How do retention and efficiency of asymmetric dimethylarginine ADMA compare using 4um vs 2.o Diamond Hydride columns - AppNote
ADMA can be retained using an ANP gradient method with the Cogent Diamond Hydride™ HPLC column.
Retention times for ADMA differed only slightly between the 4um and 2.o™ stationary phases. Efficiency however was notably higher when using the 2.o™ phase. This can be readily observed from the greater peak height using the 2.o™ column in the chromatogram overlay comparison shown below:
Method Conditions:
Column dimensions: 2.1 x 50mm (both)
Flow rate: 0.3mL/min
A: DI H2O + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid
time (min) %B
0 90
5 30
8 30
10 90
post time 4 min
For more information: Cogent Diamond Hydride Product Page
Retention times for ADMA differed only slightly between the 4um and 2.o™ stationary phases. Efficiency however was notably higher when using the 2.o™ phase. This can be readily observed from the greater peak height using the 2.o™ column in the chromatogram overlay comparison shown below:
Method Conditions:
Column dimensions: 2.1 x 50mm (both)
Flow rate: 0.3mL/min
A: DI H2O + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid
time (min) %B
0 90
5 30
8 30
10 90
post time 4 min
For more information: Cogent Diamond Hydride Product Page