Category: Trouble Shooting Suggestions
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A white film developed on my MS source so is this column packing material - FAQ
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Add 10 micro molar EDTA or medronic acid to mobile phase for better peak shapes - Tips & Suggestions
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Adjusted retention time hold up time - Primer
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Adsorption can happen quickly in an autosampler vial - Tips & Suggestions
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Advantages of using a 3.0mm ID HPLC column for LCMS - Tech Information
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Analyte retention decreases with mobile phase water content using the Cogent Bidentate C18 HPLC column has anything happened to the column - Tech Information
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Analyze phosphorylated compounds with the Cogent Diamond Hydride column - Tips & Suggestions
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Analyzing metformin in plasma samples how do I obtain reproducible results and peak shapes - FAQ
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AQ brand polypropylene MS vials compared to market leading plastic vials for LCMS - FAQ
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Aqueous Normal Phase ANP ballistic gradients compared to same in HILIC - Tips & Suggestions
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Aqueous Normal Phase ANP defined & compared to Reversed Phase & Normal Phase definitions HPLC
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Aqueous Normal Phase ANP solvent strength order - FAQ
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Are RSA-Pro X vials recommended for PFAS analysis - FAQ
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Are the AQ Advanced Quality brand of LCMS vials compatible with DMSO - FAQ
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Are the sparging stones made with a nitric acid rinse - FAQ
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Are there any solvents that I cannot use with the knitted reactor coils KRC and knitted delay tubes KDT - FAQ
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Are your HPLC and GC autosampler vials and caps sealed from outside moisture - FAQ
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Assuring retention of polar compounds in an Aqueous Normal Phase ANP gradient in positive ion mode LCMS - Tips & Suggestions
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Autoclaving glass inserts with plastic feet is not recommended - Tech Information
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Basic compound adsorption study with time dependence in vials - AppNote
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Better separation of two peaks for Reversed Phase RP HPLC - Tips & Suggestions
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Biological extracts Diamond Hydride columns and LCMS ion source diminished peak height problem - FAQ
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Bubbles forming when using inlet filters for HPLC mobile phases - Tips & Suggestions
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Bubbling or fizzing coming from a new Cogent TYPE-C column - Tech Information
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Buffer concentration can affect ANP retention of acids in HPLC Analyses - Tips & Suggestions
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Buffer is not eluting out of the Zero Flow capillary is the capillary defective - FAQ
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Can Aqueous Normal Phase ANP methods be used to separate steroids - FAQ
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Can I change the filter element in the Cogent Column Filters - FAQ
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Can I choose HPLC mobile phase percentages other than those used in the HSQ Spreadsheet - FAQ
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Can I expect a more predictable retention time with Silica-C columns than with ordinary silica - FAQ
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Can I use ion pairing reagents with the Cogent Diamond Hydride HPLC column - FAQ
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Can I use the Cogent Diamond Hydride column without mobile phase additives like formic acid - FAQ
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Can I use triethylamine TEA with Cogent Diamond Hydride columns - FAQ
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Can low quantitative results in HPLC be due to autosampler vial caps - FAQ
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Can rapid changes in column back pressure damage an HPLC column - FAQ
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Can silicone rubber be used as a cap septa at high temperature - FAQ
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Can the all silicone cap mats be sterilized - FAQ
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Can the Cogent Column Filters replace my HPLC guard columns - FAQ
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Can the HSQ Kit be used to measure the LOQ and LOD differences between instruments - FAQ
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Can the HSQ Kit be used to qualify quaternary HPLC pumps - FAQ
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Can the HSQ or PQ qualification kits be used with Waters equipment and Empower chromatography data software - FAQ
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Can you use a sparging stone for removal of dissolved oxygen - FAQ
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Causes of an HPLC column appearing to lack sensitivity in LCMS - Tips & Suggestions
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Change the type of methods used from DAD to VWD in the HSQ software - How To
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Changing analyte recovery when using syringe filters causes and suggestions - FAQ
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Changing column ID means changing your gradient - Tips & Suggestions
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Changing retention times of amino acids in biological extracts using ammonium acetate in HPLC methods. - Troubleshooting.
