Category: HPLC Suggestions and Information
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A white film developed on my MS source so is this column packing material - FAQ
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Acceptable wavelength band width for HPLC detectors when performing an HPLC Performance Qualification - Tech Information
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Achieve optimal seals when crimping caps on vials - How to
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Add 10 micro molar EDTA or medronic acid to mobile phase for better peak shapes - Tips & Suggestions
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Adjusted retention time hold up time - Primer
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Adjusted retention time in HPLC - HPLC Primer
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Adsorption can happen quickly in an autosampler vial - Tips & Suggestions
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Advantages of using a 3.0mm ID HPLC column for LCMS - Tech Information
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Analyte retention decreases with mobile phase water content using the Cogent Bidentate C18 HPLC column has anything happened to the column - Tech Information
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Analyze phosphorylated compounds with the Cogent Diamond Hydride column - Tips & Suggestions
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AQ brand polypropylene MS vials compared to market leading plastic vials for LCMS - FAQ
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Aqueous Normal Phase ANP and how can it is useful for polar compounds - Tech Information
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Aqueous Normal Phase ANP ballistic gradients compared to same in HILIC - Tips & Suggestions
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Aqueous Normal Phase ANP defined & compared to Reversed Phase & Normal Phase definitions HPLC
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Aqueous Normal Phase ANP HPLC retention mechanism elucidated - Tech Information
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Aqueous Normal Phase ANP HPLC what is it - FAQ
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Aqueous Normal Phase ANP solvent strength order - FAQ
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Are RSA vials suitable for elemental analysis - FAQ
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Are the AQ Advanced Quality brand of LCMS vials compatible with DMSO - FAQ
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Are the Chemical Solutions qualification solutions for HPLC NIST traceable - FAQ
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Are there HPLC columns that could be used to separate both polar and nonpolar compounds in a single run - FAQ
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Basic compound adsorption study with time dependence in vials - AppNote
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Basic compound functional group dependent adsorption study in RSA autosampler vials - AppNote
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Better methods than HILIC in HPLC and LCMS - Tips & Suggestions
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Better separation of two peaks for Reversed Phase RP HPLC - Tips & Suggestions
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Biological extracts Diamond Hydride columns and LCMS ion source diminished peak height problem - FAQ
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Bubbling or fizzing coming from a new Cogent TYPE-C column - Tech Information
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Buffer concentration can affect ANP retention of acids in HPLC Analyses - Tips & Suggestions
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Calculate assay of lisinopril dihydrate based on the anhydrous form - How to
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Calculating theoretical plates N at half height - How To
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Can Aqueous Normal Phase ANP methods be used to separate steroids - FAQ
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Can Cogent TYPE-C HPLC columns be used in supercritical fluid chromatography SFC - FAQ
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Can I backflush the Cogent column filter for longer in-service life - FAQ
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Can I make a PTFE syringe filter more hydrophilic - FAQ
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Can I use benzene as a mobile phase solvent in HPLC - FAQ
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Can I use the certified HPLC column supplied in the HSQ Kit for UHPLC performance qualification - FAQ
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Can I use the UV-vis Calibration Kit to qualify detection at 200nm - FAQ
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Can I use trifluoroacetic acid TFA to improve the chromatographic performance in LCMS - FAQ
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Can the all silicone cap mats be sterilized - FAQ
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Can the Cogent Column Filters replace my HPLC guard columns - FAQ
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Can the HSQ Kit be used to measure the LOQ and LOD differences between instruments - FAQ
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Can the HSQ or PQ qualification kits be used with Waters equipment and Empower chromatography data software - FAQ
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Can the U-2D Micro-Sample management system be used in SPE manifold for 96-well plates - FAQ
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Changing column ID means changing your gradient - Tips & Suggestions
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Check for high pressure traced to the HPLC pump module - Tips & Suggestions
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Choose an appropriate re-equilibration or post time for gradient HPLC - How To
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Cogent Cyano RP and Cyano NP definitions - INTERNAL
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Cogent silica hydride HPLC columns by order of hydrophobicity - Tips & Suggestions
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Cogent TYPE-C column end fitting hardware and dimensions - Tips & Suggestions
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Cogent TYPE-C columns used in prep chromatography offer value - Tips & Suggestions
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Compatibility guidelines for safety coated mobile phase reservoir bottle use - Tips & Suggestions
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Convert HPLC columns between Reversed Phase and Normal Phase - How to
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Converting Cogent TYPE-C columns to normal phase after use in reversed phase - How To
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Cut PEEK tubing using a Clean Cut90 cutter - How to
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Cut stainless steel tubing for HPLC properly - How to
