Category: Mobile Phase Suggestions
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Avoid Spurious Peaks from Nylon Syringe Filters with DI Water Using Electrochemical Detection - Tips & Suggestions
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Buffer Concentration can Affect ANP Retention of Acids in HPLC Analyses - Tips & Suggestions
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Can I use phosphoric acid or sodium phosphate with the Cogent Diamond Hydride HPLC column - FAQ
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Considerations for keeping gases dissolved in solution when using sparging stones - Tips & Suggestions
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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For sugars analyzed by LCMS sodium acetate is recommended for sensitivity of Na adducts - Tips & Suggestions
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High pressure develops after flushing a Cogent Diamond Hydride column with acetonitrile cause and solution - FAQ
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
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How to handle sparging stones to avoid contamination of mobile phases - Tips & Suggestions
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How to keep the Cogent Diamond Hydride column clean when using blood as a sample - Tips & Suggestions
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How to prepare 10mM ammonium acetate buffer 90:10 in acetonitrile for HPLC and LCMS - Tips & Suggestions
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How to prepare 16mM ammonium acetate buffer 90:10 in acetonitrile for HPLC - Tips & Suggestions
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If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
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Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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My retention times are changing after storing my buffer and what could be causing this and tips to help avoid issues - FAQ
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Restoring the initial results with a method for analyzing AMP ADP ATP UDP and GPT in biological extracts - Tips & Suggestions
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Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run - Tips & Suggestions
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Suggestion for separation of phenylephrine HCl acetaminophen and dextromethorphan - Tips & Suggestions
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To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
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Two gradients to use for analysis of hydrophilic and or hydrophobic molecules in the same Aqueous Normal Phase ANP method - Tips & Suggestions
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Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run - Tips & Suggestions
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Using methanol instead of acetonitrile in an HPLC method should be done with caution - Tips & Suggestions
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Using triethylamine TEA in Aqueous Normal Phase ANP methods for simple sugars - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What is the durability of the SOLVFIL when used with standard mobile phases and buffered mobile phases - FAQ
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What is the maximum salt concentration to use with Cogent TYPE-C HPLC columns with HPLC or LCMS - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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Which acid or base additive should I choose for positive or negative ion mode in LC-MS - FAQ
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Which column do you suggest for Cocaine & metabolite Ecognin separation - Tips & Suggestions
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