Category: HPLC Column Information
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A white film developed on my MS source so is this column packing material - FAQ
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Adjusted retention time in HPLC - HPLC Primer
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Advantages of using a 3.0mm ID HPLC column for LCMS - Tech Information
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Analyte retention decreases with mobile phase water content using the Cogent Bidentate C18 HPLC column has anything happened to the column - Tech Information
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Analyze phosphorylated compounds with the Cogent Diamond Hydride column - Tips & Suggestions
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Aqueous Normal Phase ANP and how can it is useful for polar compounds - Tech Information
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Aqueous Normal Phase ANP HPLC what is it - FAQ
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Aqueous Normal Phase ANP solvent strength order - FAQ
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Are 10um pore size available in the Cogent Column Filters - FAQ
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Are Cogent 2.o HPLC columns compatible with Agilent 1290 Infinity II instruments - FAQ
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Are Cogent TYPE-C silica 2.o columns considered UHPLC phases - FAQ
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Are the Soft-Guard septa and caps compatible with LCMS or LCMS / MS - FAQ
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Are there HPLC columns that could be used to separate both polar and nonpolar compounds in a single run - FAQ
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Assuring retention of polar compounds in an Aqueous Normal Phase ANP gradient in positive ion mode LCMS - Tips & Suggestions
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Better methods than HILIC in HPLC and LCMS - Tips & Suggestions
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Biological extracts Diamond Hydride columns and LCMS ion source diminished peak height problem - FAQ
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Blocked HPLC column from proteins from the sample matrix can be recovered - Tips & Suggestions
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Bubbling or fizzing coming from a new Cogent TYPE-C column - Tech Information
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Calculate assay of lisinopril dihydrate based on the anhydrous form - How to
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Calculate the ligand density of a bonded phase of an HPLC column - How To
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Can Aqueous Normal Phase ANP methods be used to separate steroids - FAQ
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Can Cogent TYPE-C HPLC columns be used in supercritical fluid chromatography SFC - FAQ
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Can I backflush the Cogent column filter for longer in-service life - FAQ
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Can I change the filter element in the Cogent Column Filters - FAQ
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Can I expect a more predictable retention time with Silica-C columns than with ordinary silica - FAQ
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Can I use a silica or Silica-C guard column with an analytical column of a different phase - FAQ
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Can I use Acetone as a mobile phase solvent in Aqueous Normal Phase ANP - FAQ
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Can I use ion pairing reagents with the Cogent Diamond Hydride HPLC column - FAQ
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Can I use of the Cogent Diamond Hydride HPLC column above pH 7 in HPLC - FAQ
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Can I use phosphoric acid or sodium phosphate with the Cogent Diamond Hydride HPLC column - FAQ
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Can I use Silica C columns in a Normal Phase gradient method - FAQ
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Can I use the certified HPLC column supplied in the HSQ Kit for UHPLC performance qualification - FAQ
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Can I use the Cogent Diamond Hydride column without mobile phase additives like formic acid - FAQ
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Can I use the HSQ Kit with the longer column from the original PQ Kit - FAQ
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Can I use triethylamine TEA with a Cogent Silica-C column - FAQ
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Can I use triethylamine TEA with Cogent Diamond Hydride columns - FAQ
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Can rapid changes in column back pressure damage an HPLC column - FAQ
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Can the Cogent Column Filters replace my HPLC guard columns - FAQ
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Causes of an HPLC column appearing to lack sensitivity in LCMS - Tips & Suggestions
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Causes of high baseline noise with Cogent Diamond Hydride HPLC column - Tips & Suggestions
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Changing column ID means changing your gradient - Tips & Suggestions
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Changing retention times of amino acids in biological extracts using ammonium acetate in HPLC methods. - Troubleshooting.
