Bore Phenyl Hydride™ Format

The 69020‑05P‑2 Phenyl Hydride™ HPLC Column is engineered for laboratories that need strong aromatic selectivity, rapid equilibration, and high sensitivity in a compact analytical format. Manufactured with Cogent TYPE‑C™ silica hydride technology, the Phenyl Hydride stationary phase provides stable retention, excellent peak shape, and long column lifetime for analytes that are difficult to retain or resolve using traditional reversed‑phase chemistries.

While Phenyl Hydride™ and Diamond Hydride™ columns are both highly effective in HILIC mode, the Phenyl Hydride phase introduces phenyl‑based interactions that enhance selectivity for aromatic and heteroaromatic compounds.

Why Choose Phenyl Hydride™ in HILIC Mode

The Phenyl Hydride stationary phase combines classic HILIC retention with additional interaction mechanisms:

• Strong retention of polar analytes under HILIC conditions

• π–π and hydrophobic interactions for improved aromatic discrimination

• Enhanced resolution for analytes poorly retained in reversed phase

• Fast equilibration and reproducible retention behavior

This makes Phenyl Hydride an excellent complementary option during method development when Diamond Hydride™ or reversed‑phase columns lack optimal selectivity.

Cogent TYPE‑C™ Silica Hydride Advantage

Phenyl Hydride™ columns are built on silica hydride surfaces rather than traditional silanol‑rich silica, delivering important performance benefits:

• Faster equilibration compared to bonded‑silica HILIC phases

• Stable retention mechanisms at high organic content

• Reduced phase collapse and improved robustness

• Extended column lifetime for routine analytical workflows

These characteristics support consistent performance across high‑throughput and LC‑MS applications.

Ideal Applications

The 69020‑05P‑2 Phenyl Hydride column is commonly selected for:

• Aromatic amines

• Small peptides containing aromatic residues

• Azides and heteroaromatic compounds

• Polar analytes that are difficult to retain using reversed‑phase columns

It is particularly useful when aromatic selectivity is critical to achieving acceptable resolution.

Short, Narrow‑Bore Column for Fast, Sensitive Analysis

The 2.1 mm ID × 50 mm length configuration provides clear performance advantages:

• Short run times with reduced solvent consumption

• Increased sensitivity due to narrow‑bore geometry

• Ideal for screening, rapid method development, and LC‑MS workflows

Because of the reduced internal diameter, this format requires instrumentation capable of precise low‑flow delivery and detector flow cells designed for small volumes to maintain peak integrity.

LC‑MS Compatibility and System Considerations

This column size is a popular and well‑established choice for LC‑MS when operated under HILIC conditions. For optimal performance:

• The HPLC or UHPLC system must deliver reliable low flow rates

• System dead volume should be minimized

• Detector and MS interfaces should be optimized for narrow‑bore columns

Standard analytical HPLC systems without accurate low‑flow capability may not be suitable.

Operating Guidelines and System Requirements

• Typical flow‑rate range: ~0.2–0.3 mL/min (method‑dependent)

• System compatibility: HPLC or UHPLC systems capable of stable low‑flow operation

• Special requirements: Low‑volume flow cell recommended for UV detection

Flow rates may be adjusted based on resolution needs, solvent composition, and pressure limits.