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Check for high pressure traced to the HPLC pump module - Tips & Suggestions
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Choose an appropriate re-equilibration or post time for gradient HPLC - How To
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Citric acid retention and peak shape has changed compared to data from a previous run what happened - FAQ
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Cleaning Cogent TYPE-C silica based HPLC columns - Tips & Suggestions
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Column cleaning and care in UHPLC columns - Tips & Suggestions
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Common cause of excessive back pressure in HPLC columns - FAQ
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Determine dwell volume of gradient HPLC systems - How To
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Determine the asymmetry factor in HPLC at 10 percent peak height - How To
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Determining efficiency of HPLC methods 4um v 2um - FAQ
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Determining Efficiency of HPLC methods comparing 4um v 2.o HPLC columns - Tips & Suggestions
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Determining wavelength accuracy below 230nm for HPLC instruments - How To
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Developing a Bromhexine HPLC method - Tips & Suggestions
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Difficulties with wavelength accuracy readings while performing a PQ of an HPLC - Tips & Suggestions
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Do all PEEK HPLC fittings fit all receiving ports - FAQ
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Do autosampler vials and caps have a temperature range - FAQ
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Do different retention times for peaks in the Resolution Test Mixture on different systems impact the integrity of my data - FAQ
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Do nylon syringe filters have a neutral membrane or do they carry a charge - FAQ
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Do Tanaka Plots for HPLC apply to columns used with Aqueous Normal Phase ANP methods - FAQ
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Does deactivation process of RSA-Pro X autosampler vials add any chlorides to the glass surface - FAQ
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Does HSQ/PQ software need to be saved as a macro enabled XLSM file - FAQ
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Dwell volume effect on HPLC analyte retention in gradient methods - Primer
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Effect of diluent on analyte adsorption to glass autosampler vials - White Paper
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Effects of washing and re-using autosampler vials on sample loss - Tech Information
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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Eliminate high background noise using a new Cogent HPLC column - Tips & Suggestions
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Eliminating shoulder peaks in an HPLC method using Cogent RP C18 columns - Tips & Suggestions
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Factors that affect sensitivity in HPLC - Tech Information
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Ferrule selection suggestions - Tech Information
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Filtering proteins which membrane to use - FAQ
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Fluctuations in readings when using the UV-Vis Calibration Kit - Tech Information
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For sugars analyzed by LCMS sodium acetate is recommended for sensitivity of Na adducts - Tips & Suggestions
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Fosetyl Aluminum analysis by HPLC - Tips & Suggestions
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Fused Insert vials that break during injection - Tips & Suggestions
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Ghost peaks appearing in results using a gradient with TFA which can degrade with time - Tips & Suggestions
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Glucose pyruvate taurocholic acid gamma glutamylcysteine and other analysis - Tips & Suggestions
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High molecular weight compounds that won't retain on Cogent Diamond Hydride column try Cogent Bidentate C8 300 - Tips & Suggestions
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High pressure develops after flushing a Cogent Diamond Hydride column with acetonitrile cause and solution - FAQ
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How a pulse from an HPLC pump affect the column and its performance - Tech Information
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How can a finger tight fitting be producing band broadening in HPLC - FAQ
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How can autosampler vials cause missed injections in HPLC - FAQ
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How can I fix split peaks with high back pressure in HPLC analyses - FAQ
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How can I make changes to locked cells in the Excel spreadsheet that comes with the HSQ qualification kit - FAQ
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How can I minimize bubble formation in limited volume vials when filling - FAQ
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How can I restore my Diamond Hydride HPLC column after I ran out of mobile phase and air was accidently introduced into it - FAQ
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How can I separate methacrylic acid and dodecyl benzene sulfonic acid - FAQ
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How can sample concentration have an effect on peak shape in HPLC - FAQ
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How can skived vs cast headspace septa affect quantitation - FAQ
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How do I keep my solvents degassed on their way to the HPLC pump - FAQ
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How do I know if a crimped cap on a vial has a proper seal - FAQ
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How do kinetics relate to the recovery in an SPE procedure - FAQ
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How do RSA autosampler vials compare against silanized vials for pharma applications - FAQ
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How does reverse EOF differ from normal EOF in Capillary Electrophoresis - FAQ
