Calculating theoretical plates N at half height - How To
April 14, 2020
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The theoretical plate number N0.5 can be calculated from a chromatogram using the equation below:
N0.5 = 5.54 (tR/w0.5)2
Where tR is retention time and w0.5 is the peak width at half its height. Remember to use the same units (minutes or seconds) for both values (tR and w0.5).