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Cysteine analysis tips for LCMS - Tips & Suggestions
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Determine dwell volume of gradient HPLC systems - How To
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Determine the asymmetry factor in HPLC at 10 percent peak height - How To
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Determining efficiency of HPLC methods 4um v 2um - FAQ
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Determining HPLC method accuracy - How To
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Determining the precision of an HPLC method - Tips & Suggestions
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Determining wavelength accuracy below 230nm for HPLC instruments - How To
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Developing a Bromhexine HPLC method - Tips & Suggestions
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Difference between adsorption and absorption - FAQ
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Difficulties with wavelength accuracy readings while performing a PQ of an HPLC - Tips & Suggestions
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Do all PEEK HPLC fittings fit all receiving ports - FAQ
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Do autosampler vials and caps have a temperature range - FAQ
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Do Tanaka Plots for HPLC apply to columns used with Aqueous Normal Phase ANP methods - FAQ
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Do the Cogent Silica-C columns contain any carbon - FAQ
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Do you have suggestions for a knitted reactor coils KRC with a volume of 0.375ml with an internal diameter of 0.5mm - FAQ
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Does the method used in the HSQ Kit for HPLC need to be validated - FAQ
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Does the UV-vis calibration kit operate in all three ranges UV and visible and visible/NIR - FAQ
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Dwell volume effect on HPLC analyte retention in gradient methods - Primer
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Effect of diluent on analyte adsorption to glass autosampler vials - White Paper
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Effects of washing and re-using autosampler vials on sample loss - Tech Information
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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Eliminate high background noise using a new Cogent HPLC column - Tips & Suggestions
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Eliminating shoulder peaks in an HPLC method using Cogent RP C18 columns - Tips & Suggestions
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Establishing HPLC method linearity - Tips & Suggestions
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Features and benefits of the U-2D Micro Sample Management System - Tech Information
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For Normal Phase HPLC which syringe filter do you recommend - FAQ
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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Fractions to decimals to metric chart - Tech Information
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Ghost peaks appearing in results using a gradient with TFA which can degrade with time - Tips & Suggestions
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Glucose pyruvate taurocholic acid gamma glutamylcysteine and other analysis - Tips & Suggestions
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Has there been a risk assessment for the use of SOLVFil in GMP laboratories - FAQ
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Headspace septa temperature performance specifications - Tech Information
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Helpful HPLC columns you may not know about - White Paper
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High molecular weight compounds that won't retain on Cogent Diamond Hydride column try Cogent Bidentate C8 300 - Tips & Suggestions
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How a pulse from an HPLC pump affect the column and its performance - Tech Information
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How are RSA vials different from all other autosampler vials - FAQ
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How can a finger tight fitting be producing band broadening in HPLC - FAQ
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How can Cogent TYPE-C HPLC columns offer faster results compared to other type of silica based columns - FAQ
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How can I fix split peaks with high back pressure in HPLC analyses - FAQ
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How can I improve accuracy and precision when separating polar compounds by HPLC using Aqueous Normal Phase ANP - FAQ
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How can I make changes to locked cells in the Excel spreadsheet that comes with the HSQ qualification kit - FAQ
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How can I minimize bubble formation in limited volume vials when filling - FAQ
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How can I separate capsaicinoids in hot sauce by HPLC - Tips & Suggestions
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How can sample concentration have an effect on peak shape in HPLC - FAQ
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How does ammonium fluoride affect HPLC and LCMS data if using Aqueous Normal Phase ANP methods - FAQ
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How is pressure inside tubing calculated for HPLC - FAQ
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How long will it take for chloroform to degrade the plastic safety coating on the mobile phase bottles if the bottle is broken during a fall - FAQ
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How much pressure can the MicroSolv mobile phase bottles withstand without bursting - FAQ
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How often do I need to clean or wash a Cogent TYPE-C column to keep data precise - FAQ
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How often do I need to do the conditioning procedure for a new Diamond Hydride column - FAQ
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How tight should an autosampler vial cap be to prevent push through of the septa during injection - FAQ
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How to back flush and wash an HPLC column for reuse - Tips & Suggestions
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How to calibrate UV-vis and HPLC detectors with NIST traceable solutions below 230nm - Tips & Suggestions
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How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
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How to condition Cogent RP or HPS or Axis HPLC columns for storage - Tips & Suggestions
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How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
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How to fix a saw tooth pattern in the UV baseline - Tips & Suggestions
