Category: Frequently Asked Questions
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Analyte retention decreases with mobile phase water content using the Cogent Bidentate C18 HPLC column has anything happened to the column - Tech Information
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Aqueous Normal Phase ANP ballistic gradients compared to same in HILIC - Tips & Suggestions
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Aqueous Normal Phase ANP defined & compared to Reversed Phase & Normal Phase definitions HPLC
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Are septa in autosampler vial caps self sealing inert and compatible with organic solvents - FAQ
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Are the Chemical Solutions kits compliant with the latest USP-NF chapter revisions for 857 and 1857 - FAQ
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Are the MicroSolv AQ brand of syringe filter membranes supported by extra membranes in the plastic housing - FAQ
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Are there any solvents that I cannot use with the knitted reactor coils KRC and knitted delay tubes KDT - FAQ
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Are there HPLC columns that could be used to separate both polar and nonpolar compounds in a single run - FAQ
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Are there inherent problems with autosampler vials that have a filter built into the cap - FAQ
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At which pH will a method be optimized with Zero Flow and Controlled Flow capillaries - FAQ
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Autosampler vials sold by MicroSolv will fit properly in your instruments - Tech Information
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Can amber or clear polypropylene vials be sterilized with autoclaving or ethylene oxide - FAQ
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Can an autosampler needle pierce the Zone Free sealing film and will it reseal afterwards - FAQ
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Can I choose HPLC mobile phase percentages other than those used in the HSQ Spreadsheet - FAQ
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Can I sterilize screw caps for autosampler vials that have silicone rubber with PTFE septa - FAQ
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Can I use a laser to make a detection window for the Controlled Flow and Zero Flow capillaries - FAQ
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Can I use a PEEK finger tight fitting on stainless steel tubing - Can I use a SS fitting on PEEK tubing in HPLC - FAQ
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Can I use heat to remove the polyimide coating on Zero Flow capillaries without damaging the inner coating - FAQ
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Can I use the certified HPLC column supplied in the HSQ Kit for UHPLC performance qualification - FAQ
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Can I use the holmium oxide solution in a UV instrument that calls for Starna or NIST cuvettes - FAQ
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Can I use the MicroSolv Window Maker to remove polyimide from wires or solid core glass fiber - FAQ
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Can I use trifluoroacetic acid TFA to improve the chromatographic performance in LCMS - FAQ
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Can I use vial 95025-VN-05C with crimp cap 95025-08-1S in an oven at 105C for approximately 1 month - FAQ
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Can the HSQ or PQ qualification kits be used with Waters equipment and Empower chromatography data software - FAQ
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Can the MicoSolv Window Maker be used to remove the polyimide from the tips of capillaries - FAQ
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Can the U-2D Micro-Sample management system be used in SPE manifold for 96-well plates - FAQ
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Can the Window Maker heating modules be used with either US or European voltage units - FAQ
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Could your PEEK tubing have contaminants via manufacturing processes from previous extrusion runs - FAQ
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Differences between the linearity solutions in the HSQ qualification kit - Tech Information
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Difficulties with wavelength accuracy readings while performing a PQ of an HPLC - Tips & Suggestions
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Do different retention times for peaks in the Resolution Test Mixture on different systems impact the integrity of my data - FAQ
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Do I need any special fittings to use the Cogent Column Filter with my current HPLC column - FAQ
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Do Tanaka Plots for HPLC apply to columns used with Aqueous Normal Phase ANP methods - FAQ
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Do the MicroSolv mobile phase delivery bottle caps have holes to insert tubing through - FAQ
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Does MicroSolv sell bared fused capillaries for CZE that are completely UV-transparent - FAQ
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Does the Hichrom brand column coupler work with the Partisil and Partisphere HPLC columns - FAQ
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Does the replaceable heating module come with the MicroSolv Window Maker when you order it or does it have to be ordered separately - FAQ
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Does the UV-vis calibration kit operate in all three ranges UV and visible and visible/NIR - FAQ
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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Ghost peaks appearing in results using a gradient with TFA which can degrade with time - Tips & Suggestions
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How are your polypropylene vials and inserts for LCMS different from those of other manufacturers - FAQ
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How can Cogent TYPE-C HPLC columns offer faster results compared to other type of silica based columns - FAQ
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How can I improve accuracy and precision when separating polar compounds by HPLC using Aqueous Normal Phase ANP - FAQ
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How can I make changes to locked cells in the Excel spreadsheet that comes with the HSQ qualification kit - FAQ
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How do RSA autosampler vials compare against silanized vials for pharma applications - FAQ
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How does the shorter column supplied in the HSQ kit affect the data compared to the longer column from the PQ Kit - FAQ
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How long will it take for chloroform to degrade the plastic safety coating on the mobile phase bottles if the bottle is broken during a fall - FAQ
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How often do I need to do the conditioning procedure for a new Diamond Hydride column - FAQ
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How similar fittings for chromatography can differ from different brands in HPLC - Tech Information
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How tight should an autosampler vial cap be to prevent push through of the septa during injection - FAQ
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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I currently use a glass filter why should I use the HSQ Kit solutions instead for qualification - FAQ
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
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I see extra peaks in my chromatography when I am not using the same filter - Tips & Suggestions
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If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
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If I use a buffer at pH 9 at 30 to 35C with the Zero Flow capillaries what lifetime should I expect from them - FAQ
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In a cap that contains a butyl rubber with a PTFE septa which direction does the PTFE membrane face - FAQ
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In order to prevent gas venting from an autosampler vial cap should I use snap or crimp or a screw cap - FAQ
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Is it possible to make a window anywhere on the capillary using the MicroSolv Window Maker - FAQ
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Is the conditioning of the Controlled Flow and Zero CE capillaries the same as with a bare fused silica capillary - FAQ
