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Adjusted retention time hold up time - Primer
May 15, 2013
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In the chart below, tR is the retention time and tM is the hold-up time.
Definition: the hold-up time is the time of an analyte (small molecule) which completely penetrates the pores and which is not retained at all by the stationary phase.
Definition: the hold-up time is the time of an analyte (small molecule) which completely penetrates the pores and which is not retained at all by the stationary phase.