Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP - FAQ
April 14, 2020
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Methanol is generally not recommended for most ANP applications however a few scientists have seen success with it.
Instead, it is best to use polar aprotic solvents such as acetonitrile. If using ELSD or LCMS, acetone can also be a good alternative to acetonitrile as well. However, because of its low UV cutoff, it is generally not appropriate for analyses performed with UV detection.