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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How to improve LOD or detection limits in HPLC - Tips & Suggestions
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How to increase HPLC UV detector response - Tips & Suggestions
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How to keep HPLC columns free of adsorbed contaminants - How To
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How to keep surface treated frits from biofouling - FAQ
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How to minimize HPLC column blockage from most common causes - Tips & Suggestions
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How to separate phospholipids with HPLC - Tips & Suggestions
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How to tighten screw caps properly - Tips & Suggestions
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HPLC Retention time shift troubleshooting - Tips & Suggestions
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HPLC stationary phase can be responsible for a blocked frit- Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
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I see extra peaks in my chromatography when I am not using the same filter - Tips & Suggestions
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If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
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If I do not remove gas from my mobile phase solvents what are the consequences - FAQ
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If you are not seeing an expected peak in an HPLC assay - Tips & Suggestions
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Improve or obtain flat baselines in HPLC methods with a gradient - Tips & Suggestions
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Improvement in signal to noise in LCMS with an ANP method - Tips & Suggestions
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Improving peak shapes in an HPLC method in aqueous normal phase ANP - Tips & Suggestions
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Increasing selectivity and resolution for a xylose and ribose method - Tips & Suggestions
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Integrate a peak with a shoulder - How to
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Interpreting mass spectral data - Primer
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Is PEEK tubing or PEEK fittings autoclavable - FAQ
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Is the Cogent Column Filter compatible with a 2.1mm ID column - FAQ
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Is the Excel template included in the Chemical Solutions Qualification kits CFR Part 21.11 compliant - FAQ
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Is the plastic used in the U-2D system autoclavable at 120°C - FAQ
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Is the software for the MicroSolv HPLC PQ Kit supplied on CD or can it be downloaded - FAQ
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Is there a difference in chromatography performance between clear and amber RSA vials - FAQ
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Is there any Bismuth in Soft-Guard™ Septa - FAQ
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Issues with a steep UV trace slope in gradient HPLC methods - Tips & Suggestions
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Know the void volume of an HPLC column - Tips & Suggestions
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Know when to discard an outlier data point in HPLC by using the Q Test - How To
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LFER studies compared to Log D data in describing the Aqueous Normal Phase ANP retention - Tips & Suggestions
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Lower retention is observed when functional group in a compound is ionized - Tips & Suggestions
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Measure signal to noise ratio in an HPLC chromatogram - How to
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Missing peaks using UV detection in HPLC methods - Tech Information
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Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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My retention times are changing after storing my buffer and what could be causing this and tips to help avoid issues - FAQ
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Negative Baselines in HPLC at 214nm - Tips & Suggestions
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No need to use a high pH mobile phases for compounds with a high pKa - Tips & Suggestions
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Obtain a flat baseline in a gradient method without using blank subtraction - How to
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Peak area decreases with increasing flow rate - FAQ
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Percentage of acid needed in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Performance goals or objectives for a routine HPLC method - Tips & Suggestions
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PFAS analysis using glass or plastic vials - Tips and Suggestions
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PFAS and other contaminants leaching into an analysis - Tips & Suggestions
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Polar and non polar peptides analyzed by LCMS with an Aqueous Normal Phase ANP method - Tips & Suggestions
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Poor HPLC peak shape can be due to injection sample mismatch - Tips & Suggestions
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Pre mix your diluent and mobile phase in sample preparation for HPLC - Tips & Suggestions
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Purge metals from HPLC system using EDTA or medronic acid - How To
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Quaternary amines analysis by LCM and finding the right ion - How To
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Random shifting of retention times in HPLC chromatograms - Tips & Suggestions
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Reasons for using direct adaptive Di-Ad HPLC column connectors - Tips & Suggestions
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Reference values for holmium oxide other than 1 nm – Tech Information
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Reliability of wet chemical assays versus HPLC assays - Primer
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Rescaling graphs in the MicroSolv HPLC PQ Kit Excel template - Tips & Suggestions
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Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run - Tips & Suggestions
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Ribose and Xylose analysis without forming Schiff bases - AppNote
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Sample concentration changes can effect retention time in HPLC - Primer
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Sample loop information - FAQ
