Category: 2.o™ HPLC Columns FAQ
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Analyte retention decreases with mobile phase water content using the Cogent Bidentate C18 HPLC column has anything happened to the column - Tech Information
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Analyze phosphorylated compounds with the Cogent Diamond Hydride column - Tips & Suggestions
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Aqueous Normal Phase ANP and how can it is useful for polar compounds - Tech Information
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Aqueous Normal Phase ANP ballistic gradients compared to same in HILIC - Tips & Suggestions
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Aqueous Normal Phase ANP HPLC what is it - FAQ
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Aqueous Normal Phase ANP solvent strength order - FAQ
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Are Cogent 2.o HPLC columns compatible with Agilent 1290 Infinity II instruments - FAQ
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Are Cogent TYPE-C silica 2.o columns considered UHPLC phases - FAQ
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Are there HPLC columns that could be used to separate both polar and nonpolar compounds in a single run - FAQ
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Bubbling or fizzing coming from a new Cogent TYPE-C column - Tech Information
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Calculate the ligand density of a bonded phase of an HPLC column - How To
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Can Aqueous Normal Phase ANP methods be used to separate steroids - FAQ
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Can Cogent TYPE-C HPLC columns be used in supercritical fluid chromatography SFC - FAQ
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Can I use 4um guard columns with the 2.o analytical columns - FAQ
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Can I use of the Cogent Diamond Hydride HPLC column above pH 7 in HPLC - FAQ
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Can I use triethylamine TEA with Cogent Diamond Hydride columns - FAQ
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Choose an appropriate guard column when using a Cogent TYPE-C HPLC column - How To
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Cogent Diamond Hydride 2.o HPLC column retention time precision - AppNote
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Cogent silica hydride HPLC columns by order of hydrophobicity - Tips & Suggestions
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Cogent TYPE-C column end fitting hardware and dimensions - Tips & Suggestions
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Column cleaning and care in UHPLC columns - Tips & Suggestions
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Determining Efficiency of HPLC methods comparing 4um v 2.o HPLC columns - Tips & Suggestions
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Developing a Bromhexine HPLC method - Tips & Suggestions
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Do Tanaka Plots for HPLC apply to columns used with Aqueous Normal Phase ANP methods - FAQ
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Do the Cogent Silica-C columns contain any carbon - FAQ
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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Eliminate high background noise using a new Cogent HPLC column - Tips & Suggestions
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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Fosetyl aluminum analysis by Reversed Phase HPLC - Tips & Suggestions
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Helpful HPLC columns you may not know about - White Paper
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High molecular weight compounds that won't retain on Cogent Diamond Hydride column try Cogent Bidentate C8 300 - Tips & Suggestions
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How a pulse from an HPLC pump affect the column and its performance - Tech Information
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How can I restore my Diamond Hydride HPLC column after I ran out of mobile phase and air was accidently introduced into it - FAQ
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How can I separate capsaicinoids in hot sauce by HPLC - Tips & Suggestions
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How do retention and efficiency of asymmetric dimethylarginine ADMA compare using 4um vs 2.o Diamond Hydride columns - AppNote
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How much higher is the pressure generated on the Cogent 2.o columns than 4um phases - FAQ
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How often do I need to clean or wash a Cogent TYPE-C column to keep data precise - FAQ
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How often do I need to do the conditioning procedure for a new Diamond Hydride column - FAQ
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How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
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How to fix a saw tooth pattern in the UV baseline - Tips & Suggestions
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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How to improve LOD or detection limits in HPLC - Tips & Suggestions
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How to keep HPLC columns free of adsorbed contaminants - How To
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How to keep surface treated frits from biofouling - FAQ
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How to minimize HPLC column blockage from most common causes - Tips & Suggestions
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How to separate phospholipids with HPLC - Tips & Suggestions
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HPLC stationary phase can be responsible for a blocked frit- Tips & Suggestions
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I am not observing any peaks for succinic acid or alpha-ketoglutarate with the Cogent Diamond Hydride column - Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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Inconsistent peak heights obtained for glucosamine in HPLC - Tips & Suggetions
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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
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Is the Cogent Column Filter compatible with a 2.1mm ID column - FAQ
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Lower retention is observed when functional group in a compound is ionized - Tips & Suggestions
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Method development tip for maleic hydrazide - Tips & Suggestions
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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material - FAQ
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Negative Baselines in HPLC at 214nm - Tips & Suggestions
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No need to use a high pH mobile phases for compounds with a high pKa - Tips & Suggestions
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Peak area decreases with increasing flow rate - FAQ
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Polar and non polar peptides analyzed by LCMS with an Aqueous Normal Phase ANP method - Tips & Suggestions
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Poor peak shape for ethylenediamine in LCMS analyses - Tech Information
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Purge metals from HPLC system using EDTA or medronic acid - How To
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Random shifting of retention times in HPLC chromatograms - Tips & Suggestions
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Restoring the initial results with a method for analyzing AMP ADP ATP UDP and GPT in biological extracts - Tips & Suggestions
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Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run - Tips & Suggestions
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Sample concentration changes can effect retention time in HPLC - Primer
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Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP - FAQ
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ
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Solving baseline problems in Aqueous Normal Phase ANP methods - Tips & Suggestions
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Store and condition Cogent TYPE-C HPLC columns - How To
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Store and maintain Cogent Diamond Hydride HPLC columns - How To
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Suggestions on how to separate capsaicinoids in hot sauce by HPLC - Tips & Suggestions
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The common names of HPLC column diameters - Primer
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The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase - Tips & Suggestions
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Tizanidine HCl HPLC Method transferred to near UHPLC - AppNote
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Use Teflon bottles for A and B solvents in negative ion mode LCMS - Primer
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Using Aqueous Normal Phase ANP to your advantage - White Paper
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies - FAQ
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What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
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What is a master batch for Cogent HPLC columns - FAQ
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What is the compatibility of Cogent TYPE-C Silica HPLC columns with UHPLC instruments - FAQ
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What is the maximum pressure Cogent HPLC columns can tolerate - FAQ
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What is the recommended pH range for Cogent Diamond Hydride HPLC columns - FAQ
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What pressure should I expect with a Diamond Hydride 2.o HPLC column at flow rates from 0.2 to 2.0mL / min - FAQ
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What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column - FAQ
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Which column do you suggest for Cocaine & metabolite Ecognin separation - Tips & Suggestions
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Which size guard column should I use with my 1mm Cogent 2.o column - FAQ
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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Why am I seeing two peaks for quaternium-15 or Dowicil-75 in my LCMS data - FAQ
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Why are the Cogent Silica-C columns more stable than other silica columns - FAQ
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Why do I have lower than normal back pressure with Cogent Diamond Hydride HPLC columns - FAQ
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Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column - FAQ
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