Mobile Phase Preparation, Buffers & Solvent Handling
/
/
/
Explore practical guidance for preparing HPLC mobile phases, selecting buffers and additives, handling solvents, controlling contamination, managing salts, and optimizing LC-MS compatibility. Learn best practices for reliable chromatography performance and reproducible analytical results.
10mM Ammonium Acetate Buffer 90:10 in Acetonitrile Preparation for HPLC and LCMS - Tips & Suggestions
Read More
Read More
Ammonium Fluoride Effect on Chromatography Using Cogent TYPE-C HPLC Columns - Tech Information
Read More
Read More
Avoid Spurious Peaks from Nylon Syringe Filters When Using Electrochemical Detection - Tech Information
Read More
Read More
Buffer Concentration Effects on Acid Retention in HILIC Methods Using TYPE-C™ Columns - Tech Information
Read More
Read More
Choose a Suitable Mobile Phase Additive for Negative Ionization Mode in LCMS - Tips & Suggestions
Read More
Read More
Cocaine and Polar Metabolites ( Ecgonine) Separation using LC‑MS / MS - Tips and Suggestions
Read More
Read More
For Sugars Analyzed by LCMS Sodium Acetate is recommended for Sensitivity of Na Adducts - Tips & Suggestions
Read More
Read More
Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
Read More
Read More
Maximum Salt Concentration Recommendations for Cogent TYPE-C HPLC Columns - Tech Information
Read More
Read More
My retention times are changing after storing my buffer and what could be causing this and tips to help avoid issues - FAQ
Read More
Read More
Phosphoric Acid and Sodium Phosphate Use with Cogent Diamond Hydride Columns - Tech Information
Read More
Read More
Positive and Negative Ion Mode Mobile Phase Additives for LC-MS Method Development - Tech Information
Read More
Read More
Prepare a 16mM Ammonium Acetate Buffer 90:10 in Acetonitrile for HPLC - Tips & Suggestions
Read More
Read More
Restoring the Initial Method Results Analyzing AMP ADP ATP UDP and GPT in Biological Extracts - Troubleshooting
Read More
Read More
Selecting the Optimal Gradient Direction for Mixed-Polarity Compounds Using Cogent TYPE-C Columns - Tech Information
Read More
Read More
Solvent Strength and Mobile Phase Selection for HILIC Methods Using Cogent TYPE-C Columns - Tech Information
Read More
Read More
To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
Read More
Read More
Using Basic Sample Diluents with HILIC Methods on Silica-Based HPLC Columns - Tech Information
Read More
Read More