HPLC Learning Center & Technical Resources
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Explore key basic HPLC fundamentals, tips, and practical guidance to help you understand methods, troubleshoot issues, and learn essential concepts.
Activity of a Stationary Phase in Chromatography Definition and Impact on HPLC Separations - Tech Information
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Adsorption vs. Absorption: Understanding the Difference in Chromatography and HPLC - Tech Information
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Amide vs Amino HPLC Columns Differences in Stability Selectivity and Performance - Tech Information
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Ammonium Acetate and Ammonium Formate in Acetonitrile Rich HILIC Mobile Phases - Tech Information
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Ammonium Acetate in LC-MS Methods Preventing Source Contamination and Signal Loss - Tech Information
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Benzene as an HPLC Mobile Phase Solvent: Compatibility, Limitations, and Safety Considerations Tech Information
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Broad and Asymmetrical Peak Troubleshooting in Reversed-Phase LC-MS Methods - Tech Information
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Calculating Theoretical Plates N Using Peak Width at Half Height in HPLC - Tech Information
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Cast vs Skived PTFE in Headspace Septa and Their Impact on Quantitative Results - Tech Information
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Choosing the Right Autosampler Vial for HPLC, UHPLC and LC-MS Applications - Tech Information
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Cogent Phenyl Hydride Bonding Technology and Stationary Phase Structure - Tech Information
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Column Cleaning and Regeneration with Gradient Cycling Techniques for HPLC - Tech Information
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Column Void Volume and Capacity Factor Calculations for HPLC Method Transfer - Tech Information
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Comparing Wet Chemical Assays and HPLC Assays for Analytical Reliability - Tech Information
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Concentration of an Impurity in HPLC using the Relative Response Factor RRF - Tech Information
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Converting 0.1% w/v Ammonium Acetate to Millimolar Concentration for HPLC and LC-MS - Tech Information
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Creatine and Creatinine Analysis in EDTA Plasma Using HPLC and HILIC Methods - Tech Information
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Differences Between 33 Expansion and 51 Expansion Glass for Autosampler Vials - Tech Information
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Differences Between Assay and Purity Testing in Pharmaceutical Analysis - Tech Information
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Direct Silicon to Carbon Bonds on Cogent TYPE-C HPLC HPLC Columns Produce Longer Column Lifetimes - Tech Information
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Dwell Volume Effects on Gradient Retention and Method Transfer in HPLC Systems - Tech Information
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Effective Filtration Area and Burst Pressure Specifications for AQ Syringe Filters - Tech Information
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Effects of Washing and Reusing Autosampler Vials on Sample Recovery and Contamination Risk - Tech Information
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Evaporation Studies Autosampler Cap Selection and Solvent - Tech Information- Tech Information
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Extra Column Volume calculation using the Chemical Solutions Qualification Kit software - Tech Information
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Extractables and Leachables in Chromatography and Pharmaceutical Packaging - Tech Information
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FDA Approval is not Available for RSA Vials or for Use in Clinical Resting - Tech Information
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Fluoropolymer Tubing Materials for Laboratories PFA PTFE FEP and ETFE Explained - Tech Information
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Formic Acid Improves Peak Shapes Retention and Carryover for Polar Compounds - Tech Information
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Gradient Method Adjustments When Changing HPLC Column Internal Diameter - Tech Information
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HILIC Methods Using Cogent TYPE-C Columns Compared to Conventional HILIC Columns - Tech Information
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How Tight Should an Autosampler Vial Cap Be to Prevent Septa Push-Through During Injection - Tech Information
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HPLC and LC-MS Method Validation Fundamentals for Regulated Laboratories - Tech Information
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Hydrophobicity and Column Selection Guide for Cogent TYPE-C HPLC Columns - Tech Information
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Improving Citric Acid Retention and Peak Shape Reproducibility in LC-MS Analysis - Tech Information
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Improving LC-MS Sensitivity with HILIC Methods for Polar Compound Analysis - Tech Information
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Integrating Chromatographic Peaks with Shoulders Peak to Valley Evaluation - Tech Information
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Ion Content Differences Between Clear and Amber Borosilicate Glass Vials – Tech Information
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Ion Suppression Strategy in LC-MS When Ion-Pair Reagents Are Traditionally Required - Tech Information
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Limits of Detection LOD and Limits of Quantification LOQ in Chromatographic Method Validation - Tech Information
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Linearity Assessment for HPLC Method Validation and Quantitative Analysis - Tech Information
