Category: HPLC Tips and Suggestions
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A white film developed on my MS source so is this column packing material - FAQ
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Acetone as the organic mobile phase component - AppNote
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Achieve optimal seals when crimping caps on vials - How to
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Add 10 micro molar EDTA or medronic acid to mobile phase for better peak shapes - Tips & Suggestions
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Adjusted retention time hold up time - Primer
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Adjusted retention time in HPLC - HPLC Primer
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Adsorption can happen quickly in an autosampler vial - Tips & Suggestions
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Advantages of using a 3.0mm ID HPLC column for LCMS - Tech Information
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Amide or amino HPLC columns. - Tech Information
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Analysis of Litchi seed extracts - AppNote
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Analyte retention decreases with mobile phase water content using the Cogent Bidentate C18 HPLC column has anything happened to the column - Tech Information
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Analyze phosphorylated compounds with the Cogent Diamond Hydride column - Tips & Suggestions
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Analyzing metformin in plasma samples how do I obtain reproducible results and peak shapes - FAQ
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Aqueous Normal Phase ANP and how can it is useful for polar compounds - Tech Information
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Aqueous Normal Phase ANP ballistic gradients compared to same in HILIC - Tips & Suggestions
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Aqueous Normal Phase ANP defined & compared to Reversed Phase & Normal Phase definitions HPLC
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Aqueous Normal Phase ANP HPLC retention mechanism elucidated - Tech Information
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Aqueous Normal Phase ANP HPLC what is it - FAQ
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Aqueous Normal Phase ANP solvent strength order - FAQ
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Are column oven temperatures qualified by the HSQ Kit for HPLC - FAQ
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Are RSA vials suitable for elemental analysis - FAQ
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Are RSA-Pro X vials recommended for PFAS analysis - FAQ
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Are sparging stones good for ozonating water - FAQ
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Are the AQ Advanced Quality brand of LCMS vials compatible with DMSO - FAQ
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Are there HPLC columns that could be used to separate both polar and nonpolar compounds in a single run - FAQ
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Assuring retention of polar compounds in an Aqueous Normal Phase ANP gradient in positive ion mode LCMS - Tips & Suggestions
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Autoclaving glass inserts with plastic feet is not recommended - Tech Information
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Avoid loss of concentration of heparin with HPLC when using syringe filters - Tips & Suggestions
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Avoid microbial growth in HPLC mobile phases - How to
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Basic compound adsorption study with time dependence in vials - AppNote
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Better methods than HILIC in HPLC and LCMS - Tips & Suggestions
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Better separation of two peaks for Reversed Phase RP HPLC - Tips & Suggestions
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Biological extracts Diamond Hydride columns and LCMS ion source diminished peak height problem - FAQ
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Blocked HPLC column from proteins from the sample matrix can be recovered - Tips & Suggestions
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Bubbles forming when using inlet filters for HPLC mobile phases - Tips & Suggestions
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Bubbling or fizzing coming from a new Cogent TYPE-C column - Tech Information
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Buffer concentration can affect ANP retention of acids in HPLC Analyses - Tips & Suggestions
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Calculate assay of lisinopril dihydrate based on the anhydrous form - How to
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Calculate the internal volume of tubing - How to
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Calculate the ligand density of a bonded phase of an HPLC column - How To
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Calculating theoretical plates N at half height - How To
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Can amber or clear polypropylene vials be sterilized with autoclaving or ethylene oxide - FAQ
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Can Aqueous Normal Phase ANP methods be used to separate steroids - FAQ
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Can Cogent TYPE-C HPLC columns be used in supercritical fluid chromatography SFC - FAQ
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Can I backflush the Cogent column filter for longer in-service life - FAQ
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Can I choose HPLC mobile phase percentages other than those used in the HSQ Spreadsheet - FAQ
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Can I make a PTFE syringe filter more hydrophilic - FAQ
