Indole-3-Butyric Acid Analyzed with HPLC - AppNote
October 28, 2021
/
/
/
/
/
/
A Reproducible Method for Detection of a Plant Hormone
Click HERE for Column Ordering Information.

A rapid, sensitive, and Reproducible Method has been developed for Analysis of Indole-3-Butyric Acid. The data below, (overlay of 10 chromatograms ) illustrates how the compound can be adequately Retained and detected using a simple Gradient in Reversed Phase HPLC.  The Method demonstrates good Peak Shape and run-to-run Precision with RSD values less than 0.3%.

A Phenyl ring in the Column Stationary Phase provides beneficial π-π Interaction with the Analyte making possible the use of a very simple, Mass Spec friendly Mobile Phase with Formic Acid as an additive.

10 Injections of Indole-3-Butyric Acid Overlay



Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Dimensions: 4.6mm x 75mm
Mobile Phase:
--A: DI Water with 0.1% Formic Acid (v/v)
--B: Acetonitrile with 0.1% Formic Acid (v/v)
Gradient:
Time (minutes) %B
0 25
1 25
5 85
6 85
7 25
10 25
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 280nm
Sample Preparation: Indole-3-Butyric Acid prepared as 1.0mg / mL standard solution in (50:50) Acetonitrile / DI Water

Notes: Indole-3-Butyric Acid a substance that is closely related in structure and function to a natural growth regulator found in plants. Indole-3-butyric acid is used on many crops and ornamentals to promote growth and development of roots, flowers and fruits, and to increase crop yields.

Related Articles

  1. 10 Phenolic Acids Analyzed with LCMS - AppNote
  2. Chlormequat & Mepiquat Analyzed with LCMS - AppNote
  3. Polyphenols in Grape Seed Extract Analyzed with LCMS - AppNote

© Copyright 2025. MICROSOLV. All Rights Reserved. Website & Hosting by BlueTone Media