How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
August 28, 2017
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The concentration can be calculated once you have values for the following two equations:
Response Factor (RF) = Peak Area / Concentration
Relative Response Factor (RRF) = RFimpurity / RFAPI
You can use RRF and RFAPI to solve for RFimpurity. Then you can use the measured peak area of the impurity to solve for its concentration.