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Can I use a silica or Silica-C guard column with an analytical column of a different phase - FAQ
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Can I use Acetone as a mobile phase solvent in Aqueous Normal Phase ANP - FAQ
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Can I use ion pairing reagents with the Cogent Diamond Hydride HPLC column - FAQ
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Can I use of the Cogent Diamond Hydride HPLC column above pH 7 in HPLC - FAQ
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Can I use phosphoric acid or sodium phosphate with the Cogent Diamond Hydride HPLC column - FAQ
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Can I use qualification solutions with different lot numbers - FAQ
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Can I use the certified HPLC column supplied in the HSQ Kit for UHPLC performance qualification - FAQ
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Can I use the Cogent Diamond Hydride column without mobile phase additives like formic acid - FAQ
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Can I use the holmium oxide solution in a UV instrument that calls for Starna or NIST cuvettes - FAQ
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Can I use the knitted reactor coils KRC with my HPLC fluorescence detector - FAQ
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Can I use the tubing plier cutters with 1/16 or 1/8 OD stainless steel HPLC tubing - FAQ
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Can I use the UV-vis Calibration Kit to qualify detection at 200nm - FAQ
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Can I use triethylamine TEA with a Cogent Silica-C column - FAQ
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Can I use triethylamine TEA with Cogent Diamond Hydride columns - FAQ
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Can I use trifluoroacetic acid TFA to improve the chromatographic performance in LCMS - FAQ
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Can low quantitative results in HPLC be due to autosampler vial caps - FAQ
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Can rapid changes in column back pressure damage an HPLC column - FAQ
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Can Reversed Phase and ANP mechanisms operate with the same effect at the same time - FAQ
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Can the BASIK brand filter degasser be used to de-gas carbon dioxide from a solvent - FAQ
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Can the Cogent Column Filters replace my HPLC guard columns - FAQ
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Can the HSQ Kit be used to measure the LOQ and LOD differences between instruments - FAQ
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Can the HSQ or PQ qualification kits be used with Waters equipment and Empower chromatography data software - FAQ
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Can the L3 Linearity Solution in the HSQ kit be used for UV wavelength accuracy - FAQ
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Can the Ultra-T brand of PEEK tubing be used in medical applications - FAQ
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Can you measure EDTA plasma samples for creatine & creatinine - FAQ
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Causes of an HPLC column appearing to lack sensitivity in LCMS - Tips & Suggestions
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Causes of high baseline noise with Cogent Diamond Hydride HPLC column - Tips & Suggestions
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Changing analyte recovery when using syringe filters causes and suggestions - FAQ
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Changing column ID means changing your gradient - Tips & Suggestions
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Check for high pressure traced to the HPLC pump module - Tips & Suggestions
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Choose an appropriate guard column when using a Cogent TYPE-C HPLC column - How To
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Choose an appropriate re-equilibration or post time for gradient HPLC - How To
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Choosing the correct HPLC column coupler - How To
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Citric acid retention and peak shape has changed compared to data from a previous run what happened - FAQ
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Cleaning Cogent TYPE-C silica based HPLC columns - Tips & Suggestions
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Cogent amino HPLC columns designations for reversed or normal phase - Tech Information
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Cogent silica hydride HPLC columns by order of hydrophobicity - Tips & Suggestions
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Cogent TYPE-C columns used in prep chromatography offer value - Tips & Suggestions
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Column cleaning and care in UHPLC columns - Tips & Suggestions
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Common cause of excessive back pressure in HPLC columns - FAQ
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Convert HPLC columns between Reversed Phase and Normal Phase - How to
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Converting Cogent TYPE-C columns to normal phase after use in reversed phase - How To
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Cut PEEK tubing using a Clean Cut90 cutter - How to
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Cut stainless steel tubing for HPLC properly - How to
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Cysteine analysis tips for LCMS - Tips & Suggestions
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Determine dwell volume of gradient HPLC systems - How To
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Determine limits of detection LOD and limits of quantification LOQ - How To