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Screw and snap cap evaporation studies - Tech Information
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Sensitivity slowly diminishes in HPLC - Tips & Suggestions
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Should I filter my HPLC solvents and buffers before use - FAQ
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Should I use a 1 piece fitting or a 2 piece fitting for HPLC - FAQ
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Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP - FAQ
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ
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Solving baseline problems in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Spurious peaks from nylon syringe filters with DI water using electrochemical detection can be avoided - Tips & Suggestions
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Store and condition Cogent TYPE-C HPLC columns - How To
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Store and maintain Cogent Diamond Hydride HPLC columns - How To
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System suitability requirements for a USP HPLC method - Tips & Suggestions
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Techniques to prepare a 0.05% solution from a tablet for HPLC injection - How to
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Terminology and criteria for chirality - Primer
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The common names of HPLC column diameters - Primer
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The differences in ion content between clear and amber borosilicate glass vials - Tech Information
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The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase - Tips & Suggestions
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Theoretical plate estimates indicates column condition - Primer
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Tip for Promoting Analyte Ionization with LCMS – AppNote
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To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
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Two gradients to use for analysis of hydrophilic and or hydrophobic molecules in the same Aqueous Normal Phase ANP method - Tips & Suggestions
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Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run - Tips & Suggestions
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Types of isomers and suitable HPLC columns for separation - Tips & Suggestions
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Understanding some of the differences between HILIC columns and TYPE-C columns - Tips & Suggestions
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Use Teflon bottles for A and B solvents in negative ion mode LCMS - Primer
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Using an Aqueous Normal Phase ANP method will sodium ions interfere with succinate when analyzing sodium succinate - FAQ
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Using Aqueous Normal Phase ANP to your advantage - White Paper
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Using methanol instead of acetonitrile in an HPLC method should be done with caution - Tips & Suggestions
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Using temperature changes for separations of terpenoids with the Cogent Bidentate C18 and UDC-Cholesterol HPLC columns - Tips & Suggestions
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Using triethylamine TEA in Aqueous Normal Phase ANP methods for simple sugars - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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UV-vis instrument technology tips shared - Tech Information
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Validation protocol for the PQ kit template - Tips & Suggestions
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What are inosine nucleotides - FAQ
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What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies - FAQ
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What are some of the advantages of using Cogent TYPE-C prep and semi-prep HPLC columns - FAQ
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What are the advantages and disadvantages of acid washing RSA vials - FAQ
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What are the differences between 33 and 51 expansion glass and which should I choose - FAQ
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What are the differences between nylon and PTFE syringe filters for chromatography - FAQ
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What are the pH ranges of common buffers for HPLC - Tips & Suggestions
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What are the temperature and pressure specifications for stainless steel tubing - FAQ
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What are your definitions of normal phase reversed phase and ANP in HPLC - FAQ
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What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
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What can damage the surface treatment of silanized glass vials - FAQ
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What can I use for a reference solution against Holmium Oxide - FAQ
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What column changes are permitted in USP HPLC methods - FAQ
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What does analytical method validation entail for HPLC and LCMS - Primer
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What does residual volume mean with autosampler vials - FAQ
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What effect does high injection volume have on column performance in Aqueous Normal Phase ANP HPLC- FAQ
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What is a steady state for surfaces like syringe filters - FAQ
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What is a system peak as defined for HPLC - Primer
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What is considered an extractable that comes from syringe filters - FAQ
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What is peak inhibition in LCMS - FAQ
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What is pKa and how is it related to pH in HPLC mobile phases and sample diluents - FAQ
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What is Post Time in an HPLC gradient method - Primer
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What is TFA and when should I use it - Primer
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What is the best way to track samples in U-2D inserts - Tips and Suggestions
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What is the cause of a pressure increase after injections of blanks in HPLC - FAQ
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What is the compatibility of Cogent TYPE-C Silica HPLC columns with UHPLC instruments - FAQ
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What is the difference between a 4um and a 5um particle in a C18 column - FAQ