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How much higher is the pressure generated on the Cogent 2.o columns than 4um phases - FAQ
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How often do I need to clean or wash a Cogent TYPE-C column to keep data precise - FAQ
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How resistant are the ultra fine point markers to smearing - FAQ
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How similar fittings for chromatography can differ from different brands in HPLC - Tech Information
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How tight should an autosampler vial cap be to prevent push through of the septa during injection - FAQ
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How to back flush and wash an HPLC column for reuse - Tips & Suggestions
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How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
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How to eliminate retention time drift in LCMS with Aqueous Normal Phase ANP methods - Tips & Suggestions
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How to fix a saw tooth pattern in the UV baseline - Tips & Suggestions
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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How to improve LOD or detection limits in HPLC - Tips & Suggestions
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How to improve peak shapes, retention & carryover for polar compounds with the Cogent Diamond Hydride™ column - Tips & Suggestions
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How to increase HPLC UV detector response - Tips & Suggestions
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How to keep the Cogent Diamond Hydride column clean when using blood as a sample - Tips & Suggestions
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How to minimize HPLC column blockage from most common causes - Tips & Suggestions
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HPLC Retention time shift troubleshooting - Tips & Suggestions
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HPLC stationary phase can be responsible for a blocked frit- Tips & Suggestions
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I am not observing any peaks for succinic acid or alpha-ketoglutarate with the Cogent Diamond Hydride column - Tips & Suggestions
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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
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I see black spots in my amber vials are they be a source of contamination - FAQ
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I see extra peaks in my chromatography when I am not using the same filter - Tips & Suggestions
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If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
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If I am using TFA in HPLC will I have a problem with my Delrin type fittings - FAQ
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If I do not remove gas from my mobile phase solvents what are the consequences - FAQ
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If I use a buffer at pH 9 at 30 to 35C with the Zero Flow capillaries what lifetime should I expect from them - FAQ
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If you are not seeing an expected peak in an HPLC assay - Tips & Suggestions
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Importance of Fresh Cutting Edges in Tubing– Tech Information
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Improve or obtain flat baselines in HPLC methods with a gradient - Tips & Suggestions
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Improving peak shapes in an HPLC method in aqueous normal phase ANP - Tips & Suggestions
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Inconsistent peak heights obtained for glucosamine in HPLC - Tips & Suggetions
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Increasing selectivity and resolution for a xylose and ribose method - Tips & Suggestions
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Injection volume suggestions for Cogent TYPE-C HPLC columns - Tips & Suggestions
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Integrate a peak with a shoulder - How to
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Is formic acid compatible with borosilicate glass - FAQ
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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
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Is there any Bismuth in Soft-Guard™ Septa - FAQ
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Issues arising from changes in mobile phase with acids to one with ammonium salts. Tips & Suggestions
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Issues with a steep UV trace slope in gradient HPLC methods - Tips & Suggestions
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Know when to discard an outlier data point in HPLC by using the Q Test - How To
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Lower retention is observed when functional group in a compound is ionized - Tips & Suggestions
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Maximum Pressure chart for 1/8" and 1/4" stainless steel tubing - Tech Information
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Maximum pressure chart for stainless steel tubing - Tech Information
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Maximum recommended flow rates for the different filter pore sizes. – Tech Information
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Method development tip for maleic hydrazide - Tips & Suggestions
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Missing peaks using UV detection in HPLC methods - Tech Information
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Modify selectivity in an ANP method of HPLC using different mobile phase pH - Tips & Suggestions
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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Negative Baselines in HPLC at 214nm - Tips & Suggestions
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No need to use a high pH mobile phases for compounds with a high pKa - Tips & Suggestions
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Obtain a flat baseline in a gradient method without using blank subtraction - How to
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Octanol Water Partition coefficient - Primer
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Peak area decreases with increasing flow rate - FAQ
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Peaks are broad and non symmetrical in LCMS method using Reversed Phase columns - Tips & Suggestions