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Determine pore volume of an HPLC column using HPLC injections - How To
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Determine the asymmetry factor in HPLC at 10 percent peak height - How To
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Determining efficiency of HPLC methods 4um v 2um - FAQ
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Determining Efficiency of HPLC methods comparing 4um v 2.o HPLC columns - Tips & Suggestions
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Determining HPLC method accuracy - How To
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Determining the precision of an HPLC method - Tips & Suggestions
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Developing a Bromhexine HPLC method - Tips & Suggestions
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Difference between adsorption and absorption - FAQ
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Difference between assay and purity regarding pharmaceutical analysis - Primer
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Difficulties with wavelength accuracy readings while performing a PQ of an HPLC - Tips & Suggestions
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Do nylon syringe filters have a neutral membrane or do they carry a charge - FAQ
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Do the Cogent Silica-C columns contain any carbon - FAQ
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Does the method used in the HSQ Kit for HPLC need to be validated - FAQ
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Dwell volume effect on HPLC analyte retention in gradient methods - Primer
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Effects of washing and re-using autosampler vials on sample loss - Tech Information
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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Eliminate high background noise using a new Cogent HPLC column - Tips & Suggestions
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Eliminating shoulder peaks in an HPLC method using Cogent RP C18 columns - Tips & Suggestions
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Establishing HPLC method linearity - Tips & Suggestions
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Factors that affect sensitivity in HPLC - Tech Information
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Fluctuations in readings when using the UV-Vis Calibration Kit - Tech Information
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For Normal Phase HPLC which syringe filter do you recommend - FAQ
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For sugars analyzed by LCMS sodium acetate is recommended for sensitivity of Na adducts - Tips & Suggestions
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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Fosetyl Aluminum analysis by HPLC - Tips & Suggestions
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Fosetyl aluminum analysis by Reversed Phase HPLC - Tips & Suggestions
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Fused Insert vials that break during injection - Tips & Suggestions
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Ghost peaks appearing in results using a gradient with TFA which can degrade with time - Tips & Suggestions
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Glucose pyruvate taurocholic acid gamma glutamylcysteine and other analysis - Tips & Suggestions
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Guidelines for packing in the PEEK column hardware - Tips & Suggestions
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Has there been a risk assessment for the use of SOLVFil in GMP laboratories - FAQ
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Helpful HPLC columns you may not know about - White Paper
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High molecular weight compounds that won't retain on Cogent Diamond Hydride column try Cogent Bidentate C8 300 - Tips & Suggestions
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High pressure develops after flushing a Cogent Diamond Hydride column with acetonitrile cause and solution - FAQ
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How a pulse from an HPLC pump affect the column and its performance - Tech Information
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How can a finger tight fitting be producing band broadening in HPLC - FAQ
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How can autosampler vials cause missed injections in HPLC - FAQ
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How can Cogent TYPE-C HPLC columns offer faster results compared to other type of silica based columns - FAQ
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How can I fix poor peak shape for phosphorylated compounds in aqueous normal phase ANP methods - FAQ
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How can I fix split peaks with high back pressure in HPLC analyses - FAQ
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How can I get separation of tartaric and malic acid in a complex mixture? - FAQ.
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How can I improve accuracy and precision when separating polar compounds by HPLC using Aqueous Normal Phase ANP - FAQ
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How can I minimize bubble formation in limited volume vials when filling - FAQ
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How can I separate capsaicinoids in hot sauce by HPLC - Tips & Suggestions
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How can I separate methacrylic acid and dodecyl benzene sulfonic acid - FAQ
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How can sample concentration have an effect on peak shape in HPLC - FAQ
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How do I keep my solvents degassed on their way to the HPLC pump - FAQ
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How does ammonium fluoride affect chromatography using Cogent TYPE-C HPLC columns?