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What is the difference between AQ brand and low cost syringe filter brands - FAQ
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What is the difference between the terms 45 shore A and 55 shore A in septa - FAQ
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What is the difference between verifications validation and qualification - FAQ
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What is the durability of the SOLVFIL when used with standard mobile phases and buffered mobile phases - FAQ
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What is the effect of extra column volume for different HPLC instruments on the same method - FAQ
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What is the elution order for analytes in the Resolution Test Mixture supplied in the HSQ kit - Tech Information
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What is the largest Internal Diameter for Cogent TYPE-C prep columns - FAQ
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What is the maximum concentration of ammonium fluoride to use in a mobile phase to avoid column damage - Tips & Suggestions
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What is the maximum loading capacity mass injection volume that can be used for a semi prep Cogent TYPE-C HPLC column - FAQ
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What is the maximum pressure the Cogent Column Filters should be used with - FAQ
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What is the maximum salt concentration to use with Cogent TYPE-C HPLC columns with HPLC or LCMS - FAQ
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What is the mobile phase used in the HSQ Kit and how much is used - FAQ
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What is the most effective way to clean HPLC columns after or before use - Tips & Suggestions
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What is the pilot length or dimension X depth for end fittings uses to connect HPLC columns - FAQ
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What is the purpose of a partial slit septa - FAQ
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What is the purpose of a pre slit septa - FAQ
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What is the purpose of the Resolution Test Mixture supplied in your Qualification Kits - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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What is the useable volume in 2mL autosampler vials? - FAQ
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What makes glass borosilicate glass - Tech Information
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What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time - FAQ
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What type of fitting is best to use with the Cogent HPLC and LCMS columns - FAQ
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What type of tubing do I use to connect the guard column holder to analytical columns - FAQ
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What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column - FAQ
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When and why use Teflon bottles for your mobile phase reservoirs - FAQ
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When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative - Primer
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When is the acid group of the Cogent UDA HPLC column ionized - Tech Information
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When to use snap caps vs screw caps preventing evaporation - FAQ
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Where a static mixer for HPLC instruments should be placed - Tips & Suggestions
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Which HPLC column do you suggest for analysis of carotene or lycopene - FAQ
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Which HPLC column internal diameter should I use for LCMS - FAQ
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Which membrane filter disc is best to use with aqueous buffers - FAQ
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Which parameters can be determined with the supplied solutions in the MicroSolv PQ Excel template - FAQ
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Which size guard column should I use with my 1mm Cogent 2.o column - FAQ
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Which vial type should I use for a low volume sample study of proteins - FAQ
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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Why am I seeing multiple peaks in HPLC runs of fructose - Tips & Suggestions
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Why am I seeing two peaks for quaternium15 Dowicil-75 in LCMS - FAQ
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Why are pipettors not recommended for dispensing non aqueous liquids accurately and precisely - FAQ
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Why are the Cogent Silica-C columns more stable than other silica columns - FAQ
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Why are the same size autosampler vials listed as 2mL 1.8mL or 1.5mL by different suppliers - FAQ
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Why do I have higher pressure at a lower flow rate in my HPLC runs - FAQ
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Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column - FAQ
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Why does MicroSolv have 5 tiers or brands of autosampler vials - FAQ
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Why is isopropanol rarely used as a solvent in HPLC or LCMS methods - FAQ
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Why would I want to use an HPLC column that is not end capped - FAQ
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Wikipedia reference for use of pipets with organic solvents - Primer
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Will a different guard column hardware system different from the Cogent system affect my results when using a Cogent column - FAQ
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Will acid washing autosampler vials have consequences or affect my chromatography - FAQ
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Will different guard columns packed by different column packers be equivalent - INTERNAL ONLY
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Will I protect HPLC columns from samples that get permanently adsorbed to the column by using a syringe filter - FAQ
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Will phosphates precipitate in an HPLC column - FAQ
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Will TFA permanently alter retention / selectivity of the Cogent TYPE-C silica columns - FAQ
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Will using the Cogent Column Filters change my current HPLC results - FAQ
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Will your qualification kits for UV-vis be appropriate for my spectrophotometer - FAQ
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