Category: ANP Aqueous Normal Phase Articles
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Analyze phosphorylated compounds with the Cogent Diamond Hydride column - Tips & Suggestions
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Analyzing metformin in plasma samples how do I obtain reproducible results and peak shapes - FAQ
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Aqueous Normal Phase ANP and how can it is useful for polar compounds - Tech Information
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Aqueous Normal Phase ANP ballistic gradients compared to same in HILIC - Tips & Suggestions
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Aqueous Normal Phase ANP defined & compared to Reversed Phase & Normal Phase definitions HPLC
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Aqueous Normal Phase ANP HPLC retention mechanism elucidated - Tech Information
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Aqueous Normal Phase ANP HPLC what is it - FAQ
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Aqueous Normal Phase ANP solvent strength order - FAQ
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Are there HPLC columns that could be used to separate both polar and nonpolar compounds in a single run - FAQ
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Better methods than HILIC in HPLC and LCMS - Tips & Suggestions
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Buffer concentration can affect ANP retention of acids in HPLC Analyses - Tips & Suggestions
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Can Aqueous Normal Phase ANP methods be used to separate steroids - FAQ
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Can I use Acetone as a mobile phase solvent in Aqueous Normal Phase ANP - FAQ
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Can I use the Cogent Diamond Hydride column without mobile phase additives like formic acid - FAQ
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Can Reversed Phase and ANP mechanisms operate with the same effect at the same time - FAQ
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Changing column ID means changing your gradient - Tips & Suggestions
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Choose an appropriate guard column when using a Cogent TYPE-C HPLC column - How To
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Cogent silica hydride HPLC columns by order of hydrophobicity - Tips & Suggestions
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Converting Cogent TYPE-C columns to normal phase after use in reversed phase - How To
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Do Tanaka Plots for HPLC apply to columns used with Aqueous Normal Phase ANP methods - FAQ
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Do the Cogent Silica-C columns contain any carbon - FAQ
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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Helpful HPLC columns you may not know about - White Paper
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How can I fix poor peak shape for phosphorylated compounds in aqueous normal phase ANP methods - FAQ
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How can I improve accuracy and precision when separating polar compounds by HPLC using Aqueous Normal Phase ANP - FAQ
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How does ammonium fluoride affect HPLC and LCMS data if using Aqueous Normal Phase ANP methods - FAQ
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How does efficiency in Aqueous Normal Phase ANP compare to HILIC in HPLC - FAQ
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How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
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How to eliminate retention time drift in LCMS with Aqueous Normal Phase ANP methods - Tips & Suggestions
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How to fix a saw tooth pattern in the UV baseline - Tips & Suggestions
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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Improvement in signal to noise in LCMS with an ANP method - Tips & Suggestions
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Improving peak shapes in an HPLC method in aqueous normal phase ANP - Tips & Suggestions
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Injection Technique for Aqueous Samples using Cogent TYPE-C Columns in ANP - Tips & Suggestions
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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
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LFER studies compared to Log D data in describing the Aqueous Normal Phase ANP retention - Tips & Suggestions
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Method development tip for maleic hydrazide - Tips & Suggestions
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Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
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Negative Baselines in HPLC at 214nm - Tips & Suggestions
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No need to use a high pH mobile phases for compounds with a high pKa - Tips & Suggestions
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Obtain a flat baseline in a gradient method without using blank subtraction - How to
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Percentage of acid needed in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Polar and non polar peptides analyzed by LCMS with an Aqueous Normal Phase ANP method - Tips & Suggestions
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Purge metals from HPLC system using EDTA or medronic acid - How To
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Sample concentration changes can effect retention time in HPLC - Primer
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Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP - FAQ
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Solving baseline problems in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Store and condition Cogent TYPE-C HPLC columns - How To
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Suggestions for HPLC method development with silica hydride columns - Tips & Suggestions
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The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase - Tips & Suggestions
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Tip for working with plasma samples and Cogent TYPE-C HPLC columns - Tips & Suggestions
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Two gradients to use for analysis of hydrophilic and or hydrophobic molecules in the same Aqueous Normal Phase ANP method - Tips & Suggestions
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Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run - Tips & Suggestions
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Use Teflon bottles for A and B solvents in negative ion mode LCMS - Primer
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Using ammonium acetate or ammonium formate in the Diamond Hydride - Tips & Suggestions
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Using ammonium acetate or formate in an acetonitrile based HPLC mobile phase for Aqueous Normal Phase ANP - Tips & Suggestions
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Using ammonium acetate or triethylamine TEA with Cogent HPLC columns - Tips & Suggestions
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Using an Aqueous Normal Phase ANP method will sodium ions interfere with succinate when analyzing sodium succinate - FAQ
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Using Aqueous Normal Phase ANP to your advantage - White Paper
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Using triethylamine TEA in Aqueous Normal Phase ANP methods for simple sugars - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies - FAQ
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What are your definitions of normal phase reversed phase and ANP in HPLC - FAQ
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What does Post Time mean in an LCMS or HPLC gradient method - FAQ
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What effect does high injection volume have on column performance in Aqueous Normal Phase ANP HPLC- FAQ
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What is the maximum salt concentration to use with Cogent TYPE-C HPLC columns with HPLC or LCMS - FAQ
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What is the purpose of different bonded phases using Cogent TYPE-C Silica - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time - FAQ
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When and why use Teflon bottles for your mobile phase reservoirs - FAQ
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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Why are Aqueous Normal Phase ANP HPLC methods better to use than HILIC - FAQ
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Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC - FAQ
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Why do I have lower than normal back pressure with Cogent Diamond Hydride HPLC columns - FAQ
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Why doesn't triphenylamine a tertiary amine retain in Aqueous Normal Phase ANP - FAQ
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Why use Ammonium Acetate in ANP and LCMS - Tips & Suggestions
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