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How does ammonium fluoride affect HPLC and LCMS data if using Aqueous Normal Phase ANP methods - FAQ
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How does the Cogent UDA HPLC column's ligand bond to the silica hydride surface - FAQ
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How long will it take for chloroform to degrade the plastic safety coating on the mobile phase bottles if the bottle is broken during a fall - FAQ
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How much higher is the pressure generated on the Cogent 2.o columns than 4um phases - FAQ
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How often do I need to clean or wash a Cogent TYPE-C column to keep data precise - FAQ
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How often do I need to do the conditioning procedure for a new Diamond Hydride column - FAQ
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How resistant are the ultra fine point markers to smearing - FAQ
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How similar fittings for chromatography can differ from different brands in HPLC - Tech Information
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How tight should an autosampler vial cap be to prevent push through of the septa during injection - FAQ
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How to back flush and wash an HPLC column for reuse - Tips & Suggestions
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How to calibrate UV-vis and HPLC detectors with NIST traceable solutions below 230nm - Tips & Suggestions
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How to change ferrules on Di-Ad HPLC column connectors - Tips & Suggestions
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How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
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How to clean sparging stones and In line SS filters - Tips & Suggestions
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How to condition Cogent RP or HPS or Axis HPLC columns for storage - Tips & Suggestions
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How to define custom plate measurements for the U-2D Micro-Sample Management System in the Waters Acquity console software - How to
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How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
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How to eliminate retention time drift in LCMS with Aqueous Normal Phase ANP methods - Tips & Suggestions
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How to fix a saw tooth pattern in the UV baseline - Tips & Suggestions
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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How to handle sparging stones to avoid contamination of mobile phases - Tips & Suggestions
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How to improve LOD or detection limits in HPLC - Tips & Suggestions
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How to improve peak shapes, retention & carryover for polar compounds with the Cogent Diamond Hydride™ column - Tips & Suggestions
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How to increase HPLC UV detector response - Tips & Suggestions
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How to keep HPLC columns free of adsorbed contaminants - How To
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How to keep surface treated frits from biofouling - FAQ
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How to keep the Cogent Diamond Hydride column clean when using blood as a sample - Tips & Suggestions
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How to minimize HPLC column blockage from most common causes - Tips & Suggestions
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How to prepare 10mM ammonium acetate buffer 90:10 in acetonitrile for HPLC and LCMS - Tips & Suggestions
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How to prepare 16mM ammonium acetate buffer 90:10 in acetonitrile for HPLC - Tips & Suggestions
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How to separate phospholipids with HPLC - Tips & Suggestions
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How to tighten screw caps properly - Tips & Suggestions
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HPLC Method Linearity tip.
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HPLC Retention time shift troubleshooting - Tips & Suggestions
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HPLC stationary phase can be responsible for a blocked frit- Tips & Suggestions
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I am not observing any peaks for succinic acid or alpha-ketoglutarate with the Cogent Diamond Hydride column - Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
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I see extra peaks in my chromatography when I am not using the same filter - Tips & Suggestions
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Identify used U-2D inserts for multiple projects or in small sample sets - Tips & Suggestions
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If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
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If I do not remove gas from my mobile phase solvents what are the consequences - FAQ
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If you are not seeing an expected peak in an HPLC assay - Tips & Suggestions
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Importance of Fresh Cutting Edges in Tubing– Tech Information
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Improve or obtain flat baselines in HPLC methods with a gradient - Tips & Suggestions
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Improvement in signal to noise in LCMS with an ANP method - Tips & Suggestions
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Improving peak shapes in an HPLC method in aqueous normal phase ANP - Tips & Suggestions
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Inconsistent peak heights obtained for glucosamine in HPLC - Tips & Suggetions
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Increasing selectivity and resolution for a xylose and ribose method - Tips & Suggestions
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Injection Technique for Aqueous Samples using Cogent TYPE-C Columns in ANP - Tips & Suggestions
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Injection volume suggestions for Cogent TYPE-C HPLC columns - Tips & Suggestions
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Integrate a peak with a shoulder - How to
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Interpreting mass spectral data - Primer