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Choose an appropriate guard column when using a Cogent TYPE-C HPLC column - How To
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Choose an appropriate re-equilibration or post time for gradient HPLC - How To
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Choosing the correct HPLC column coupler - How To
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Cleaning Cogent TYPE-C silica based HPLC columns - Tips & Suggestions
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Cogent amino HPLC columns designations for reversed or normal phase - Tech Information
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Cogent Cyano RP and Cyano NP definitions - INTERNAL
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Cogent silica hydride HPLC columns by order of hydrophobicity - Tips & Suggestions
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Cogent TYPE-C column end fitting hardware and dimensions - Tips & Suggestions
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Column cleaning and care in UHPLC columns - Tips & Suggestions
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Common cause of excessive back pressure in HPLC columns - FAQ
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Converting Cogent TYPE-C columns to normal phase after use in reversed phase - How To
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Determine pore volume of an HPLC column using HPLC injections - How To
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Determine the asymmetry factor in HPLC at 10 percent peak height - How To
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Determining efficiency of HPLC methods 4um v 2um - FAQ
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Developing a Bromhexine HPLC method - Tips & Suggestions
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Direct silicon to carbon bonds on Cogent TYPE-C HPLC columns produce longer column lifetimes
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Do all PEEK HPLC fittings fit all receiving ports - FAQ
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Do I need a connector for cartridge columns that use Partisphere WVS hardware - FAQ
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Do I need any special fittings to use the Cogent Column Filter with my current HPLC column - FAQ
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Do Tanaka Plots for HPLC apply to columns used with Aqueous Normal Phase ANP methods - FAQ
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Do the Cogent Silica-C columns contain any carbon - FAQ
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Do you offer a 0.5um titanium or PEEK frit for the PEEK column hardware - FAQ
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Does the Hichrom brand column coupler work with the Partisil and Partisphere HPLC columns - FAQ
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Eliminate high background noise using a new Cogent HPLC column - Tips & Suggestions
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Eliminating shoulder peaks in an HPLC method using Cogent RP C18 columns - Tips & Suggestions
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Ferrule selection suggestions - Tech Information
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For sugars analyzed by LCMS sodium acetate is recommended for sensitivity of Na adducts - Tips & Suggestions
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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Fosetyl Aluminum analysis by HPLC - Tips & Suggestions
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Fosetyl aluminum analysis by Reversed Phase HPLC - Tips & Suggestions
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Fractions to decimals to metric chart - Tech Information
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Glucose pyruvate taurocholic acid gamma glutamylcysteine and other analysis - Tips & Suggestions
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Guidelines for packing in the PEEK column hardware - Tips & Suggestions
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High molecular weight compounds that won't retain on Cogent Diamond Hydride column try Cogent Bidentate C8 300 - Tips & Suggestions
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High pressure develops after flushing a Cogent Diamond Hydride column with acetonitrile cause and solution - FAQ
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How a pulse from an HPLC pump affect the column and its performance - Tech Information
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How can Cogent TYPE-C HPLC columns offer faster results compared to other type of silica based columns - FAQ
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How can I get separation of tartaric and malic acid in a complex mixture? - FAQ.
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How can I improve accuracy and precision when separating polar compounds by HPLC using Aqueous Normal Phase ANP - FAQ
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How can I restore my Diamond Hydride HPLC column after I ran out of mobile phase and air was accidently introduced into it - FAQ
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How can I separate capsaicinoids in hot sauce by HPLC - Tips & Suggestions
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How can I separate methacrylic acid and dodecyl benzene sulfonic acid - FAQ
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How do I clean the PQ or HSQ kit HPLC column - FAQ
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How do I connect a guard column to an analytical column - FAQ
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How do I stack multiple ColumnStore column storage cabinet units - FAQ
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How do the terms nano capillary and micro LC differ in terms of column ID and typical flow rates - FAQ
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How does ammonium fluoride affect HPLC and LCMS data if using Aqueous Normal Phase ANP methods - FAQ
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How does efficiency in Aqueous Normal Phase ANP compare to HILIC in HPLC - FAQ
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How does the Cogent UDA HPLC column's ligand bond to the silica hydride surface - FAQ
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How does the shorter column supplied in the HSQ kit affect the data compared to the longer column from the PQ Kit - FAQ
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How many injections should I expect on the HSQ Kit HPLC column - FAQ
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How often do I need to clean or wash a Cogent TYPE-C column to keep data precise - FAQ
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How often do I need to do the conditioning procedure for a new Diamond Hydride column - FAQ
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How similar fittings for chromatography can differ from different brands in HPLC - Tech Information
