What are your definitions of normal phase reversed phase and ANP in HPLC - FAQ
April 14, 2020
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We use the short, quick and easy definitions shown below when describing our columns and when offering tips and suggestions.
Normal Phase Chromatography
Using Normal Phase Solvents:
• Most retained compounds are most polar.
• Least retained compounds are less polar.
• More Non Polar Solvents make compounds retain longer.
Reversed Phase Chromatography
Using Reverse Phase Solvents:
• Most retained compounds are least polar.
• Least retained compounds are most polar.
• More Polar Solvents (water) Make compounds retain longer.
Aqueous Normal Phase Chromatography ANP
Using Reversed Phase Solvents:
• Most retained compounds are most polar.
• Least retained compounds are less polar.
• More Non Polar Solvents make compounds retain longer.
Normal Phase Chromatography
Using Normal Phase Solvents:
• Most retained compounds are most polar.
• Least retained compounds are less polar.
• More Non Polar Solvents make compounds retain longer.
Reversed Phase Chromatography
Using Reverse Phase Solvents:
• Most retained compounds are least polar.
• Least retained compounds are most polar.
• More Polar Solvents (water) Make compounds retain longer.
Aqueous Normal Phase Chromatography ANP
Using Reversed Phase Solvents:
• Most retained compounds are most polar.
• Least retained compounds are less polar.
• More Non Polar Solvents make compounds retain longer.