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Is formic acid compatible with borosilicate glass - FAQ
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Is PEEK tubing or PEEK fittings autoclavable - FAQ
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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
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Is the Cogent Column Filter compatible with a 2.1mm ID column - FAQ
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Is there a difference in the properties of different colors of rubber in vial cap septa - FAQ
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Is there any Bismuth in Soft-Guard™ Septa - FAQ
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Issues arising from changes in mobile phase with acids to one with ammonium salts. Tips & Suggestions
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Issues with a steep UV trace slope in gradient HPLC methods - Tips & Suggestions
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Know the void volume of an HPLC column - Tips & Suggestions
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Know when to discard an outlier data point in HPLC by using the Q Test - How To
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LFER studies compared to Log D data in describing the Aqueous Normal Phase ANP retention - Tips & Suggestions
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Lower retention is observed when functional group in a compound is ionized - Tips & Suggestions
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Make PTFE syringe filters hydrophilic - How to
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Maximum recommended flow rates for the different filter pore sizes. – Tech Information
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Measure signal to noise ratio in an HPLC chromatogram - How to
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Measurements of U-2D Micro-Sample Management System - Tech Information
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Metal free stainless steel HPLC columns comparison to PEEK column performance - Tips & Suggestions
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Method development tip for maleic hydrazide - Tips & Suggestions
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Missing peaks using UV detection in HPLC methods - Tech Information
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Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
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Modify selectivity in an ANP method of HPLC using different mobile phase pH - Tips & Suggestions
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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My retention times are changing after storing my buffer and what could be causing this and tips to help avoid issues - FAQ
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Negative Baselines in HPLC at 214nm - Tips & Suggestions
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No need to use a high pH mobile phases for compounds with a high pKa - Tips & Suggestions
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Obtain a flat baseline in a gradient method without using blank subtraction - How to
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Octanol Water Partition coefficient - Primer
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Peak area decreases with increasing flow rate - FAQ
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Peaks are broad and non symmetrical in LCMS method using Reversed Phase columns - Tips & Suggestions
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Percentage of acid needed in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Performance goals or objectives for a routine HPLC method - Tips & Suggestions
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PFAS analysis using glass or plastic vials - Tips and Suggestions
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PFAS and other contaminants leaching into an analysis - Tips & Suggestions
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Phospholipids analyzed with LCMS - AppNote
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Poor HPLC peak shape can be due to injection sample mismatch - Tips & Suggestions
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Poor peak shape for ethylenediamine in LCMS analyses - Tech Information
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Pre mix your diluent and mobile phase in sample preparation for HPLC - Tips & Suggestions
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Precision and suitability of the Cogent TYPE-C Silica HPLC columns in LC-MS
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Purge metals from HPLC system using EDTA or medronic acid - How To
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Quaternary amines analysis by LCM and finding the right ion - How To
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Random shifting of retention times in HPLC chromatograms - Tips & Suggestions
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Reasons for using direct adaptive Di-Ad HPLC column connectors - Tips & Suggestions
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Recover an HPLC column that has had ammonium acetate precipitated in it - How To
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Relative response factor RRF and correction factor - How To
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Reliability of wet chemical assays versus HPLC assays - Primer
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Remove a ferrule that is stuck in an HPLC system or column- How To
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Rescaling graphs in the MicroSolv HPLC PQ Kit Excel template - Tips & Suggestions
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Restoring the initial results with a method for analyzing AMP ADP ATP UDP and GPT in biological extracts - Tips & Suggestions
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Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run - Tips & Suggestions
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Retention time varies from instrument to instrument - Tips & Suggestions
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Ribose and Xylose analysis without forming Schiff bases - AppNote
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RSA-Pro X vial improve quantitation precision with HPLC – AppNote
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Sample concentration changes can effect retention time in HPLC - Primer
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Sample diluents should match your starting mobile phase - Tips & Suggestions
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Screw and snap cap evaporation studies - Tech Information
