HPLC Column, Phenyl Hydride, 4um, 4.6mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
Balanced HILIC Performance with Aromatic Selectivity Using Phenyl Hydride™
The 69020‑10P Phenyl Hydride™ HPLC Column is designed for laboratories that require enhanced aromatic selectivity with moderate run times and improved resolution. Manufactured with Cogent TYPE‑C™ silica hydride technology, the Phenyl Hydride stationary phase delivers excellent selectivity, fast equilibration, and long column lifetime for analytes that are difficult to retain or resolve using conventional reversed‑phase methods.
This column format is frequently selected when shorter 50 mm columns do not provide sufficient resolution, but faster analysis is still desired compared to longer analytical columns.
Why Choose Phenyl Hydride™ for HILIC Applications
Phenyl Hydride™ columns combine classic HILIC retention with interaction mechanisms favorable for aromatic compounds:
Strong retention of polar analytes under HILIC conditions
π–π and hydrophobic interactions that enhance separation of aromatic species
Improved resolution for analytes poorly retained in reversed‑phase separations
Fast equilibration and highly reproducible retention behavior
These characteristics make Phenyl Hydride an effective choice for both routine analysis and method development involving aromatic functionality.
Cogent TYPE‑C™ Silica Hydride Advantage
Phenyl Hydride™ columns are based on silica hydride surfaces rather than traditional silanol‑rich silica, providing measurable performance benefits:
Faster equilibration than bonded‑silica HILIC phases
Stable retention at high organic mobile‑phase content
Reduced phase collapse and improved robustness
Extended column lifetime in routine analytical use
These features support consistent performance across repeated injections and analytical batches.
Ideal Applications
The 69020‑10P Phenyl Hydride column is commonly used for:
Aromatic amines
Small peptides containing aromatic residues
Azides and heteroaromatic compounds
Polar analytes that are difficult to retain or separate using reversed‑phase columns
It is especially useful where added aromatic selectivity improves peak resolution and confidence.
100 mm Column Length for Speed‑to‑Resolution Balance
The 4.6 mm ID × 100 mm length configuration provides a practical balance of speed and resolving power:
Shorter run times than 150 mm analytical columns
Higher resolution than 50 mm columns for closely eluting compounds
Robust performance for routine analytical workflows
This format is often chosen for validated methods and applications requiring both efficiency and resolution.
HPLC, UV, and LC‑MS Compatibility
This column is compatible with:
Conventional analytical HPLC systems
UV detection using standard flow cells
LC‑MS workflows where standard analytical flow rates are acceptable
The 4.6 mm internal diameter supports traditional injection volumes and detector configurations without specialized hardware requirements.
Operating Guidelines and System Requirements
Typical flow‑rate range: ~0.8–1.5 mL/min (method‑dependent)
System compatibility: All conventional analytical HPLC systems
Special requirements: None; no low‑flow capability required
Flow rates may be optimized based on resolution targets, solvent composition, and system pressure limits.