Category: HPLC Column Information
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Analyte retention decreases with mobile phase water content using the Cogent Bidentate C18 HPLC column has anything happened to the column - Tech Information
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Analyze phosphorylated compounds with the Cogent Diamond Hydride column - Tips & Suggestions
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Are there HPLC columns that could be used to separate both polar and nonpolar compounds in a single run - FAQ
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Assuring retention of polar compounds in an Aqueous Normal Phase ANP gradient in positive ion mode LCMS - Tips & Suggestions
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Biological extracts Diamond Hydride columns and LCMS ion source diminished peak height problem - FAQ
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Blocked HPLC column from proteins from the sample matrix can be recovered - Tips & Suggestions
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Can I expect a more predictable retention time with Silica-C columns than with ordinary silica - FAQ
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Can I use a silica or Silica-C guard column with an analytical column of a different phase - FAQ
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Can I use phosphoric acid or sodium phosphate with the Cogent Diamond Hydride HPLC column - FAQ
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Can I use the certified HPLC column supplied in the HSQ Kit for UHPLC performance qualification - FAQ
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Can I use the Cogent Diamond Hydride column without mobile phase additives like formic acid - FAQ
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Causes of high baseline noise with Cogent Diamond Hydride HPLC column - Tips & Suggestions
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Changing retention times of amino acids in biological extracts using ammonium acetate in HPLC methods. - Troubleshooting.
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Direct silicon to carbon bonds on Cogent TYPE-C HPLC columns produce longer column lifetimes
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Do I need any special fittings to use the Cogent Column Filter with my current HPLC column - FAQ
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Do Tanaka Plots for HPLC apply to columns used with Aqueous Normal Phase ANP methods - FAQ
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Does the Hichrom brand column coupler work with the Partisil and Partisphere HPLC columns - FAQ
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Eliminating shoulder peaks in an HPLC method using Cogent RP C18 columns - Tips & Suggestions
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For sugars analyzed by LCMS sodium acetate is recommended for sensitivity of Na adducts - Tips & Suggestions
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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Glucose pyruvate taurocholic acid gamma glutamylcysteine and other analysis - Tips & Suggestions
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High molecular weight compounds that won't retain on Cogent Diamond Hydride column try Cogent Bidentate C8 300 - Tips & Suggestions
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High pressure develops after flushing a Cogent Diamond Hydride column with acetonitrile cause and solution - FAQ
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How can Cogent TYPE-C HPLC columns offer faster results compared to other type of silica based columns - FAQ
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How can I improve accuracy and precision when separating polar compounds by HPLC using Aqueous Normal Phase ANP - FAQ
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How can I restore my Diamond Hydride HPLC column after I ran out of mobile phase and air was accidently introduced into it - FAQ
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How do the terms nano capillary and micro LC differ in terms of column ID and typical flow rates - FAQ
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How does ammonium fluoride affect HPLC and LCMS data if using Aqueous Normal Phase ANP methods - FAQ
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How does the shorter column supplied in the HSQ kit affect the data compared to the longer column from the PQ Kit - FAQ
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How often do I need to do the conditioning procedure for a new Diamond Hydride column - FAQ
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How similar fittings for chromatography can differ from different brands in HPLC - Tech Information
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How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
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How to eliminate retention time drift in LCMS with Aqueous Normal Phase ANP methods - Tips & Suggestions
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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How to improve peak shapes, retention & carryover for polar compounds with the Cogent Diamond Hydride™ column - Tips & Suggestions
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How to keep the Cogent Diamond Hydride column clean when using blood as a sample - Tips & Suggestions
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I am not observing any peaks for succinic acid or alpha-ketoglutarate with the Cogent Diamond Hydride column - Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
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Injection Technique for Aqueous Samples using Cogent TYPE-C Columns in ANP - Tips & Suggestions
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Issues arising from changes in mobile phase with acids to one with ammonium salts. Tips & Suggestions
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Lower retention is observed when functional group in a compound is ionized - Tips & Suggestions
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Metal free stainless steel HPLC columns comparison to PEEK column performance - Tips & Suggestions
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Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
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Modify selectivity in an ANP method of HPLC using different mobile phase pH - Tips & Suggestions
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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Polar and non polar peptides analyzed by LCMS with an Aqueous Normal Phase ANP method - Tips & Suggestions
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Protecting HPLC analytical columns and when to use guard columns vs Cogent Column Filters - Tips & Suggestions
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Restoring the initial results with a method for analyzing AMP ADP ATP UDP and GPT in biological extracts - Tips & Suggestions
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Solvents and solutions that should be avoided when using PEEK filters fittings or tubing - Tech Information
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Solving baseline problems in ANP methods with the Cogent Diamond Hydride column - Tips & Suggestions
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Suggestion for separation of phenylephrine HCl acetaminophen and dextromethorphan - Tips & Suggestions
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Troubleshooting problems with poor HPLC runs before the examining the column - Tips & Suggestions
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Understanding some of the differences between HILIC columns and TYPE-C columns - Tips & Suggestions
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Using methanol instead of acetonitrile in an HPLC method should be done with caution - Tips & Suggestions
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Using temperature changes for separations of terpenoids with the Cogent Bidentate C18 and UDC-Cholesterol HPLC columns - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies - FAQ
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What are some of the advantages of using Cogent TYPE-C prep and semi-prep HPLC columns - FAQ
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What are the main differences between Cogent Amide columns and other brands of Amide - FAQ
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What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
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What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
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What is the bonded phase of the Cogent PFP HPLC column and for which applications is it used - FAQ
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What is the chemical structure of the ligand in the Cogent DIOL HPLC stationary phase - FAQ
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What is the compatibility of Cogent TYPE-C Silica HPLC columns with UHPLC instruments - FAQ
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What is the difference between ferrules made of stainless steel vs stainless steel and PEEK hybrids - FAQ
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What is the difference between weak and strong cation exchange for Ion Exchange columns - FAQ
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What is the maximum loading capacity mass injection volume that can be used for a semi prep Cogent TYPE-C HPLC column - FAQ
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What is the most effective way to clean HPLC columns after or before use - Tips & Suggestions
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What is the pH range of 316 stainless steel laboratory tubing fittings and sparging stones - FAQ
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What is the pilot length or dimension X depth for end fittings uses to connect HPLC columns - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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What pressure should I expect with a Diamond Hydride 2.o HPLC column at flow rates from 0.2 to 2.0mL / min - FAQ
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What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time - FAQ
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What type of tubing do I use to connect the guard column holder to analytical columns - FAQ
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What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column - FAQ
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Which Cogent TYPE-C column do you recommend for the separation of positional isomers by HPLC or LCMS - FAQ
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Which column do you suggest for Cocaine & metabolite Ecognin separation - Tips & Suggestions
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Which HPLC column ID dimensions are the Hichrom brand guard column holders cat no 82000-GH compatible with - FAQ
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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Why do I have lower than normal back pressure with Cogent Diamond Hydride HPLC columns - FAQ
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Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column - FAQ
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Why you should use temperature control with the UDC-Cholesterol column - Tips & Suggestions
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