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Percentage of acid needed in Aqueous Normal Phase ANP methods - Tips & Suggestions
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PFAS and other contaminants leaching into an analysis - Tips & Suggestions
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Policy on verifying a new Cogent HPLC column performance - Tips & Suggestions
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Poor HPLC peak shape can be due to injection sample mismatch - Tips & Suggestions
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Poor peak shape for ethylenediamine in LCMS analyses - Tech Information
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Protecting HPLC analytical columns and when to use guard columns vs Cogent Column Filters - Tips & Suggestions
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Purge metals from HPLC system using EDTA or medronic acid - How To
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Quaternary amines analysis by LCM and finding the right ion - How To
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Random shifting of retention times in HPLC chromatograms - Tips & Suggestions
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Relative response factor RRF and correction factor - How To
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Remove a ferrule that is stuck in an HPLC system or column- How To
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Restoring the initial results with a method for analyzing AMP ADP ATP UDP and GPT in biological extracts - Tips & Suggestions
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Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run - Tips & Suggestions
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Retention time varies from instrument to instrument - Tips & Suggestions
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Ribose and Xylose analysis without forming Schiff bases - AppNote
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RSA-Pro X vial improve quantitation precision with HPLC – AppNote
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Sample concentration changes can effect retention time in HPLC - Primer
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Screw and snap cap evaporation studies - Tech Information
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Sensitivity slowly diminishes in HPLC - Tips & Suggestions
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Should I filter my HPLC solvents and buffers before use - FAQ
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Silanizing glass autosampler vial or inserts for surface deactivation - Tech Information
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ
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Solvents and solutions that should be avoided when using PEEK filters fittings or tubing - Tech Information
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Solving baseline problems in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Store and maintain Cogent Diamond Hydride HPLC columns - How To
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Suggestion for separation of phenylephrine HCl acetaminophen and dextromethorphan - Tips & Suggestions
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Techniques to prepare a 0.05% solution from a tablet for HPLC injection - How to
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The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase - Tips & Suggestions
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Tip for working with plasma samples and Cogent TYPE-C HPLC columns - Tips & Suggestions
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To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
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Troubleshooting problems with poor HPLC runs before the examining the column - Tips & Suggestions
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Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run - Tips & Suggestions
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Use a linear flow rate test to check back pressure on an HPLC column - Tech Information
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Use Teflon bottles for A and B solvents in negative ion mode LCMS - Primer
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Using a Constant Pressure Method on two Instruments Producing Different Flow Rates- Tips & Suggestions
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Using ammonium acetate or triethylamine TEA with Cogent HPLC columns - Tips & Suggestions
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Using an Aqueous Normal Phase ANP method will sodium ions interfere with succinate when analyzing sodium succinate - FAQ
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Using inorganic buffers for HPLC UV analysis of citric acid - Tips & Suggestions
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Using methanol instead of acetonitrile in an HPLC method should be done with caution - Tips & Suggestions
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Using the wavelength calibration solution holmium oxide for HPLC UV detector performance qualification - Tips & Suggestions
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Voltage effects in Capillary Electrophoresis - Tech Information
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What are some common sources of variance during sample preparation in an analytical application - FAQ
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What are the pH ranges of common buffers for HPLC - Tips & Suggestions
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What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
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What do You suggest for an internal standard in glucosamine LCMS Analysis - FAQ
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What does Post Time mean in an LCMS or HPLC gradient method - FAQ
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What effect does high injection volume have on column performance in Aqueous Normal Phase ANP HPLC- FAQ
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What is a system peak as defined for HPLC - Primer
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What is considered an extractable that comes from syringe filters - FAQ
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What is peak inhibition in LCMS - FAQ
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What is pKa and how is it related to pH in HPLC mobile phases and sample diluents - FAQ
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What is Post Time in an HPLC gradient method - Primer
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What is the advantage of using a Pharmafix type of septa for headspace analysis - FAQ