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Sensitivity slowly diminishes in HPLC - Tips & Suggestions
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Should I filter my HPLC solvents and buffers before use - FAQ
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Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP - FAQ
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Silanizing glass autosampler vial or inserts for surface deactivation - Tech Information
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Silicone rubber in septa for autosampler vials vary in the way it is made - Tech Information
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Silicone rubber used in septa for autosampler vials purity variations - Tech Information
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Solving baseline problems in ANP methods with the Cogent Diamond Hydride column - Tips & Suggestions
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Solving baseline problems in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Spurious peaks from nylon syringe filters with DI water using electrochemical detection can be avoided - Tips & Suggestions
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Store and condition Cogent TYPE-C HPLC columns - How To
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Store and maintain Cogent Diamond Hydride HPLC columns - How To
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Storing the HSQ & PQ Kit test solutions when not in use - How To
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Suggestion for separation of phenylephrine HCl acetaminophen and dextromethorphan - Tips & Suggestions
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Suggestions for HPLC method development with silica hydride columns - Tips & Suggestions
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Suggestions on how to separate capsaicinoids in hot sauce by HPLC - Tips & Suggestions
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System suitability requirements for a USP HPLC method - Tips & Suggestions
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Techniques to prepare a 0.05% solution from a tablet for HPLC injection - How to
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Terminology and criteria for chirality - Primer
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Terminology and Criteria for Chirality - Primer
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The best way to avoid with ion suppression issues in LCMS if you need to use an ion-pair agent - Primer
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The common names of HPLC column diameters - Primer
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The difference between potency and purity in drug products - Tips & Suggestions
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The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase - Tips & Suggestions
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Theoretical plate estimates indicates column condition - Primer
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Theoretical plate N estimate for real samples - How to
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Tip for working with plasma samples and Cogent TYPE-C HPLC columns - Tips & Suggestions
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Tizanidine HCl HPLC Method transferred to near UHPLC - AppNote
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To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
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Troubleshooting problems with poor HPLC runs before the examining the column - Tips & Suggestions
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Two gradients to use for analysis of hydrophilic and or hydrophobic molecules in the same Aqueous Normal Phase ANP method - Tips & Suggestions
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Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run - Tips & Suggestions
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Types of isomers and suitable HPLC columns for separation - Tips & Suggestions
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Use a linear flow rate test to check back pressure on an HPLC column - Tech Information
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Use bubble point to filter water through hydrophobic membranes - How to
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Use Teflon bottles for A and B solvents in negative ion mode LCMS - Primer
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Using a Constant Pressure Method on two Instruments Producing Different Flow Rates- Tips & Suggestions
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Using ammonium acetate or ammonium formate in the Diamond Hydride - Tips & Suggestions
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Using ammonium acetate or formate in an acetonitrile based HPLC mobile phase for Aqueous Normal Phase ANP - Tips & Suggestions
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Using ammonium acetate or triethylamine TEA with Cogent HPLC columns - Tips & Suggestions
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Using an Aqueous Normal Phase ANP method will sodium ions interfere with succinate when analyzing sodium succinate - FAQ
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Using Aqueous Normal Phase ANP to your advantage - White Paper
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Using inorganic buffers for HPLC UV analysis of citric acid - Tips & Suggestions
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Using knitted delay tubes KDTs in mass directed prep chromatography - How To
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Using methanol instead of acetonitrile in an HPLC method should be done with caution - Tips & Suggestions
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Using one syringe filter for many samples will I get sample carry over - FAQ
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Using temperature changes for separations of terpenoids with the Cogent Bidentate C18 and UDC-Cholesterol HPLC columns - Tips & Suggestions
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Using the wavelength calibration solution holmium oxide for HPLC UV detector performance qualification - Tips & Suggestions
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Using triethylamine TEA in Aqueous Normal Phase ANP methods for simple sugars - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What are some common sources of variance during sample preparation in an analytical application - FAQ
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What are some of the advantages of using Cogent TYPE-C prep and semi-prep HPLC columns - FAQ
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What are the advantages and disadvantages of acid washing RSA vials - FAQ