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How to back flush and wash an HPLC column for reuse - Tips & Suggestions
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How to condition Cogent RP or HPS or Axis HPLC columns for storage - Tips & Suggestions
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How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
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How to eliminate retention time drift in LCMS with Aqueous Normal Phase ANP methods - Tips & Suggestions
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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How to improve LOD or detection limits in HPLC - Tips & Suggestions
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How to improve peak shapes, retention & carryover for polar compounds with the Cogent Diamond Hydride™ column - Tips & Suggestions
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How to keep HPLC columns free of adsorbed contaminants - How To
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How to keep the Cogent Diamond Hydride column clean when using blood as a sample - Tips & Suggestions
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How to separate phospholipids with HPLC - Tips & Suggestions
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HPLC stationary phase can be responsible for a blocked frit- Tips & Suggestions
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I am not observing any peaks for succinic acid or alpha-ketoglutarate with the Cogent Diamond Hydride column - Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
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If I do not remove gas from my mobile phase solvents what are the consequences - FAQ
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Improvement in signal to noise in LCMS with an ANP method - Tips & Suggestions
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Increasing selectivity and resolution for a xylose and ribose method - Tips & Suggestions
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Injection Technique for Aqueous Samples using Cogent TYPE-C Columns in ANP - Tips & Suggestions
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Is the Cogent Column Filter compatible with a 2.1mm ID column - FAQ
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Is the Cogent UDC Cholesterol HPLC column stable as a stationary phase - FAQ
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Issues arising from changes in mobile phase with acids to one with ammonium salts. Tips & Suggestions
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Issues with a steep UV trace slope in gradient HPLC methods - Tips & Suggestions
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Know the void volume of an HPLC column - Tips & Suggestions
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Literature references for Cogent TYPE-C HPLC columns - Tech Information
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Lower retention is observed when functional group in a compound is ionized - Tips & Suggestions
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Measure signal to noise ratio in an HPLC chromatogram - How to
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Metal free stainless steel HPLC columns comparison to PEEK column performance - Tips & Suggestions
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Method development tip for maleic hydrazide - Tips & Suggestions
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Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
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Modify selectivity in an ANP method of HPLC using different mobile phase pH - Tips & Suggestions
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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NMR structure confirmation data for Cogent Bidentate C18 phases - Tech Information
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NMR structure confirmation data for Cogent Bidentate C8 phases - Tech Information
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NMR structure confirmation data for Cogent UDC-cholesterol phases - Tech Information
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Octanol Water Partition coefficient - Primer
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Peak area decreases with increasing flow rate - FAQ
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PEEK column hardware end fitting dimensions - INTERNAL ONLY
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PEEK slurry reservoir 2.1mm ID column packing hardware information - FAQ
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Percentage of acid needed in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Polar and non polar peptides analyzed by LCMS with an Aqueous Normal Phase ANP method - Tips & Suggestions
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Policy on verifying a new Cogent HPLC column performance - Tips & Suggestions
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Poor HPLC peak shape can be due to injection sample mismatch - Tips & Suggestions
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Poor peak shape for ethylenediamine in LCMS analyses - Tech Information
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Protecting HPLC analytical columns and when to use guard columns vs Cogent Column Filters - Tips & Suggestions
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Restoring the initial results with a method for analyzing AMP ADP ATP UDP and GPT in biological extracts - Tips & Suggestions
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Ribose and Xylose analysis without forming Schiff bases - AppNote
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Sample concentration changes can effect retention time in HPLC - Primer
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Solvents and solutions that should be avoided when using PEEK filters fittings or tubing - Tech Information
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Solving baseline problems in ANP methods with the Cogent Diamond Hydride column - Tips & Suggestions
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Solving baseline problems in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Specifications of different grades of stainless steel used in HPLC columns
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Store and condition Cogent TYPE-C HPLC columns - How To
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Store and maintain Cogent Diamond Hydride HPLC columns - How To
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Storing the HPLC column supplied in the HSQ kit - How To
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Suggestion for separation of phenylephrine HCl acetaminophen and dextromethorphan - Tips & Suggestions
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Suggestions for HPLC method development with silica hydride columns - Tips & Suggestions