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What is the cause of a pressure increase after injections of blanks in HPLC - FAQ
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What is the difference between a Waters type fitting and a standard 10-32 fitting - FAQ
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What is the effect of extra column volume for different HPLC instruments on the same method - FAQ
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What is the maximum concentration of ammonium fluoride to use in a mobile phase to avoid column damage - Tips & Suggestions
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What is the maximum salt concentration to use with Cogent TYPE-C HPLC columns with HPLC or LCMS - FAQ
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What is the maximum temperature for Cogent Diamond Hydride HPLC columns - FAQ
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What is the most effective way to clean HPLC columns after or before use - Tips & Suggestions
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What is the purpose of a partial slit septa - FAQ
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What is the purpose of a pre slit septa - FAQ
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What is the purpose of back flushing an HPLC column and will it damage it - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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What is the recommended pH range for Cogent Diamond Hydride HPLC columns - FAQ
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What is the useful temperature range of 316 stainless steel laboratory products - FAQ
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What is the useful temperature range of PEEK laboratory products - FAQ
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What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time - FAQ
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What results when I use a small guard column with a larger ID or prep column - FAQ
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What type of tubing do I use to connect the guard column holder to analytical columns - FAQ
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What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column - FAQ
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When and why use Teflon bottles for your mobile phase reservoirs - FAQ
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When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative - Primer
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When is the acid group of the Cogent UDA HPLC column ionized - Tech Information
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When to use snap caps vs screw caps preventing evaporation - FAQ
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Where a static mixer for HPLC instruments should be placed - Tips & Suggestions
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Which columns are compatible with the Hichrom brand column coupler Cat No 47450-06 - FAQ
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Which ferrules are best when using soft polymeric tubing - FAQ
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Which HPLC column do you suggest for analysis of carotene or lycopene - FAQ
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Which membrane is best for aqueous buffers when using syringe filters - FAQ
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Which solvents should I avoid with AQ syringe filters that have a nylon membrane - FAQ
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Which solvents should not be used with the Cogent Column Filters - FAQ
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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Why am I seeing multiple peaks in HPLC runs of fructose - Tips & Suggestions
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Why am I seeing two peaks for quaternium15 Dowicil-75 in LCMS - FAQ
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Why am I seeing two peaks for quaternium-15 or Dowicil-75 in my LCMS data - FAQ
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Why are environmental conditions such as temperature relative humidity that are not specified on the CofA for the UV Calibration Kit - FAQ
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Why are pipettors not recommended for dispensing non aqueous liquids accurately and precisely - FAQ
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Why do I have higher pressure at a lower flow rate in my HPLC runs - FAQ
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Why do I have lower than normal back pressure with Cogent Diamond Hydride HPLC columns - FAQ
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Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column - FAQ
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Why does the color of the Holmium Oxide solution for HPLC performance qualification look different than before - FAQ
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Why doesn't triphenylamine a tertiary amine retain in Aqueous Normal Phase ANP - FAQ
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Why is filtering of water samples very slow when using PTFE membrane filters - FAQ
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Why is isopropanol rarely used as a solvent in HPLC or LCMS methods - FAQ
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Why is too much background electrolyte BGE a bad thing in CE - FAQ
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Why you should use temperature control with the UDC-Cholesterol column - Tips & Suggestions
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Will a different guard column hardware system different from the Cogent system affect my results when using a Cogent column - FAQ
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Will acid washing autosampler vials have consequences or affect my chromatography - FAQ
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Will different guard columns packed by different column packers be equivalent - INTERNAL ONLY
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Will I protect HPLC columns from samples that get permanently adsorbed to the column by using a syringe filter - FAQ
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Will I see any differences in my HPLC chromatography if I change to AQ syringe filters - FAQ
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Will phosphates precipitate in an HPLC column - FAQ
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Will using the Cogent Column Filters change my current HPLC results - FAQ
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