4-(Methylamino)butyric Acid Analyzed by HPLC- AppNote
April 23, 2021
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A gamma-Aminobutyric Acid (GABA) Derivative
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 4.6 x 150mm
Mobile Phase:
--A: DI Water with 0.1% Formic Acid
--B: Acetonitrile with 0.1% Formic Acid
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: ELSD (Evaporative Light Scattering Detector)
Sample Preparation: 4-(Methylamino) Butyric Acid 0.1mg / mL in 50:50 Acetonitrile / DI Water
Note: 4-(Methyamino)butyric acid is a GABA (gamma Aminobutyric Acid) derivative and product of N-methyl-2-pyrrolidione. It inhibits L-Carnitine from undergoing beta-oxidation in mammals. In bacteria it is a product of nicotine catabolism. It is found in skin products to prevent wrinkles.
This challenging polar compound is easily retained in this simple Method where repeatability of the data is excellent with %RSD<0.1 for the Retention Times.
Peak:
4-(Methylamino)butyric Acid
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 4.6 x 150mm
Mobile Phase:
--A: DI Water with 0.1% Formic Acid
--B: Acetonitrile with 0.1% Formic Acid
| Time (minutes) | %B |
| 0 | 80 |
| 5 | 30 |
| 6 | 30 |
| 7 | 80 |
Flow rate: 1.0mL / minute
Detection: ELSD (Evaporative Light Scattering Detector)
Sample Preparation: 4-(Methylamino) Butyric Acid 0.1mg / mL in 50:50 Acetonitrile / DI Water
Note: 4-(Methyamino)butyric acid is a GABA (gamma Aminobutyric Acid) derivative and product of N-methyl-2-pyrrolidione. It inhibits L-Carnitine from undergoing beta-oxidation in mammals. In bacteria it is a product of nicotine catabolism. It is found in skin products to prevent wrinkles.