Adjusted retention time in HPLC - HPLC Primer
April 14, 2020
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Adjusted retention time (tR') is the retention time adjusted for the hold-up time: tR' = tR - tM
where tR is the retention time and tM is the hold-up time.
The hold-up time is the time of an analyte (small molecule) which completely penetrates the pores and which is not retained at all by the stationary phase.
