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Category: HPLC Suggestions and Information
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HPLC Suggestions and Information
Cogent TYPE-C columns used in prep chromatography offer value - Tips & Suggestions
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Column ID Considerations for LCMS Applications - Tech Information
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Convert HPLC columns between Reversed Phase and Normal Phase - How to
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Cut PEEK tubing using a Clean Cut90 cutter - How to
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Cut stainless steel tubing for HPLC properly - How to
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Determining HPLC method accuracy - How To
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Determining the precision of an HPLC method - Tips & Suggestions
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Developing a Bromhexine HPLC method - Tips & Suggestions
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Do Autosampler Vials and Caps Have a Maximum Temperature Range - FAQ
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Do the Cogent Silica-C columns contain any carbon - FAQ
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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Eliminate high background noise using a new Cogent HPLC column - Tips & Suggestions
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Eliminating shoulder peaks in an HPLC method using Cogent RP C18 columns - Tips & Suggestions
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Glucose pyruvate taurocholic acid gamma glutamylcysteine and other analysis - Tips & Suggestions
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Helpful HPLC columns you may not know about - White Paper
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High molecular weight compounds that won't retain on Cogent Diamond Hydride column try Cogent Bidentate C8 300 - Tips & Suggestions
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How can I fix split peaks with high back pressure in HPLC analyses - FAQ
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How does ammonium fluoride affect HPLC and LCMS data if using Aqueous Normal Phase ANP methods - FAQ
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How to back flush and wash an HPLC column for reuse - Tips & Suggestions
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How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
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How to condition Cogent RP or HPS or Axis HPLC columns for storage - Tips & Suggestions
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How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
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How to fix a saw tooth pattern in the UV baseline - Tips & Suggestions
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How to keep surface treated frits from biofouling - FAQ
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If I do not remove gas from my mobile phase solvents what are the consequences - FAQ
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If you are not seeing an expected peak in an HPLC assay - Tips & Suggestions
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Increasing selectivity and resolution for a xylose and ribose method - Tips & Suggestions
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Integrate a peak with a shoulder - How to
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Is the Cogent Column Filter compatible with a 2.1mm ID column - FAQ
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LFER studies compared to Log D data in describing the Aqueous Normal Phase ANP retention - Tips & Suggestions
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Lower retention is observed when functional group in a compound is ionized - Tips & Suggestions
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Measure signal to noise ratio in an HPLC chromatogram - How to
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Missing peaks using UV detection in HPLC methods - Tech Information
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Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
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Negative Baselines in HPLC at 214nm - Tips & Suggestions
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No need to use a high pH mobile phases for compounds with a high pKa - Tips & Suggestions
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Obtain a flat baseline in a gradient method without using blank subtraction - How to
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Phospholipid Separations by HPLC - Tips & Suggestions
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pK Values of common chemical functional groups in HPLC - PRIMER
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Polar and non polar peptides analyzed by LCMS with an Aqueous Normal Phase ANP method - Tips & Suggestions
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Precision in Analytical Analysis Definition Made Easy - PRIMER
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Purge metals from HPLC system using EDTA - How To
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Quaternary amines analysis by LCM and finding the right ion - How To
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Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run - Tips & Suggestions
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Solving baseline problems in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Store and condition Cogent TYPE-C HPLC columns - How To
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Store and maintain Cogent Diamond Hydride HPLC columns - How To
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System suitability Requirements for a USP HPLC Method - Tips & Suggestions
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Techniques to prepare a 0.05% solution from a tablet for HPLC injection - How to
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The differences in ion content between clear and amber borosilicate glass vials - Tech Information
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Theoretical plate estimates indicates column condition - Primer
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Tip for Promoting Analyte Ionization with LCMS – AppNote
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Two gradients to use for analysis of hydrophilic and or hydrophobic molecules in the same Aqueous Normal Phase ANP method - Tips & Suggestions
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Types of Chemical Isomers and Recommended HPLC Columns for Effective Separation - Tips & Suggestions
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Understanding some of the differences between HILIC columns and TYPE-C columns - Tips & Suggestions
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Use Teflon bottles for A and B solvents in negative ion mode LCMS - Primer
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Using an Aqueous Normal Phase ANP method will sodium ions interfere with succinate when analyzing sodium succinate - FAQ
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Using Aqueous Normal Phase ANP to your advantage - White Paper
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Using temperature changes for separations of terpenoids with the Cogent Bidentate C18 and UDC-Cholesterol HPLC columns - Tips & Suggestions
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Using triethylamine TEA in Aqueous Normal Phase ANP methods for simple sugars - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What are inosine nucleotides - FAQ
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What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies - FAQ
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What are the Advantages and disadvantages of Acid Washing RSA Autosampler Vials - FAQ
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What are the Differences Between Nylon and PTFE Syringe Filters for Chromatography - FAQ
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What are the pH ranges of common buffers for HPLC - Tips & Suggestions
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What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
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What column changes are permitted in USP HPLC methods - FAQ
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What effect does high injection volume have on column performance in Aqueous Normal Phase ANP HPLC- FAQ
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What is the best way to track samples in U-2D inserts - Tips and Suggestions
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What is the difference between a 4um and a 5um particle in a C18 column - FAQ
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What is the maximum concentration of ammonium fluoride to use in a mobile phase to avoid column damage - Tips & Suggestions
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What is the most effective way to clean HPLC columns after or before use - Tips & Suggestions
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Which HPLC column do you suggest for analysis of carotene or lycopene - FAQ
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Which is better to Use Snap Top or Screw Top Autosampler Vials and Caps for Preventing Evaporation - FAQ
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Which size guard column should I use with my 1mm Cogent 2.o column - FAQ
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Why am I seeing multiple peaks in HPLC runs of fructose - Tips & Suggestions
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Why are the Cogent Silica-C columns more stable than other silica columns - FAQ
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Why is isopropanol rarely used as a solvent in HPLC or LCMS methods - FAQ
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Why would I want to use an HPLC column that is not end capped - FAQ
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Will a different guard column hardware system different from the Cogent system affect my results when using a Cogent column - FAQ
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Will TFA permanently alter retention / selectivity of the Cogent TYPE-C silica columns - FAQ
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