HPLC Column, Phenyl Hydride, 4um, 2.1mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
High‑Resolution HILIC with Aromatic Selectivity Using Phenyl Hydride™
The 69020‑10P‑2 Phenyl Hydride™ HPLC Column is designed for laboratories requiring enhanced aromatic selectivity, increased resolution, and efficient narrow‑bore operation. Manufactured with Cogent TYPE‑C™ silica hydride technology, the Phenyl Hydride stationary phase delivers excellent selectivity, faster equilibration, and robust column lifetime for analytes that are difficult to retain or resolve using conventional reversed‑phase methods.
This column size is frequently selected when higher resolution is required than short columns can provide, while still maintaining efficient run times and low solvent consumption.
Why Choose Phenyl Hydride™ for HILIC Applications
Phenyl Hydride™ stationary phases combine classic HILIC retention with interaction mechanisms favorable for aromatic compounds:
Strong retention of polar analytes under HILIC conditions
π–π and hydrophobic interactions that enhance aromatic discrimination
Improved resolution for analytes poorly retained in reversed‑phase systems
Fast equilibration and reproducible retention behavior
These properties make Phenyl Hydride particularly effective for method development and routine analysis involving aromatic or heteroaromatic functionality.
Cogent TYPE‑C™ Silica Hydride Advantage
Phenyl Hydride™ columns are based on silica hydride surfaces rather than traditional silanol‑rich silica, providing clear performance advantages:
Faster equilibration than bonded‑silica HILIC phases
Stable retention at high organic mobile‑phase composition
Reduced phase collapse and enhanced robustness
Extended column lifetime during routine analytical use
These features support consistent and reliable separations with minimal downtime.
Ideal Applications
The 69020‑10P‑2 Phenyl Hydride column is routinely used for:
Aromatic amines
Small peptides containing aromatic residues
Azides and heteroaromatic compounds
Polar analytes that are difficult to retain or separate using reversed‑phase methods
It is especially valuable where added aromatic selectivity and improved resolution enhance analytical confidence.
Narrow‑Bore Column for Increased Resolution
The 2.1 mm ID × 100 mm length configuration provides:
Increased plate count compared to 50 mm narrow‑bore columns
Improved resolution for closely eluting compounds
Reduced solvent consumption relative to 4.6 mm ID columns
This format is one of the most commonly selected sizes for LC‑MS workflows where resolution and sensitivity are both critical.
LC‑MS and UV Detection Considerations
This column is fully compatible with LC‑MS workflows. Because of the 2.1 mm internal diameter, optimal performance requires:
An HPLC or UHPLC system capable of stable, precise low‑flow delivery
Minimized system dead volume
Low‑volume flow cells when using UV detection
Systems lacking accurate low‑flow capability may not be suitable.
Operating Guidelines and System Requirements
Typical flow‑rate range: ~0.2–0.3 mL/min (method‑dependent)
System compatibility: HPLC or UHPLC systems capable of reliable low‑flow operation
Special requirements: Low‑volume detector flow cell recommended
Flow rate optimization should balance resolution, analysis time, and pressure limits.