Amoxicillin USP Assay with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 23, 2021
Tanya

AppNotes Alternate Generic Methods / AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / AppNotes RP C18 / ARTICLES, APPNOTES, FAQ

Alternate L1 Column used for the USP Assay Method for Amoxicillin

This USP Amoxicillin Assay is performed and a five injection overlay shows Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.3%. This demonstrates a good alternate Column for your Amoxicillin USP Method.

Peak:
Amoxicillin

Method Conditions:
Column: Cogent Hyper Similars C18™, 5µm, 150Å
Catalog No.: 62518-25P
Dimensions: 4.6 x 250mm
Mobile Phase: 96:4 Buffer / Acetonitrile
—Buffer: 6.8g / L Monobasic Potassium Phosphate (adjusted with 45% Potassium Hydroxide to a pH of 5.0 +/- 0.1)
Injection vol.: 10μL
Flow rate: 1.0mL / minute
Detection: UV @ 230nm
Sample Preparation: Amoxicillin 1.2mg / mL in Buffer

Note 1: Amoxicillin is a semisynthetic antibiotic and analog of ampicillin, with a broad spectrum of bactericidal activity against many Gram-positive and Gram-negative microorganisms.

Note 2: The Cogent Hyper Similars C18™ performs very similarly to the Thermo Fisher Hypersil® C18.

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