Desloratadine Assay with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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June 1, 2021
Tanya

AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / AppNotes RP C18 / ARTICLES, APPNOTES, FAQ

Alternate L1 Column used for HPLC Method of Desloratadine

This Desloratadine Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values below 0.3%. This demonstrates a good alternate Column for your Desloratadine Method.

Peak:

Desloratadine

Method Conditions:
Column: Cogent Hyper Similars C18™, 5um, 150Å
Catalog No.: 62518-25P
Dimensions: 4.6mm x 250mm
Mobile Phase: 25:75 Acetonitrile / DI Water
Injection vol.: 2μL
Flow rate: 1.0mL / minute
Detection: UV @ 280nm
Sample Preparation: Desloratadine 1.0mg / mL in (50:50) Acetonitrile / DI Water

Note 1: Desloratadine is an antihistamine used to relieve allergy symptoms such as hives, watery eyes, runny nose, itching eyes/nose, sneezing, and itching.

Note 2: The Cogent Hyper Similars C18™ performs very similarly to the ThermoFisher® Hypersil BDS C18®.

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