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Commercial Rice Extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride Column was an excellent choice to use for analysis of Phenolic Compounds with Reversed Phase HPLC. The Retention and Separation of all nine available standards was possible using the Method below.


Peaks:
1. Gallic Acid
169 m/z [M-H]–
2. 3,4-Hydroxybenzoic Acid
153 m/z
[M-H]–
3. 4-Hydroxybenzoic Acid
137 m/z
[M-H]–
4. Chlorogenic Acid
353 m/z [M-H]–
5. Caffeic Acid
179 m/z [M-H]–
6. Vanillic Acid
167 m/z [M-H]–
7. Syringic Acid
197 m/z [M-H]–
8. p-Coumaric Acid
163 m/z [M-H]–
9. Ferulic Acid
193 m/z [M-H]–
10. 3,5-Dimethoxy-4-Hydroxycinnamic A
cid
223 m/z [M-H]–
Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
-- A: DI Water / 0.1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
Time (minutes) | %B |
0 | 10 |
5 | 20 |
6 | 20 |
7 | 10 |
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – Neg - Perkin Elmer, Flexar SQ 300 Mass Spectrometer
Sample Preparation: Commercial Rice Extract 3 was spiked with standards @ 12.5ppm.
t 0 : 0.5 minutes
Notes: After the Separation Method was developed, several rice extracts were analyzed and compounds from the group of available standards were detected based on m/z values and Retention Time. Peaks for the two compounds Caffeic and Vanillic Acids were not Separated but since these compounds have very different m/z values, it was possible to determine their presence in a rice extract.
Attachment
No 294 10 Phenolic Acids Analyzed with LCMS pdf 0.3 Mb Download File