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Is the Excel template included in the Chemical Solutions Qualification kits CFR Part 21.11 compliant - FAQ
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Is the Holmium Oxide Solution intended as a stand alone product or solely as a replacement solution for the HSQ Kit - FAQ
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Is the power cable supplied with the MicroSolv Window Maker or is it a separate accessory - FAQ
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Is there a difference in chromatography performance between clear and amber RSA vials - FAQ
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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My retention times are changing after storing my buffer and what could be causing this and tips to help avoid issues - FAQ
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Protecting HPLC analytical columns and when to use guard columns vs Cogent Column Filters - Tips & Suggestions
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Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP - FAQ
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Silicone rubber in septa for autosampler vials vary in the way it is made - Tech Information
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ
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The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase - Tips & Suggestions
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To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
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Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run - Tips & Suggestions
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Using methanol instead of acetonitrile in an HPLC method should be done with caution - Tips & Suggestions
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Using the wavelength calibration solution holmium oxide for HPLC UV detector performance qualification - Tips & Suggestions
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Vials are listed as 12 x 32mm but my autosampler accepts a maximum diameter of 11.8mm will they fit - FAQ
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What are some of the advantages of using Cogent TYPE-C prep and semi-prep HPLC columns - FAQ
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What are the differences between 33 and 51 expansion glass and which should I choose - FAQ
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What are the size and materials of the mobile phase delivery bottle caps and O-rings - FAQ
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What are the temperature specifications of the autosampler vial storage box and cap for 4ml vials - FAQ
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What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
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What is the advantage or disadvantage of using Certified autosampler vials compared to MicroSolv vials - FAQ
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What is the chemical compatibility of the Chemical Resistant Sealing Mats for U-2D Micro-Sample Management System - FAQ
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What is the chemical structure of the ligand in the Cogent DIOL HPLC stationary phase - FAQ
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What is the compatibility of Cogent TYPE-C Silica HPLC columns with UHPLC instruments - FAQ
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What is the difference between ferrules made of stainless steel vs stainless steel and PEEK hybrids - FAQ
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What is the difference between precision point and conical point autosampler vial inserts - FAQ
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What is the difference between the Beta Tools and other similar pliers on the market - FAQ
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What is the difference between weak and strong cation exchange for Ion Exchange columns - FAQ
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What is the durability of the SOLVFIL when used with standard mobile phases and buffered mobile phases - FAQ
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What is the elution order for analytes in the Resolution Test Mixture supplied in the HSQ kit - Tech Information
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What is the inside diameter of the opening of your Luer Lock syringes catalog number 58901-S - FAQ
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What is the maximum concentration of ammonium fluoride to use in a mobile phase to avoid column damage - Tips & Suggestions
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What is the maximum loading capacity mass injection volume that can be used for a semi prep Cogent TYPE-C HPLC column - FAQ
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What is the maximum salt concentration to use with Cogent TYPE-C HPLC columns with HPLC or LCMS - FAQ
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What is the pH range of 316 stainless steel laboratory tubing fittings and sparging stones - FAQ
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What is the purpose of the Resolution Test Mixture supplied in your Qualification Kits - FAQ
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What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods - FAQ
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What is the recommended sparging stone pore size to use for carbonating applications - FAQ
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What makes non-coated Mobile Phase Reservoir Bottles suitable for use in various temperature environments - FAQ
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What maximum flow rate can I use for a PTFE 10m 0.75mm ID Knitted Reactor Coil KRC with isopropanol - FAQ
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What precautions are used in the assembly of your vial and cap kits to prevent contamination - FAQ
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What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time - FAQ
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What tubing is suitable for use in a knitted reactor coil KRC system utilizing isopropanol or concentrated HCl or concentrated NaOH or copper or luminol - FAQ
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What type of tubing do I use to connect the guard column holder to analytical columns - FAQ
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What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column - FAQ
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When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative - Primer
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Which column coupler do you recommend for pairing HPLC columns with guard columns- Tech Information
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Which crimp cap is most commonly used for residual solvent testing by the pharmaceutical industry - FAQ
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Which crimp caps and septa are suitable for use with Solid Phase Micro Extraction SPME vials - FAQ
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Which HPLC column ID dimensions are the Hichrom brand guard column holders cat no 82000-GH compatible with - FAQ
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Which type of vial do you recommend to use with the U-2D Micro Sample Management inserts - FAQ
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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Why are environmental conditions such as temperature relative humidity that are not specified on the CofA for the UV Calibration Kit - FAQ
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Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC - FAQ
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Why are pipettors not recommended for dispensing non aqueous liquids accurately and precisely - FAQ
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Why are the same size autosampler vials listed as 2mL 1.8mL or 1.5mL by different suppliers - FAQ
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Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column - FAQ
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Why does the color of the Holmium Oxide solution for HPLC performance qualification look different than before - FAQ
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Why is it not recommended to use FEP tubing above 80°C when the melting point is much higher - FAQ
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Why you should use temperature control with the UDC-Cholesterol column - Tips & Suggestions
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Will I protect HPLC columns from samples that get permanently adsorbed to the column by using a syringe filter - FAQ
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Will I see any differences in my HPLC chromatography if I change to AQ syringe filters - FAQ
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