What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
April 22, 2012
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Although it will vary from column maker to column manufacturer, and is very dependent on the column length back pressure the following is good rule of thumb for common columns.
| Column Dimensions
|
Recommended Flow Rate
|
| 1.0
|
30-60µL/min
|
| 2.1
|
0.1-0.6mL/min
|
| 3.0
|
0.3-1.5mL/min
|
| 4.6
|
0.8-3.0ml/min
|
| 7.8
|
4.0-10mL/min |