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Maximum Operating Pressure Ratings for Cogent HPLC Columns by Particle Size - Tech Information
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Metal-Free Coated Stainless Steel Hardware and Frits for HPLC Applications - Tech Information
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Moisture Control Considerations for Autosampler Vials and Caps Used in HPLC and GC - Tech Information
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MRQ and Max Recovery Autosampler Vials Functional Differences and Selection Considerations - Tech Information
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pKa pKb and pH Considerations for HPLC Mobile Phases and Sample Diluents - Tech Information
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Platinum Catalyst Residue Evaluation on Cogent TYPE C Silica Stationary Phases - Tech Information
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Polar Compounds Often Show Poor Retention on Traditional Reversed Phase Columns - Tech Information
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Positive and Negative Ion Mode Mobile Phase Additives for LC-MS Method Development - Tech Information
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Preparing 10 mM Ammonium Acetate Mobile Phases in High-Acetonitrile HILIC Methods - Tech Information
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Preparing Premixed Diluents to Improve Accuracy in HPLC Sample Preparation - Tech Information
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Pressure Increases Following Sample and Blank Injections in HPLC Systems - Tech Information
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Proper Orientation of Butyl/PTFE Septa in Autosampler and Headspace Vial Caps - Tech Information
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Relative Response Factor RRF and Correction Factors in HPLC Quantitation - Tech Information
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Retaining Polar Compounds with Cogent Bidentate C18 Columns Reversed Phase and HILIC Considerations - Tech Information
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Retention of Ionized Polar Compounds in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Retention Time Variability Caused by Mobile Phase Mixing Inconsistencies in HPLC Systems - Tech Information
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Retention Times Changes with Mobile Phase Storage Effects on Retention Time and Peak Shape in HPLC Methods - Tech Information
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RSA Autosampler Vials Versus Silanized Vials for Pharmaceutical and LC-MS Applications - Tech Information
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Sensitivity and the Impact of Column Related Contamination in LC-MS Methods - Tech Information
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Silica or Silica‑C Guard Columns with Analytical Columns of Different Phases - Tech Information
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Silicone Rubber Purity and Curing Methods Used in Autosampler Vial Septa - Tech Information
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Sodium and Ammonium Adduct Formation for Improved Sugar Detection in LC-MS - Tech Information
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Solid-State NMR Structure Confirmation of Cogent Bidentate C8 Stationary Phases - Tech Information
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Some HPLC Fittings May Not Require Ferrules and Understanding Integrated and Separate Ferrule Designs - Tech Informaton
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Stability of Cogent Silica-C Columns in Normal Phase and HILIC Applications - Tech Information
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Stainless Steel Ferrules and Stainless Steel and PEEK Hybrid Ferrules Differences - Tech Information
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Starting Flow Rate Guidelines for Common HPLC Column Internal Diameters - Tech Information
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Static Mixer Placement for Improved HPLC Mobile Phase Mixing and Detector Stability - Tech Information
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Storage Conditioning and Solvent Transition Guidelines for Cogent TYPE-C HPLC Columns - Tech Information
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Surface Charge Characteristics of Cogent TYPE C Silica and Their Effect on Basic Compound Retention - Tech Information
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Temperature Range of 316 Stainless Steel Laboratory Products That are Useful - Tech Information
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Tubing Internal Diameter and Its Impact on HPLC Pressure and Performance - Tech Information
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TYPE-C Silica Is Made with High Purity Silica and Has a Low Metal Content - Tech Information
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Understanding 45 Shore A and 55 Shore A Hardness Ratings in Autosampler Vial Septa - Tech Information
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Understanding Nano LC Capillary LC and Micro LC Column Dimensions and Flow Rates - Tech Information
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Understanding Normal Phase Reversed Phase and HILIC Retention Modes with TYPE-C Columns - Tech Information
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Understanding Polar Compound Retention Why LFER Models Often Provide More Insight Than Log D - Tech Information
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Understanding PSI, PSIG, and PSIA Pressure Measurements in HPLC Systems and Fluid Path Components - Tech Information
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Understanding the Impact of Extra Column Volume on HPLC Method Performance - Tech Information
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Using Basic Sample Diluents with HILIC Methods on Silica-Based HPLC Columns - Tech Information
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USP Chapter 621 Allowed Adjustments to Chromatographic Methods and Method Modernization - Tech Information
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Wavelength Precision and Absorbance Precision Testing in UV-Vis Qualification Software - Tech Information
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