Molnupiravir Analyzed with an Inverse Gradient in HPLC – AppNote

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ARTICLES, APPNOTES, FAQ (9296)
- AppNotes Library (1889)
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October 12, 2021
Tanya

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Pharmaceuticals / AppNotes TYPE-C Columns / ARTICLES, APPNOTES, FAQ

Oral Anti-Viral Medication – Aqueous Normal Phase Method

Molnupiravir can be analyzed using this simple “inverse gradient” HPLC Method shown below. This is offered as an orthogonal approach for a Reversed Phased Method and will show various polar impurities not detected by Reversed Phase HPLC.

Peak:
Molnupiravir

Method Conditions:
Column: Cogent Diamond Hydride™, 4µm, 100Å
Dimensions: 4.6 x 75mm
Mobile Phase: (95:5) Acetonitrile / DI Water with 0.1% Formic Acid
Injection Volume: 1µL
Flow Rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Molnupiravir is dissolved at a concentration of 0.5mg / mL in (50:50) DI Water / Acetonitrile

Note: Molnupiravir is an oral antiviral drug that was developed for the treatment of influenza. It is a prodrug of the synthetic nucleoside derivative N4-hydroxycytidine, and exerts its antiviral action through introduction of copying errors during viral RNA replication.

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