Excellent Peak Shape For a Very Difficult Compound
This Method is easy to prepare, use, reproduce and a good Separation of Aspirin from its major hydrolysis product, Salicylic Acid is achieved.
Peaks:
1. System Peak
2. Aspirin (Acetylsalicylic Acid)
3. Salicylic Acid
Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase: 52% DI Water / 48% Acetonitrile / 0.1% Phosphoric Acid
Temperature: 25°C
Flow rate: 1.5mL / minute
Detection: UV @ 210 nm
Injection vol.: 10μL
Notes: Aspirin, or acetylsalicylic acid (ASA) is a salicylate drug, often used as an analgesic to relieve minor aches and pains, as an antipyretic to reduce fever, and as an anti-inflammatory medication. Aspirin was the first-discovered member of the class of drugs known as non-steroidal anti-inflammatory drugs (NSAIDs), not all of which are Salicylates, although they all have similar effects and most have some mechanism of action which involves non-selective inhibition of the enzyme cyclooxygenase. Today, aspirin is one of the most widely used medications in the world, with an estimated 40,000 metric tons of it being consumed each year.
Attachment
No 86 Aspirin & Salicylic Acid Analyzed with HPLC pdf 0.1 Mb Download File