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What are the maximum changes allowed in a USP method- FAQ
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What are the pH ranges of common buffers for HPLC - Tips & Suggestions
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What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
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What are theoretical plates and HETP in HPLC - Primer
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What are your definitions of normal phase reversed phase and ANP in HPLC - FAQ
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What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
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What can damage the surface treatment of silanized glass vials - FAQ
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What column changes are permitted in USP HPLC methods - FAQ
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What determines the retention of a molecule in reversed phase HPLC - FAQ
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What do You suggest for an internal standard in glucosamine LCMS Analysis - FAQ
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What does analytical method validation entail for HPLC and LCMS - Primer
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What does Post Time mean in an LCMS or HPLC gradient method - FAQ
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What does the term Activity refer to in chromatography - FAQ
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What effect does high injection volume have on column performance in Aqueous Normal Phase ANP HPLC- FAQ
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What inside diameter ID of standard tubing is most suitable for HPLC mobile phase - FAQ
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What is 0.1 % w/v ammonium acetate in terms of molarity - FAQ
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What is a base deactivated HPLC Column - FAQ
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What is a master batch for Cogent HPLC columns - FAQ
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What is a steady state for surfaces like syringe filters - FAQ
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What is a system peak as defined for HPLC - Primer
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What is considered an extractable that comes from syringe filters - FAQ
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What is peak inhibition in LCMS - FAQ
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What is pKa and how is it related to pH in HPLC mobile phases and sample diluents - FAQ
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What is Post Time in an HPLC gradient method - Primer
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What is TFA and when should I use it - Primer
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What is the best way to track samples in U-2D inserts - Tips and Suggestions
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What is the Bubbling or Fizzing Coming from a New Cogent TYPE-C Column? - FAQ
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What is the cause of a pressure increase after injections of blanks in HPLC - FAQ
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What is the compatibility of Cogent TYPE-C Silica HPLC columns with UHPLC instruments - FAQ
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What is the definition of passivating and purging in HPLC - FAQ
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What is the difference between a low and high pressure union in HPLC - FAQ
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What is the difference between a union and an adapter in HPLC - FAQ
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What is the difference between a Waters type fitting and a standard 10-32 fitting - FAQ
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What is the difference between AQ brand and low cost syringe filter brands - FAQ
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What is the difference between precision point and conical point autosampler vial inserts - FAQ
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What is the difference between related compounds and impurities - FAQ
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What is the difference between related compounds and related substances in pharmaceutical HPLC - FAQ
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What is the difference between the terms 45 shore A and 55 shore A in septa - FAQ
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What is the difference between weak and strong cation exchange for Ion Exchange columns - FAQ
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What is the durability of the SOLVFIL when used with standard mobile phases and buffered mobile phases - FAQ
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What is the effect of extra column volume for different HPLC instruments on the same method - FAQ
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What is the maximum concentration of ammonium fluoride to use in a mobile phase to avoid column damage - Tips & Suggestions
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What is the maximum loading capacity mass injection volume that can be used for a semi prep Cogent TYPE-C HPLC column - FAQ
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What is the maximum pressure Cogent HPLC columns can tolerate - FAQ
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What is the maximum pressure of your guard columns - FAQ
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What is the maximum salt concentration to use with Cogent TYPE-C HPLC columns with HPLC or LCMS - FAQ
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What is the most effective way to clean HPLC columns after or before use - Tips & Suggestions
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What is the pilot length or dimension X depth for end fittings uses to connect HPLC columns - FAQ
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What is the purpose of a partial slit septa - FAQ
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What is the purpose of a pre slit septa - FAQ
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What is the purpose of back flushing an HPLC column and will it damage it - FAQ
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What is the purpose of different bonded phases using Cogent TYPE-C Silica - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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What is the recommended pH range for Cogent Diamond Hydride HPLC columns - FAQ
