Category: Mobile Phase Suggestions
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10mM Ammonium Acetate Buffer 90:10 in Acetonitrile Preparation for HPLC and LCMS - Tips & Suggestions
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Add 10 micro molar EDTA to Mobile Phase for Better Peak Shapes - Tips & Suggestions
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Ammonium Acetate or Triethylamine TEA Use with Cogent HPLC Columns - Tips & Suggestions
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Avoid microbial growth in HPLC mobile phases - How to
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Avoid Spurious Peaks from Nylon Syringe Filters with DI Water Using Electrochemical Detection - Tips & Suggestions
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Better separation of two peaks for Reversed Phase RP HPLC - Tips & Suggestions
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Buffer Concentration can Affect ANP Retention of Acids in HPLC Analyses - Tips & Suggestions
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Can I use Acetone as a mobile phase solvent in Aqueous Normal Phase ANP - FAQ
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Can I Use Benzene as a Mobile Phase Solvent in HPLC - FAQ
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Can I use Phosphoric Acid or Sodium Phosphate with the Cogent Diamond Hydride HPLC Column - FAQ
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Can I use triethylamine TEA with Cogent Diamond Hydride columns - FAQ
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Can I Use Trifluoroacetic Acid TFA to Improve the Performance in LCMS - FAQ
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Can you inject highly acidic samples on to Cogent TYPE-C Silica HPLC columns - FAQ
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Choose a Suitable Mobile Phase Additive for Negative Ionization Mode in LCMS - Tips & Suggestions
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Cocaine & Metabolite Ecognin Separation Column Suggestions - Tips & Suggestions
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Considerations for keeping gases dissolved in solution when using sparging stones - Tips & Suggestions
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Contaminants including PFAS leaching into an analysis - Tips & Suggestions
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Developing a Bromhexine HPLC method - Tips & Suggestions
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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For Sugars Analyzed by LCMS Sodium Acetate is recommended for Sensitivity of Na Adducts - Tips & Suggestions
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How does ammonium fluoride affect chromatography using Cogent TYPE-C HPLC columns - FAQ
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How much volume can the 47mm HPLC membrane filters process - FAQ
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How to handle sparging stones to avoid contamination of mobile phases - Tips & Suggestions
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How to keep the Cogent Diamond Hydride column clean when using blood as a sample - Tips & Suggestions
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If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
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If I do not remove gas from my mobile phase solvents what are the consequences - FAQ
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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
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LCMS Challenges with HILIC Mobile Phases from Salt Buildup and Ion Suppression Explained - Tips & Suggestions
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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My retention times are changing after storing my buffer and what could be causing this and tips to help avoid issues - FAQ
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Negative Baselines Observed in HPLC Runs at 214 nm - Tips & Suggestions
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Obtain a flat baseline in a gradient method without using blank subtraction - How to
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pH Tanges of Common Buffers Used in HPLC - HPLC Primer
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Positive or Negative ion mode Salt Suggestions for LC-MS Method Development - Tips & Sugeestions
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Pre mix your diluent and mobile phase in sample preparation for HPLC - Tips & Suggestions
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Prepare a 16mM Ammonium Acetate Buffer 90:10 in Acetonitrile for HPLC - Tips & Suggestions
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Restoring the Initial Method Results Analyzing AMP ADP ATP UDP and GPT in Biological Extracts - Troubleshooting
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Simple Sugars Methods using Triethylamine TEA in Aqueous Normal Phase ANP Methods - Tips & Suggestions
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Suggestion for separation of phenylephrine HCl acetaminophen and dextromethorphan - Tips & Suggestions
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Techniques to prepare a 0.05% solution from a tablet for HPLC injection - How to
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Tip for working with plasma samples and Cogent TYPE-C HPLC columns - Tips & Suggestions
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To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
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Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run - Tips & Suggestions
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Use Bubble Point to Filter Water Through Hydrophobic Membranes - HPLC Primer
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Using methanol instead of acetonitrile in an HPLC method should be done with caution - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What is the durability of the SOLVFIL when used with standard mobile phases and buffered mobile phases - FAQ
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What is the maximum salt concentration to use with Cogent TYPE-C HPLC columns with HPLC or LCMS - FAQ
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What is the pH Range of 316 Stainless Steel Tubing Fittings and Sparging Stones - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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What is the solvent strength order in Aqueous Normal Phase ANP HPLC - FAQ
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Which Membrane Filter is Best to Use with Aqueous Fuffers - FAQ
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Why do I have higher pressure at a lower flow rate in my HPLC runs - FAQ
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Why do I have lower than normal back pressure with Cogent Diamond Hydride HPLC columns - FAQ
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Will phosphates precipitate in an HPLC column - PRIMER
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Will TFA permanently alter retention / selectivity of the Cogent TYPE-C silica columns - FAQ
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