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Suggestions on how to separate capsaicinoids in hot sauce by HPLC - Tips & Suggestions
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System suitability requirements for a USP HPLC method - Tips & Suggestions
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The pH range of PEEK laboratory tubing and fittings- Tech Information
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Theoretical plate estimates indicates column condition - Primer
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Theoretical plate N estimate for real samples - How to
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Troubleshooting problems with poor HPLC runs before the examining the column - Tips & Suggestions
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Types of isomers and suitable HPLC columns for separation - Tips & Suggestions
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Understanding some of the differences between HILIC columns and TYPE-C columns - Tips & Suggestions
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Use a Cogent TYPE-C column to develop new methods - Tips & Suggestions
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Using Aqueous Normal Phase ANP to your advantage - White Paper
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Using inorganic buffers for HPLC UV analysis of citric acid - Tips & Suggestions
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Using methanol instead of acetonitrile in an HPLC method should be done with caution - Tips & Suggestions
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Using temperature changes for separations of terpenoids with the Cogent Bidentate C18 and UDC-Cholesterol HPLC columns - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What 316 in 316 stainless steel means - Primer
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What are Cogent HPS HPLC Columns - FAQ
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What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies - FAQ
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What are some of the advantages of using Cogent TYPE-C prep and semi-prep HPLC columns - FAQ
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What are the dimensions of 4.6mm ID 5um frits for columns made with PEEK - FAQ
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What are the main differences between Cogent Amide columns and other brands of Amide - FAQ
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What are the maximum changes allowed in a USP method- FAQ
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What are the metal contents of the silica used in Cogent TYPE-C HPLC columns - FAQ
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What are the metals content in Cogent HPS silica - FAQ
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What are the pH ranges of common buffers for HPLC - Tips & Suggestions
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What are the thread specifications for the PEEK columns - FAQ
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What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
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What are the uses of PEEK HPLC column hardware - FAQ
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What are theoretical plates and HETP in HPLC - Primer
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What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
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What column changes are permitted in USP HPLC methods - FAQ
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What do You suggest for an internal standard in glucosamine LCMS Analysis - FAQ
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What does stainless in stainless steel mean - FAQ
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What does the term 10-32 mean to a chromatographer working with HPLC vials - FAQ
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What does the term Activity refer to in chromatography - FAQ
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What is a base deactivated HPLC Column - FAQ
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What is a master batch for Cogent HPLC columns - FAQ
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What is the biggest difference between traditional HPLC phases and Bidentate C18 - FAQ
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What is the bonded phase of the Cogent PFP HPLC column and for which applications is it used - FAQ
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What is the Bubbling or Fizzing Coming from a New Cogent TYPE-C Column? - FAQ
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What is the carbon load of the Cogent Diamond Hydride HPLC column - FAQ
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What is the cause of a pressure increase after injections of blanks in HPLC - FAQ
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What is the chemical structure of the ligand in the Cogent DIOL HPLC stationary phase - FAQ
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What is the column to column reproducibility for Cogent HPS columns - INTERNAL ONLY
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What is the compatibility of Cogent TYPE-C Silica HPLC columns with UHPLC instruments - FAQ
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What is the definition of a fitting for HPLC - FAQ
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What is the definition of passivating and purging in HPLC - FAQ
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What is the difference between a 4um and a 5um particle in a C18 column - FAQ
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What is the difference between a low and high pressure union in HPLC - FAQ
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What is the difference between a union and an adapter in HPLC - FAQ
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What is the difference between a Waters type fitting and a standard 10-32 fitting - FAQ
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What is the difference between ferrules made of stainless steel vs stainless steel and PEEK hybrids - FAQ
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What is the difference between the ColumnStore 120 and four ColumnStore 30 - FAQ
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What is the difference between weak and strong cation exchange for Ion Exchange columns - FAQ
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What is the largest Internal Diameter for Cogent TYPE-C prep columns - FAQ
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What is the maximum loading capacity mass injection volume that can be used for a semi prep Cogent TYPE-C HPLC column - FAQ
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What is the maximum pressure Cogent HPLC columns can tolerate - FAQ
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What is the maximum pressure of the 2.1mm PEEK HPLC columns - FAQ
