SINCE 1992
 
 
 

No Knowledge Base information

No Application Notes information

Our goal is to provide the best Customer Experience possible. If you cannot find what you are looking for, have any questions or would like to give us a suggestion please use any of the following ways to contact us directly. We would love to hear from you.

We are always ready to lend any support we can. Our experienced scientists and professionals are ready to assist you with any technical support questions. Every question is considered a good question and we will work with you on a solution as fast as possible.

Separate Halogenated from Non‑Halogenated Compounds

USP® L43 | Scalable from Analytical to Preparative HPLC

Cogent RP PFP™ (Pentafluorophenyl) HPLC columns are specifically designed to deliver enhanced selectivity for halogenated compounds, making them an excellent choice when traditional C18 or phenyl phases do not provide sufficient resolution. These columns are widely used to separate halogenated from non‑halogenated aromatic compounds, a key challenge in pharmaceutical, chemical, and environmental analyses.

Cogent RP PFP™ columns are classified under USP® L43 and are available in formats suitable for analytical, semi‑prep, and preparative HPLC, supporting seamless scale‑up.

Why Choose a PFP (Pentafluorophenyl) Phase?

Pentafluorophenyl stationary phases provide multiple retention mechanisms beyond simple hydrophobic interactions. In addition to reversed‑phase behavior, PFP columns offer:

  • π–π interactions

  • Dipole–dipole interactions

  • Enhanced selectivity for halogens and electronegative substituents

This combination makes PFP columns particularly effective for resolving structurally similar aromatic compounds, positional isomers, and halogenated species that co‑elute on conventional phases.

Cogent RP PFP™ Column Construction

Cogent RP PFP™ columns are manufactured using world‑class, high‑purity silica and a bonding technology refined over many years, resulting in a highly stable and reproducible stationary phase.

Key construction features include:

  • Pentafluorophenyl (PFP) bonded phase

  • Fully end‑capped surface for reduced secondary interactions

  • Available in 60 Å and 100 Å pore sizes

  • Offered in a wide range of particle sizes

  • Designed for consistent performance and long column lifetime

Compared to simple phenyl phases, Cogent RP PFP™ columns often provide improved selectivity and peak shape, particularly for halogenated analytes.

Key Features and Benefits

  • Designed to separate halogenated from non‑halogenated compounds

  • USP® L43 classified stationary phase

  • Enhanced selectivity for aromatic and halogenated analytes

  • Fully end‑capped for improved peak shape

  • Manufactured with high‑purity silica

  • Available in multiple particle sizes

  • 60 Å and 100 Å pore size options

  • Scalable from analytical to preparative HPLC

Typical Applications

  • Halogenated pharmaceuticals and intermediates

  • Aromatic and substituted aromatic compounds

  • Positional isomers

  • Impurity profiling

  • Pharmaceutical and chemical separations

  • Analytical and preparative chromatography

Additional Resources

Column Protection Recommendation

Important: MICROSOLV technical support strongly recommends using the Cogent Column Filter, which is included with each column, whenever possible. Column filters help protect against particulates, extend column lifetime, and maintain consistent chromatographic performance.

👉Learn more about the Cogent Column Filter for HPLC column protection

Clear All Selections Clear All Selections Share mdi-content-copy
{{ pids.length }} PRODUCTS MATCHING
Toggle to view Images Toggle to view Table

© Copyright 2026. MICROSOLV. All Rights Reserved. Website & Hosting by BlueTone Media