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What is the temperature range of MicroSolv brand autosampler vial screw caps - FAQ
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What is the useful temperature range of 316 stainless steel laboratory products - FAQ
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What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time - FAQ
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What results when I use a small guard column with a larger ID or prep column - FAQ
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What special care should I take to protect my HPLC columns when I am equilibrating them - FAQ
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What type of fittings can I use in mobile phase connections - FAQ
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What type of tubing do I use to connect the guard column holder to analytical columns - FAQ
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What type or brand of injector should I use with the 2 µL sample loop - FAQ
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What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column - FAQ
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What would you recommend to reduce time to develop an HPLC method - Tips & Suggestions
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When and why use Teflon bottles for your mobile phase reservoirs - FAQ
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When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative - Primer
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When is the acid group of the Cogent UDA HPLC column ionized - Tech Information
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When to use PEEK or stainless steel for HPLC column hardware - Tips & Suggestions
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Where a static mixer for HPLC instruments should be placed - Tips & Suggestions
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Which acid or base additive should I choose for positive or negative ion mode in LC-MS - FAQ
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Which column do you suggest for Cocaine & metabolite Ecognin separation - Tips & Suggestions
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Which columns are compatible with the Hichrom brand column coupler Cat No 47450-06 - FAQ
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Which HPLC column internal diameter should I use for LCMS - FAQ
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Which membrane filter disc is best to use with aqueous buffers - FAQ
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Which membrane is best for aqueous buffers when using syringe filters - FAQ
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Which solution from the HSQ Kit do I use for injector carry over testing - FAQ
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Which vial type should I use for a low volume sample study of proteins - FAQ
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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Why am I seeing multiple peaks in HPLC runs of fructose - Tips & Suggestions
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Why am I seeing two peaks for quaternium15 Dowicil-75 in LCMS - FAQ
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Why am I seeing two peaks for quaternium-15 or Dowicil-75 in my LCMS data - FAQ
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Why are Aqueous Normal Phase ANP HPLC methods better to use than HILIC - FAQ
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Why are environmental conditions such as temperature relative humidity that are not specified on the CofA for the UV Calibration Kit - FAQ
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Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC - FAQ
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Why are pipettors not recommended for dispensing non aqueous liquids accurately and precisely - FAQ
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Why are the Cogent Silica-C columns more stable than other silica columns - FAQ
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Why do I have higher pressure at a lower flow rate in my HPLC runs - FAQ
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Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column - FAQ
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Why do some HPLC methods use low pH buffers to separate acidic compounds - FAQ
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Why does the metal content of a silica particle contribute to the peak shape of my chromatogram in HPLC - FAQ
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Why is filtering of water samples very slow when using PTFE membrane filters - FAQ
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Why is isopropanol rarely used as a solvent in HPLC or LCMS methods - FAQ
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Why should I use your mobile phase delivery bottle caps for HPLC bottles - FAQ
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Why use Ammonium Acetate in ANP and LCMS - Tips & Suggestions
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Why would I want to use an HPLC column that is not end capped - FAQ
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Why you should use temperature control with the UDC-Cholesterol column - Tips & Suggestions
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Why your lab should adopt TYPE-C HPLC technology - White Paper
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Wikipedia reference for use of pipets with organic solvents - Primer
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Will a different guard column hardware system different from the Cogent system affect my results when using a Cogent column - FAQ
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Will acid washing autosampler vials have consequences or affect my chromatography - FAQ
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Will different guard columns packed by different column packers be equivalent - INTERNAL ONLY
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Will I protect HPLC columns from samples that get permanently adsorbed to the column by using a syringe filter - FAQ
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Will phosphates precipitate in an HPLC column - FAQ
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Will the FDA accept the Excel software in the HSQ Kit as valid - FAQ
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Will using the Cogent Column Filters change my current HPLC results - FAQ
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Working with ammonium acetate and LCMS requires attention to cleaning - Tips & Suggestions
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