HPLC Peak, Baseline & Retention Troubleshooting
/
/
/
Find practical solutions for HPLC and LC-MS troubleshooting challenges, including peak shape issues, baseline instability, retention changes, pressure fluctuations, sensitivity loss, sample preparation effects, and system-related performance problems. Learn proven approaches to diagnose and correct chromatographic issues for more reliable analytical results.
Analyzing metformin in plasma samples how do I obtain reproducible results and peak shapes - FAQ
Read More
Read More
Avoid Distorted Peak Shapes When Using a Basic Mobile Phase pH for the Analysis of a Lipophilic Amine - Tips & Suggestions
Read More
Read More
Broad Peaks That are Non Symmetrical in LCMS in Reversed Phase Solutions - Troubleshooting
Read More
Read More
Citric acid retention and peak shape has changed compared to data from a previous run what happened - FAQ
Read More
Read More
Constant Pressure Method on two Instruments Producing Different Flow Rates- Troubleshooting
Read More
Read More
Difficulties with wavelength accuracy readings while performing a PQ of an HPLC - Tips & Suggestions
Read More
Read More
Effects of Mobile Phase Ionic Strength in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
Read More
Read More
Effects of Washing and Re-using Autosampler Vials on Sample Loss and Contamination - Tech Information
Read More
Read More
Eliminating Retention Time Drift in LC-MS HILIC Methods Using Diamond Hydride Columns - Tech Information
Read More
Read More
For Sugars Analyzed by LCMS Sodium Acetate is recommended for Sensitivity of Na Adducts - Tips & Suggestions
Read More
Read More
Formic Acid Improves Peak Shapes Retention and Carryover for Polar Compounds with the Cogent Diamond Hydride HPLC Column - Tips & Suggestions
Read More
Read More
Ghost peaks appearing in results using a gradient with TFA which can degrade with time - Tips & Suggestions
Read More
Read More
Gradient Method Adjustments When Changing HPLC Column Internal Diameter - Tech Information
Read More
Read More
High Pressure Develops after Flushing a Cogent Diamond Hydride HPLC Column with Acetonitrile Cause and Solution - Tech Information
Read More
Read More
High‑Molecular‑Weight Compounds Fail to Retain on the Cogent Diamond Hydride Column - Tech Information
Read More
Read More
How Tight Should an Autosampler Vial Cap Be to Prevent Septa Push-Through During Injection - Tech Information
Read More
Read More
I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
Read More
Read More
Improving Peak Shape and Retention for Organic Acids in HILIC Methods Using Cogent Diamond Hydride Columns - Tech Information
Read More
Read More
Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
Read More
Read More
Lower Retention When Functional Group in a Compound Should be Ionized - Troubleshooting Tips
Read More
Read More
No Peaks Observed for Succinic Acid or Alpha-Ketoglutarate in LC-MS Using a Cogent Diamond Hydride Column - Tech Information
Read More
Read More
Polar and Mixed‑Polarity Metabolites Strategy Using Panel Method Cogent Diamond Hydride - Tips and Suggestions
Read More
Read More
Restoring the Initial Method Results Analyzing AMP ADP ATP UDP and GPT in Biological Extracts - Troubleshooting
Read More
Read More
Retention And or Peak Shape for Citric Acid has Changed Compared to Data from a Previous HPLC Run - Tips & Suggestions
Read More
Read More
Retention Behavior of Triphenylamine in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
Read More
Read More
Retention Time Variations Between HPLC Systems and Qualification Testing - Tech Information
Read More
Read More
Selectivity Modification in an HILIC Method Using Different Mobile Phase pH - Tips & Suggestions
Read More
Read More
Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ
Read More
Read More
Sodium Ions and Sodium Succinate Analysis in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
Read More
Read More
Storing, Conditioning, and Maintaining Cogent Diamond Hydride HPLC Columns - Tech Information
Read More
Read More
System Pressure Considerations When Using 90% Methanol and 10% Water with Cogent TYPE-C HPLC Columns - Tech Information
Read More
Read More
Troubleshooting Diminished Peak Height in Biological Extract Analysis Using Cogent Diamond Hydride Columns - Tech Information
Read More
Read More
Understanding Retention Decreases as Water Content Increases on Cogent Bidentate C18 Columns - Tech Information
Read More
Read More
Understanding the Impact of Extra Column Volume on HPLC Method Performance - Tech Information
Read More
Read More
Using a Dedicated Reversed Phase HPLC System for HILIC Methods Potential Problems and How to Avoid Them - Tech Information
Read More
Read More
What are some common sources of variance during sample preparation in an analytical application - FAQ
Read More
Read More
When and Why to Use PTFE Teflon Mobile Phase Reservoir Bottles for HPLC and LC-MS - Tech Information
Read More
Read More
When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative - Primer
Read More
Read More