Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
December 12, 2012
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When using serum and other biological samples, to keep the columns clean, the A Solvent with DI water and methanol or IPA should be used continuously and not only for conditioning.
We recommend with this mobile phase:
Related article: General solvent conditioning for TYPE-C Silica columns
We recommend with this mobile phase:
- Solvent A: 50:50 DI water / methanol (or 2-propanol) + 0.1% formic acid (for positive mode MS) or +10 mM ammonium acetate or formate (for negative mode MS).
- Solvent B: The same as you would ordinarily use with Aqueous Normal Phase ANP for example, 98% Acetonitrile and 2% DI water with 1-5mM acid or base.
Related article: General solvent conditioning for TYPE-C Silica columns