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What is the maximum pressure of your guard columns - FAQ
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What is the maximum pressure rating for Cogent HPLC columns? - FAQ
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What is the maximum pressure the Cogent Column Filters should be used with - FAQ
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What is the maximum temperature for Cogent Diamond Hydride HPLC columns - FAQ
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What is the most effective way to clean HPLC columns after or before use - Tips & Suggestions
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What is the pH range of 316 stainless steel laboratory tubing fittings and sparging stones - FAQ
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What is the pH stability of Cogent HPS HPLC columns - FAQ
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What is the pilot length or dimension X depth for end fittings uses to connect HPLC columns - FAQ
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What is the pressure capability of the ARE brand all PEEK column coupler - FAQ
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What is the pressure rating of the Hichrom brand column coupler - FAQ
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What is the purpose of back flushing an HPLC column and will it damage it - FAQ
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What is the purpose of different bonded phases using Cogent TYPE-C Silica - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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What is the recommended pH range for Cogent Diamond Hydride HPLC columns - FAQ
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What is the structure of the Cogent Phenyl Hydride bonded ligand - FAQ
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What is the thickness of the SS filter element in the Cogent Column Filters - FAQ
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What is the useful temperature range of PEEK laboratory products - FAQ
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What is the volume of Cogent HPLC columns - FAQ
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What materials are the Cogent Column Filters manufactured with - FAQ
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What pressure should I expect with a Diamond Hydride 2.o HPLC column at flow rates from 0.2 to 2.0mL / min - FAQ
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What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time - FAQ
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What results when I use a small guard column with a larger ID or prep column - FAQ
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What type of fitting is best to use with the Cogent HPLC and LCMS columns - FAQ
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What type of tubing do I use to connect the guard column holder to analytical columns - FAQ
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What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column - FAQ
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What would you recommend to reduce time to develop an HPLC method - Tips & Suggestions
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When is the acid group of the Cogent UDA HPLC column ionized - Tech Information
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When to use PEEK or stainless steel for HPLC column hardware - Tips & Suggestions
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Which Cogent TYPE-C column do you recommend for the separation of positional isomers by HPLC or LCMS - FAQ
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Which column do you suggest for Cocaine & metabolite Ecognin separation - Tips & Suggestions
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Which columns are compatible with the Hichrom brand column coupler Cat No 47450-06 - FAQ
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Which ferrules are best when using soft polymeric tubing - FAQ
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Which HPLC column do you suggest for analysis of carotene or lycopene - FAQ
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Which HPLC column ID dimensions are the Hichrom brand guard column holders cat no 82000-GH compatible with - FAQ
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Which HPLC column internal diameter should I use for LCMS - FAQ
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Which membrane filter disc is best to use with aqueous buffers - FAQ
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Which size guard column should I use with my 1mm Cogent 2.o column - FAQ
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Which solvents should not be used with the Cogent Column Filters - FAQ
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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Why am I seeing two peaks for quaternium15 Dowicil-75 in LCMS - FAQ
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Why am I seeing two peaks for quaternium-15 or Dowicil-75 in my LCMS data - FAQ
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Why are Aqueous Normal Phase ANP HPLC methods better to use than HILIC - FAQ
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Why are the Cogent Silica-C columns more stable than other silica columns - FAQ
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Why do I have higher pressure at a lower flow rate in my HPLC runs - FAQ
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Why do I have lower than normal back pressure with Cogent Diamond Hydride HPLC columns - FAQ
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Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column - FAQ
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Why do some HPLC methods use low pH buffers to separate acidic compounds - FAQ
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Why do the PEEK columns not have wrench flats on them - FAQ
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Why use buffers during analysis and acid buffers such as the phosphoric acid for column storage solvents for Bidenate C18 columns? Will phosphate precipitate in the column INTERNAL ONLY
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Why would I want to use an HPLC column that is not end capped - FAQ
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Why you should use temperature control with the UDC-Cholesterol column - Tips & Suggestions
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Will I protect HPLC columns from samples that get permanently adsorbed to the column by using a syringe filter - FAQ
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Will using the Cogent Column Filters change my current HPLC results - FAQ
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Working with ammonium acetate and LCMS requires attention to cleaning - Tips & Suggestions
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