Semi Prep HPLC Column, Phenyl Hydride, 4um, 100A. 10mm ID x 100mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
Semi‑Preparative HILIC Purification with Aromatic Selectivity Using Phenyl Hydride™
The 69020‑SP100 Phenyl Hydride™ Semi‑Preparative HPLC Column is designed for laboratories performing semi‑preparative purification of aromatic and polar compounds that are difficult to retain or resolve using conventional reversed‑phase chromatography. Manufactured using Cogent TYPE‑C™ silica hydride technology, this stationary phase delivers excellent selectivity, fast equilibration, and robust column lifetime while supporting higher sample loads than analytical formats.
This column is selected when additional loading capacity and improved resolution are required beyond analytical columns, without transitioning to full preparative diameters.
Why Choose Phenyl Hydride™ for Semi‑Preparative Aromatic Separations
Phenyl Hydride™ stationary phases combine HILIC‑type retention with interaction mechanisms that favor aromatic compounds, making them particularly effective for semi‑preparative workflows:
Strong retention of polar and moderately polar analytes under high‑organic mobile‑phase conditions
π–π and hydrophobic interactions that enhance aromatic selectivity
Improved separation of structurally similar compounds
Reproducible retention behavior across repeated injections
These characteristics make Phenyl Hydride™ well suited for isolations where purity and selectivity are critical prior to scale‑up or downstream use.
Cogent TYPE‑C™ Silica Hydride Advantage
Phenyl Hydride™ columns are based on silica hydride surfaces rather than traditional silanol‑rich bonded silica, providing important performance benefits for semi‑preparative applications:
Faster equilibration compared to conventional bonded‑silica HILIC phases
Stable retention at high organic solvent composition
Reduced susceptibility to phase collapse
Extended column lifetime under repeated semi‑preparative use
These features ensure consistent performance and predictable retention across method development and purification workflows.
Ideal Applications
The 69020‑SP100 Phenyl Hydride™ semi‑preparative column is commonly used for purification of:
Aromatic amines
Small peptides containing aromatic residues
Azides and heteroaromatic compounds
Polar analytes poorly retained by reversed‑phase methods
It is particularly valuable when increased load capacity and improved selectivity are required beyond analytical HPLC.
10 mm ID × 100 mm Length for Semi‑Preparative Purification
The 10 mm internal diameter × 100 mm length configuration provides:
Increased sample‑loading capacity compared to analytical columns
Efficient separations with moderate backpressure
Improved resolution and recovery for semi‑preparative isolations
This format is commonly chosen for milligram‑scale purification and method scale‑up.
System Compatibility and Operating Requirements
Due to its diameter and operating demands, this column requires a specialized HPLC system capable of supporting semi‑preparative operation, including:
Reliable delivery of elevated flow rates
Appropriate injector and detector configurations
Pressure and solvent handling suitable for larger‑ID columns
Typical flow‑rate range: Method‑dependent; based on system capability System compatibility: Semi‑preparative or preparative‑capable HPLC systems Special requirements: Standard analytical‑only HPLC systems may not be suitable
Custom Order Manufacturing Notice
The 69020‑SP100 Phenyl Hydride™ Semi‑Preparative Column:
Can be manufactured to order
Is supplied according to Custom Order specifications
Is intended for users who have verified system compatibility and application fit
Customers are encouraged to review Custom Order information prior to purchase.